100 nm polycarbonate membrane filter

Manufactured by Cytiva

Sourced in United Kingdom

The 100 nm polycarbonate membrane filter is a lab equipment product designed for filtration applications. It has a nominal pore size of 100 nanometers, allowing it to effectively filter out particles and microorganisms larger than this size. The filter is made of polycarbonate, a durable and chemically resistant material.

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Lab products found in correlation

Bio beads sm 2, by Bio-Rad (1 mentions) Pd 10 column, by GE Healthcare (1 mentions) Mini extruder set, by Avanti Polar Lipids (1 mentions)

Close spelling variants of this product

100 nm polycarbonate membranes Polycarbonate membrane filters 100 nm membrane 100-nm filters Polycarbonate membranes Polycarbonate filter Membrane filter

3 protocols using 100 nm polycarbonate membrane filter

Preparation and Reconstitution of SUV Proteoliposomes

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Small unilamellar vesicles (SUVs) were prepared from 1-palmitoyl-2-oleoyl-glycero-3-phosphocholine (POPC), 1,2-dioleoyl-sn-glycero-3-phospho-l-serine (DOPS) and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2,3-benzoxadiazol-4-yl) (NBD-PE) at a molar ratio of 84:15:1. Lipids were mixed in chloroform and dried to obtain a thin film, then placed under vacuum for 3 h to remove residual organic solvent. The resulting lipid film was resuspended in Buffer A to a total lipid concentration of 10 mM. The lipid suspension was repeatedly frozen in liquid nitrogen and thawed in warm water for ten cycles. The lipid solution was extruded using a mini-extruder (Avanti) with 100-nm polycarbonate membrane filter (Whatman) by repeatedly passing through the membrane filter for 11 times.
Proteoliposomes were prepared by reconstitution of proteins into SUVs using a detergent-mediated method. SUVs were diluted to a total lipid concentration of 2 mM, and DDM was added to a final concentration of 0.11% according to the detergent-saturated concentration of liposomes. Then proteins were added to the mixture and incubated at 4°C for 1 h. For complete detergent removal, four sequential additions were made every hour at 4°C with a fifth addition for overnight incubation using Bio-Beads SM-2 (Bio-Rad). Bio-Beads SM-2 were gradually added at increasing amounts each time.

Fang J., Zhang Y., Zhu T, & Li Y. (2023). Scramblase activity of proteorhodopsin confers physiological advantages to Escherichia coli in the absence of light. iScience, 26(12), 108551.

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Preparation of Stealth Copper-Loaded Liposomes

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Stealth liposomes were prepared by the hydration-extrusion method by incorporating 5% PEGylated lipids (DPPE-PEG₂₀₀₀) into the total phospholipid concentration (17 (link)). Briefly, lipid mixtures of DPPC, cholesterol and DPPE-PEG₂₀₀₀ at a molar ratio of 80:50:4 (µmol) were dissolved in chloroform and the solvent was evaporated on a rotary evaporator under vacuum. Non-PEGylated liposomes were prepared without the addition of DPPE-PEG₂₀₀₀. Next, 100 mM (or other concentrations) of CuCl₂ (or CuSO₄) solution was used to hydrate the thin film of lipids that formed on the wall of the round bottom flask, while rotating in a 55 °C water bath for 30 minutes. The resulting multilamellar vesicle (MLV) suspension was subjected to three freeze-thaw cycles before being extruded 11 times through a 100 nm polycarbonate membrane filter (Whatman, Springfield Mill, UK) via a Mini-Extruder Set (Avanti Polar Lipids) to generate large unilamellar vesicles (LUVs) encapsulating the Cu salt. Residual un-encapsulated Cu was removed by size exclusion chromatography by passing the suspension through a desalting PD-10 column (GE Healthcare, Waukesha, WI) and eluting with either ddH₂O (for short-term storage and in vitro experiments) or 0.9% sodium chloride (for in vivo administrations into mice). Saline liposomes were prepared by encapsulating normal saline solution instead of CuCl₂ solution.

Wang Y., Zeng S., Lin T.M., Krugner-Higby L., Lyman D., Steffen D, & Xiong M.P. (2014). Evaluating the Anticancer Properties of Liposomal Copper in a Nude Mouse Xenograft Model of Human Prostate Cancer: Formulation, In Vitro, In Vivo, Histology and Tissue Distribution Studies. Pharmaceutical research, 31(11), 3106-3119.

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Preparation and Characterization of SECosomes

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SECosomes and derivatives (Table 1) were prepared using the solvent evaporation method (Geusens et al., 2010) . Lipids were dissolved in chloroform, TW20/80 in sterile distilled water, and SC in >99% EtOH by means of sonication. After mixing the components, solvent was removed by rotary vacuum evaporation above lipid transition temperature. The resulting film was hydrated in HB with 30% EtOH. After overnight incubation, vesicles were extruded 31Â through a 100 nm polycarbonate membrane filter (Whatman, Brentfort, UK). Corresponding lipoplexes (LPX) were prepared by diluting the RNAi molecules with HB to a volume of 100 mL. Liposomes were added under vortex mixing to a liposome/ siRNA or antagomiR ratio of 16/1 (w/w), and liposome/pre-miR of 8/1. Average particle size and zeta potential were determined using Zetasizer Nano series (Malvern, Worcestershire, UK).

Desmet E., Bracke S., Forier K., Taevernier L., Stuart M.C., De Spiegeleer B., Raemdonck K., Van Gele M, & Lambert J. (2016). An elastic liposomal formulation for RNAi-based topical treatment of skin disorders: Proof-of-concept in the treatment of psoriasis. International journal of pharmaceutics, 500(1-2).

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