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Ab175670

Manufactured by Abcam
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Sourced in United States

Ab175670 is a lab equipment product. It is a device used for specific laboratory applications. The core function of this product is to perform tasks related to scientific research and analysis.

Automatically generated - may contain errors

Lab products found in correlation

Ab18450, by Abcam (2 mentions) Fluorobrite dmem media, by Thermo Fisher Scientific (2 mentions) Facsdiva v8, by BD (2 mentions) Lsrfortessa, by BD (2 mentions)

2 protocols using ab175670

1

Integrin β1 Activation Measurement

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About 250 000 cells per sample were spun into a 96‐well plate. They were resuspended in 200 µL DMEM Fluorobrite media (A1896701; Thermo Fisher Scientific)—for active integrin β1 1.8 mM of CaCl2 and 1 mM of MgCl2 were added to the media to stabilize the conformation––with the appropriate antibodies (integrin β1 1:50; Active β1 9EG7 1:2000) for 1 hour. They were washed in media then incubated with secondary antibodies conjugated to fluorophores (Anti‐rat Alexa 405; ab175670; 1:100; Abcam, Cambridge, MA, USA) for 30 minutes then washed. Gating was set using unlabeled controls, IgG controls (Rat IgG2, kappa monoclonal; ab18450; Abcam), and secondary only controls. All samples were analyzed using a Becton Dickinson (BD) Special Order Research Product (SORP) LSR Fortessa equipped with lasers at the following wavelengths: 405, 488, 561, and 640 nm. Samples were acquired using BD FACSDiva v 8.1.
Saraswati S., Lietman C.D., Li B., Mathew S., Zent R, & Young P.P. (2020). Small proline‐rich repeat 3 is a novel coordinator of PDGFRβ and integrin β1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts. The FASEB Journal, 34(6), 7885-7904.
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2

Flow Cytometry of Active Integrin β1

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250,000 cells per sample were spun into a 96-well plate. They were resuspended in 200 μl DMEM Fluorobrite media (A1896701; Thermo Fisher Scientific)—for active integrin β1 1.8 mM CaCl2 and 1 mM MgCl2 were added to the media to stabilize the conformation – with the appropriate antibodies (integrin β1 1:50; Active β1 9EG7 1:2000) for 1 hour. They were washed in media then incubated with secondary antibodies conjugated to fluorophores (Anti-rat Alexa 405; ab175670; 1:100; Abcam, Cambridge, MA, USA) for 30 minutes then washed. Gating was set using unlabeled controls, IgG controls (Rat IgG2, kappa monoclonal; ab18450; Abcam), and secondary only controls. All samples were analyzed using a Becton Dickinson (BD) Special Order Research Product (SORP) LSR Fortessa equipped with lasers at the following wavelengths: 405nm, 488nm, 561nm, 640nm. Samples were acquired using BD FACSDiva v 8.1.
Saraswati S., Lietman C.D., Li B., Mathew S., Zent R, & Young P.P. (2020). Small proline‐rich repeat 3 is a novel coordinator of PDGFRβ and integrin β1 crosstalk to augment proliferation and matrix synthesis by cardiac fibroblasts. The FASEB Journal, 34(6), 7885-7904.
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