Synthetic PapRIV was purchased from GL Biochem (Shanghai, China). The alanine scan, metabolites and scrambled control were synthesized using solid-phase peptide synthesis (Supplementary method S1 ). The quality of all peptides was determined using an in-house developed QC method and a purity of 95% or more was found for each sequence. Calcium dichloride dihydrate, magnesium sulphate, potassium chloride, sodium dihydrogen phosphate hydrate, HEPES, sodium lactate and urethane were purchased from Sigma-Aldrich (Diegem, Belgium), while Bovine Serum Albumin (BSA), sodium iodide, sodium dihydrogen phosphate monohydrate were obtained from Merck KGaA (Darmstadt, Germany). Sodium chloride and disodium hydrogen phosphate dihydrate were obtained from VWR (Leuven, Belgium). Calcium dichloride and d- glucose were purchased from Fluka (Diegem, Belgium) and dextran from AppliChem GmbH (Darmstadt, Germany). Water was purified using an Arium 611 Pro VF purification system (Sartorius, Göttingen, Germany) to laboratory-graded water (18.2 MΩ × cm).
Arium 611 pro vf purification system
Manufactured by Sartorius
Sourced in Germany
The Arium 611 Pro VF is a water purification system that produces high-quality ultrapure water for laboratory applications. It utilizes a combination of reverse osmosis and ultraviolet (UV) technologies to remove impurities and ensure consistent water purity.
Automatically generated - may contain errors
Lab products found in correlation
Magnesium sulphate, by Merck Group (3 mentions)
Sodium dihydrogen phosphate hydrate, by Merck Group (3 mentions)
Dextran, by AppliChem (3 mentions)
Sodium lactate, by Merck Group (3 mentions)
Sodium dihydrogen phosphate monohydrate, by Merck Group (3 mentions)
Calcium dichloride dihydrate, by Merck Group (3 mentions)
Sodium chloride (nacl), by Avantor (2 mentions)
Disodium hydrogen phosphate dihydrate, by Avantor (2 mentions)
Synthetic papriv, by GL Biochem (2 mentions)
Hepes, by Merck Group (2 mentions)
Potassium chloride (kcl), by Merck Group (2 mentions)
Sodium iodide, by Merck Group (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Sodium metabisulphite, by Merck Group (1 mentions)
Urethane, by Merck Group (1 mentions)
Iodo gen coated tubes, by Thermo Fisher Scientific (1 mentions)
Disodium hydrogen phosphate dihydrate, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Chloramine t, by Merck Group (1 mentions)
3 protocols using arium 611 pro vf purification system
1
Synthetic PapRIV Characterization
2
Radiolabeling Peptides for Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Calcium dichloride dihydrate, magnesium sulphate, potassium chloride, sodium chloride, sodium dihydrogen phosphate hydrate, sodium lactate and urethane were purchased from Sigma-Aldrich (Diegem, Belgium), while Bovine Serum Albumin (BSA), disodium hydrogen phosphate dihydrate, sodium iodide, sodium dihydrogen phosphate monohydrate, sodium metabisulphite and Chloramine-T were obtained from Merck KGaA (Darmstadt, Germany). Calcium dichloride, D-glucose, formic acid (FA) and HEPES were purchased from Fluka (Diegem, Belgium) and dextran from AppliChem GmbH (Darmstadt, Germany). For the mobile phases, acetonitrile was obtained from Fisher Scientific (Erembodegem, Belgium) and water was purified using an Arium 611 Pro VF purification system (Sartorius, Göttingen, Germany) to laboratory-graded water (18.2 MΩ × cm). For the radiolabeling of the peptides, Iodo-Gen® coated tubes were purchased from Thermo Scientific (Erembodegem, Belgium) and the radioactive sodium iodide solution (Na125I) from Perkin Elmer (Zaventem, Belgium).
3
Synthetic PapRIV Peptide Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Synthetic PapRIV was purchased from GL Biochem (Shanghai, China). The alanine scan, metabolites and scrambled control were synthesized using solid-phase peptide synthesis (Supplementary method S1). The quality of all peptides was determined using an in-house developed QC method and a purity of 95% or more was found for each sequence. Calcium dichloride dihydrate, magnesium sulphate, potassium chloride, sodium dihydrogen phosphate hydrate, HEPES, sodium lactate and urethane were purchased from Sigma-Aldrich (Diegem, Belgium), while Bovine Serum Albumin (BSA), sodium iodide, sodium dihydrogen phosphate monohydrate were obtained from Merck KGaA (Darmstadt, Germany). Sodium chloride and disodium hydrogen phosphate dihydrate were obtained from VWR (Leuven, Belgium).
Calcium dichloride and D-glucose were purchased from Fluka (Diegem, Belgium) and dextran from AppliChem GmbH (Darmstadt, Germany). Water was purified using an Arium 611 Pro VF purification system (Sartorius, Göttingen, Germany) to laboratory-graded water (18.2 MΩ × cm).
Calcium dichloride and D-glucose were purchased from Fluka (Diegem, Belgium) and dextran from AppliChem GmbH (Darmstadt, Germany). Water was purified using an Arium 611 Pro VF purification system (Sartorius, Göttingen, Germany) to laboratory-graded water (18.2 MΩ × cm).
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