Pe conjugated rat anti cd49f integrin α6

The following primary antibodies were used and combined according to species compatibility: rabbit anti-GluA4C, targeting the C-terminus of GluA4 (1:250, #AB1508, EMD Millipore, Billerica, MA, USA), guinea pig anti-GluA4N, targeting the N-terminus of GluA4 (1:50 for immunohistochemistry #GluA4N-GP-Af640, Frontier Institute Co., Ltd, Hokkaido, Japan), Alexa647-conjugated rat anti-CD34 (1:50 for immunohistochemistry; 1:100 for FACS, #560233, BD Biosciences, San Diego, CA, USA), PE-conjugated rat anti-CD49f-integrin α6 (1:100, #555736, BD Biosciences, San Jose, CA, USA), FITC-conjugated rat anti-Ly-6A/E (Sca-1) (1:100, #11-5981-81, E Bioscience, San Diego, CA, USA), rabbit anti-Loricrin (1:1000, #PRB-145P, BioLegend, Dedham, MA, USA), rabbit anti-TSLP (1:250, #ab47943, Abcam Inc, Cambridge, MA, USA). Alexa Fluor-conjugated secondary antibodies were all obtained from Invitrogen-Molecular Probes, and used at 1:1000 (#A11008 Alexa Fluor 488 Goat Anti-Rabbit (H+L), #A21450 Alexa Fluor 647 goat anti-guinea pig, and #A11005Alexa Fluor 594 Goat anti-mouse (H+L)). In addition to validation by mRNA expression, immunolabeling experimental controls included omission of primary antibodies.