Rac1 activity assay kit

Manufactured by Cell Biolabs

The Rac1 Activity Assay Kit is a laboratory equipment product that measures the activation state of the Rac1 protein. The kit provides a simple and reliable method to detect and quantify the level of active, GTP-bound Rac1 in cell and tissue samples.

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Lab products found in correlation

Ab109381, by Abcam (2 mentions) Prohibitin, by Santa Cruz Biotechnology (2 mentions) Ab40660, by Abcam (2 mentions) Ab33770, by Abcam (2 mentions) Ab86695, by Abcam (2 mentions) Ab52640, by Abcam (2 mentions) Ab68166, by Abcam (2 mentions) Ab133504, by Abcam (1 mentions) Ab205719, by Abcam (1 mentions) L glutamate, by Merck Group (1 mentions) Ab7090, by Abcam (1 mentions) Caspase 3 activity assay kit, by Abcam (1 mentions) Goat anti mouse igg h l, by Abcam (1 mentions) Amplex red hydrogen peroxide peroxidase assay kit, by Thermo Fisher Scientific (1 mentions) Dcfh da, by Merck Group (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) β actin, by Santa Cruz Biotechnology (1 mentions) Ab7260, by Abcam (1 mentions) Sc 377037, by Santa Cruz Biotechnology (1 mentions)

3 protocols using rac1 activity assay kit

Rac1 Activation Assay Protocol

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The Rac1 activation assay was performed using Cell Biolabs Rac1 Activity Assay Kit (#STA-401–1). Briefly, cells or tissue were lysed as described above and the active form of Rac1 (GTP-Rac1) was selectively pulled down from the lysate with p21-binding domain (PBD) of PAK agarose beads. Subsequently, the precipitated GTP-Rac1 was detected by Western blot analysis as described above. Each assay also consisted of lysates that were loaded with GTPγS or GDP as positive and negative controls respectively. A separate Western blot was run to evaluate total levels of Rac1 in each lysate.

Festa L.K., Irollo E., Platt B.J., Tian Y., Floresco S, & Meucci O. (2020). CXCL12-induced rescue of cortical dendritic spines and cognitive flexibility. eLife, 9, e49717.

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Molecular Mechanisms of GBE-Mediated Cytoprotection

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GBE was provided by Wanbangde Pharmaceutical Group Co., Ltd. Chemicals such as L-glutamate, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide), DCFH-DA and DMSO were obtained from Sigma-Aldrich; Merck KGaA. Antibody were obtained from Santa Cruz Biotechnology, Inc. or Abcam: p-SRC (ab40660, Abcam), SRC (ab109381, Abcam), p-VAV2 (ab86695, Abcam), Vav2 (ab52640, Abcam), p-p66Shc (ab68166, Abcam), p66Shc (ab33770, Abcam), cytochrome c (ab133504, Abcam), β-actin (sc-58673, Santa Cruz Biotechnology, Inc.), prohibitin (sc-377037, Santa Cruz Biotechnology, Inc.), Goat Anti-Rabbit IgG H&L (HRP) (ab7090, Abcam), Goat Anti-Mouse IgG H&L (HRP) (ab205719, Abcam). Rac1 activity assay kit was obtained from Cell Biolabs. Amplex Red hydrogen peroxide/peroxidase assay kit was obtained from Thermo Fisher Scientific, Inc. Caspase-3 Activity assay kit was obtained from Abcam. Other chemicals and reagents used in this study were obtained from Beyotime.

Wang K., Ni J., Zhu X., Zhu L., Li Y, & Zhou F. (2021). Ginkgo biloba extract protects human neuroblastoma SH-SY5Y cells against oxidative glutamate toxicity by activating redoxosome-p66Shc. Experimental and Therapeutic Medicine, 22(3), 951.

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Investigating Oxidative Stress Pathways

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Chemicals such as RAC1 inhibitor NSC23766 and NOX (NADPH oxidase) inhibitor VAS2870 were purchased from MCE Biotechnology. MTT (3-(4,5-Dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide) cell proliferation assay kit was purchased from Solarbio. Reagents such as antibodies were purchased from Abcam, Santa Cruz Biotechnology and NOVUS: p-SRC (ab40660, Abcam), SRC (ab109381, Abcam), p-Vav2 (ab86695, Abcam), Vav2 (ab52640, Abcam), p-p66SHC (ab68166, Abcam), p66SHC (ab33770, Abcam), GAPDH (ab8245, Abcam), Prohibitin (sc-377037, Santa Cruz Biotechnology), NOX4 (NB110-58849, NOVUS), GFAP (ab7260, Abcam). RAC1 activity assay kit was purchased from Cell Biolabs. NOX activity assay kit, MDA (malondialdehyde) assay kit and SOD (super oxide dismutase) activity assay kit were purchased from Solarbio and Abcam. Other chemicals and reagents used in this study were obtained from Beyotime and Sangon.

Jiang Y., Xu L., Zhu X., Zhu X., Xu X, & Li J. (2024). Hyperglycemic stress induces oxidative damage of enteric glial cells by triggering redoxosomes/p66SHC activation. Redox Report : Communications in Free Radical Research, 29(1), 2324234.

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