Milliplex analyst software version 4

Manufactured by Merck Group

Milliplex Analyst software version 4.2 is a data analysis software designed for use with Millipore's Milliplex assays. The software provides tools for data acquisition, visualization, and analysis of multiplex assay results. It is compatible with various Milliplex instrument platforms.

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Lab products found in correlation

Hcytmag 60k px38, by Merck Group (2 mentions) Luminex 200, by Merck Group (1 mentions) Human 38 plex magnetic cytokine chemokine kits, by Merck Group (1 mentions)

Spelling variants of this product

Milliplex Analyst software (80 citations) Milliplex Analyst (30 citations) Analyst software (11 citations) Milliplex Software (5 citations)

3 protocols using milliplex analyst software version 4

Multiplex Cytokine/Chemokine Profiling

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Human 38-plex magnetic cytokine/chemokine kits (EMD Millipore, HCYTMAG-60K-PX38) were used per manufacturer's instructions. The panel includes IL-1RA, IL-10, IL-1ɑ, IL- 1β, IL-6, IFN-ɑ2, TNF-β, TNF-ɑ, sCD40L, IL-12p40, IFN-γ, IL-12/IL-12p70, IL-4, IL-5, IL-13, IL-9, IL-17A, GRO/CXCL1, IL-8/CXCL8, eotaxin/CCL11, MDC/CCL22, fractalkine/CX3CL1, IP-10/CXCL10, MCP-1/CCL2, MCP-3/CCL7, MIP-1ɑ/CCL3, MIP-1β/CCL4, IL-2, IL-7, IL-15, GM-CSF, Flt-3L/CD135, G-CSF, IL-3, EGF, FGF-2, TGF-β, and VEGF. Fluorescence was quantified using a Luminex 200TM instrument. Cytokine/chemokine concentrations were calculated using Milliplex Analyst software version 4.2 (EMD Millipore). Luminex assay and analysis were performed by the UCLA Immune Assessment Core.

Chin J.L., Tan Z.C., Chan L.C., Ruffin F., Parmar R., Ahn R., Taylor S.D., Bayer A.S., Hoffmann A., Fowler VG J.r., Reed E.F., Yeaman M.R, & Meyer A.S. (2024). Tensor modeling of MRSA bacteremia cytokine and transcriptional patterns reveals coordinated, outcome-associated immunological programs. PNAS Nexus, 3(5), pgae185.

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Multiplex Cytokine/Chemokine Profiling

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Luminex assay and analysis were performed by the University of California, Los Angeles Immune Assessment Core. Human 38-plex magnetic cytokine/chemokine kits (EMD Millipore; HCYTMAG-60K-PX38) were used per the manufacturer’s instructions. Fluorescence was quantified using a Luminex 200 instrument. Cytokine/chemokine concentrations were calculated using Milliplex Analyst software version 4.2 (EMD Millipore).

Mba Medie F., Sharma-Kuinkel B.K., Ruffin F., Chan L.C., Rossetti M., Chang Y.L., Park L.P., Bayer A.S., Filler S.G., Ahn R., Reed E.F., Gjertson D., Yeaman M.R, & Fowler VG J.r. (2019). Genetic variation of DNA methyltransferase-3A contributes to protection against persistent MRSA bacteremia in patients. Proceedings of the National Academy of Sciences of the United States of America, 116(40), 20087-20096.

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Cytokine/Chemokine Profiling in Transplant Recipients

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Peripheral venous blood samples from 11 IFLT recipients and 14 CLT recipients were collected using EDTA as an anticoagulant at 12 h after graft revascularization. The plasma samples obtained by centrifugation for 10 min at 1000×g were stored at ‐20°C and completely dissolved before use. The assay of cytokine/chemokine measurement was directed by the manufacturer's instructions of Human 38‐plex magnetic cytokine/chemokine kits (EMD Millipore, HCYTMAG‐60K‐PX38). Fluorescence was quantified using a Luminex 200 instrument, and the concentrations were calculated by Milliplex Analyst software version 4.2 (EMD Millipore). All samples were examined in triplicate.

Guo Z., Xu J., Huang S., Yin M., Zhao Q., Ju W., Wang D., Gao N., Huang C., Yang L., Chen M., Zhang Z., Zhu Z., Wang L., Zhu C., Zhang Y., Tang Y., Chen H., Liu K., Lu Y., Ma Y., Hu A., Chen Y., Zhu X, & He X. (2022). Abrogation of graft ischemia‐reperfusion injury in ischemia‐free liver transplantation. Clinical and Translational Medicine, 12(4), e546.

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