An Annexin V-FITC/propidium iodide (PI) double staining kit (Yeasen Biotech Co., Ltd, Shanghai, China) was used to detect ESCC cell apoptosis. Briefly, after the medium was discarded, the cells were rinsed twice with pre-chilled PBS and resuspended in 1× binding buffer. Following the addition of 5 μL of Annexin V-FITC staining solution and 5 μL of PI solution, the cell suspension was mixed thoroughly and incubated for 15 min in the dark; the apoptotic cells were determined using a flow cytometer (BD Biosciences, San Jose, CA, USA) within 1h.
Annexin 5 fitc propidium iodide double staining kit
Manufactured by Yeasen
Sourced in China
The Annexin V-FITC/Propidium iodide (PI) double staining kit is a laboratory reagent used for the detection and quantification of apoptotic and necrotic cells. It contains Annexin V-FITC and Propidium Iodide (PI) as the main components. Annexin V-FITC binds to phosphatidylserine, which is exposed on the cell surface during early apoptosis, while PI stains the DNA of cells with compromised cell membranes, indicating late apoptosis or necrosis.
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3 protocols using annexin 5 fitc propidium iodide double staining kit
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Annexin V-FITC/PI Apoptosis Assay
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Annexin V-FITC/PI Apoptosis Assay
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The Annexin V-FITC/Propidium iodide (PI) double staining kit (YEASEN, Shanghai, China) was employed to examine cell apoptosis in keeping with the protocol provided by the manufacturer. Briefly, the cells were harvested and stained with Annexin V-FITC and PI respectively, the FCM (Partec, Germany) was employed to measure cell apoptosis ratio. The FITC was detected at 530 nm, and PI was detected at 575 nm, respectively.
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Apoptosis Evaluation by Annexin V-FITC/PI
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The Annexin V-FITC/Propidium iodide (PI) double staining kit (YEASEN, China) was employed to examine cell death in keeping with the protocol provided by the manufacturer. Briefly, the cells of different treated groups were harvested and resuspended into 500 μL binding buffer. Then, cells were incubated with Annexin V at 4 °C for 30 min in the dark and further stained with PI at room temperature for 5 min. Cells were finally determined via flow cytometer (Agilent, China).
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