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Vydac 201 tp c18 5

Manufactured by Grace Bio-Labs
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The Vydac 201 TP C18 5µ is a reversed-phase high-performance liquid chromatography (HPLC) column. It has a stationary phase composed of octadecylsilane (C18) bonded to porous silica particles with a diameter of 5 micrometers. This column is designed for the separation and analysis of a wide range of organic compounds.

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Lab products found in correlation

Hplc system, by Grace Bio-Labs (2 mentions) Vydac 201 tp, by Grace Bio-Labs (2 mentions)

Spelling variants of this product

C18 Vydac (4 citations) Vydac 201 TP (3 citations)

2 protocols using vydac 201 tp c18 5

1

HPLC Analysis of Organic Compounds

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HPLC analysis was performed using a Waters Alliance HPLC System with a Vydac 201 TP C18 5µ (250 mm × 2.1 mm) column equipped with a guard column (Vydac 201 TP, Grace), and a UV detector set at 341 nm. The mobile phase consisted of acetonitrile:water (80:20, v/v).The flow rate was 0.3 mL/min with a retention time 3.65 minutes and the total run time of each analysis was 7 minutes at ambient temperature. The injection volume was 10 µL. Empower software was used for the integration of the peak areas.
Benbrook D.M., Janakiram N.B., Chandra V., Pathuri G., Madka V., Stratton N.C., Masamha C.P., Farnsworth C.N., Garcia-Contreras L., Hatipoglu M.K., Lighfoot S, & Rao C.V. (2017). Development of a dietary formulation of the SHetA2 chemoprevention drug for mice. Investigational New Drugs, 36(4), 561-570.
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2

HPLC Analysis of SHetA2 in Solutions

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Determination of SHetA2 in solutions was performed using a Waters Alliance HPLC
System with a Vydac 201 TP C18 5µ (250 mm×2.1 mm)
column equipped with a guard column (Vydac 201 TP, Grace), and a UV detector
set at 341 nm. The mobile phase consisted of acetonitrile:water (80:20, v/v).The flow rate
was 0.3 mL/min with a retention time of 3.65 minutes.
Mahjabeen S., Hatipoglu M.K., Chandra V., Benbrook D.M, & Garcia-Contreras L. (2017). Optimization of a Vaginal Suppository Formulation to Deliver SHetA2 as a Novel Treatment for Cervical Dysplasia. Journal of pharmaceutical sciences, 107(2), 638-646.
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