The alpha myosin heavy chain (αMHC) promoter-driven MerCreMer (MCM), purchased from the Jackson Laboratory, were crossed with floxed SVV transgenic mice 24 (link). Wild-type C57BL/6 mice (WT) were purchased from National Applied Research Laboratories, National Laboratory Animal Center, Taiwan. Male animals aged 8-10 weeks were regarded as adult mice in all the experiments.
Mercremer
Manufactured by Jackson ImmunoResearch
MerCreMer is a protein that facilitates the recombination and excision of DNA sequences. It functions as a site-specific recombinase, enabling the targeted manipulation of genetic material.
Automatically generated - may contain errors
Lab products found in correlation
Myh6 cre, by Jackson ImmunoResearch (1 mentions)
Smad4fl fl mice, by Jackson ImmunoResearch (1 mentions)
C57bl 6 background, by Jackson ImmunoResearch (1 mentions)
Fvb tg csf1r cre esr1 1jwp j, by Jackson ImmunoResearch (1 mentions)
B6 cg speer6 ps1tg alb cre 21mgn j, by Jackson ImmunoResearch (1 mentions)
4 protocols using mercremer
1
Genetic Manipulation of Mice
2
Generation of Cardiomyocyte-Specific SMAD4 Knockout Mice
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To achieve conditional deletion of SMAD4 specifically in CM, SMAD4fl/fl mice (Stock No. 017462, The Jackson Laboratory, Bar Harbor, Maine) were crossed with mice carrying the MerCreMer (MCM) transgene driven by the alpha-myosin heavy chain promoter (Myh6-Cre+/+) (Stock No. 005657, The Jackson Laboratory). Further details of generation and characterization of the CM-specific SMAD4 knockout (KO) mice are described in the Results section. The Institutional Animal Care and Use Committee of Vanderbilt University Medical Center approved all animal procedures and treatments used in this study (protocol #M1700133-00).
3
Transgenic Mice for Cardiac Research
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The investigation conforms to the Guide for the Use and Care of Laboratory Animals, and all of the animal protocols were approved by the Institutional Animal Care and Use Committees of National Cheng Kung University and Academia Sinica.
The α‐MHC promoter‐driven MerCreMer, Z/EG transgenic mice, and the EP2 heterozygous (EP2+/−) mice in the C57BL/6 background were purchased from the Jackson Laboratory. The EP2+/− mice were mated to generate EP2‐null (EP2−/−) mice. The double‐transgenic MCM/ZEG (MZ) mice were generated by mating the MCM to Z/EG. The MZ females were mated with EP2−/− males to generate MCM and Z/EG heterozygous for EP2. The triple transgenic EP2−/−/MZ mice were generated by mating the EP2+/− MCM and EP2+/− Z/EG mice. The wild‐type (WT) mice in the C57BL/6J background were purchased from the National Laboratory Animal Center (Taiwan). Adult male and female animals of 12 to 18 weeks of age were used for the experiments conducted in this study.
The α‐MHC promoter‐driven MerCreMer, Z/EG transgenic mice, and the EP2 heterozygous (EP2+/−) mice in the C57BL/6 background were purchased from the Jackson Laboratory. The EP2+/− mice were mated to generate EP2‐null (EP2−/−) mice. The double‐transgenic MCM/ZEG (MZ) mice were generated by mating the MCM to Z/EG. The MZ females were mated with EP2−/− males to generate MCM and Z/EG heterozygous for EP2. The triple transgenic EP2−/−/MZ mice were generated by mating the EP2+/− MCM and EP2+/− Z/EG mice. The wild‐type (WT) mice in the C57BL/6J background were purchased from the National Laboratory Animal Center (Taiwan). Adult male and female animals of 12 to 18 weeks of age were used for the experiments conducted in this study.
4
Conditional Genetic Manipulation of Mice
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All animals were maintained in the specific pathogen-free Laboratory Animal Center of Nanjing Medical University according to the guidelines of the Institutional Animal Care and Use Committee at Nanjing Medical University, Nanjing, China.
Male C57BL/6 mice weighing 18–22 g were acquired from the specific pathogen-free Laboratory Animal Center of Nanjing Medical University.
Homozygous Tsc1 floxed mice (005680, The Jackson Laboratory, Bar Harbor, ME) were ordered from Jackson Laboratory. Rheb floxed mice were kindly provided by Dr. Bo Xiao (Southern University of Science and Technology, China) [18 (link), 19 (link)]. Mice expressing the tamoxifen-inducible MerCreMer fusion protein under the control of macrophage specific mouse Csf1r promoter were ordered from Jackson Laboratory (019098, FVB-Tg(Csf1r-cre/Esr1*)1Jwp/J, donated from Jeffrey Pollard, The Jackson Laboratory, Bar Harbor, ME) [50 (link)]. Alb-Cre mice (003574, B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J, The Jackson Laboratory, Bar Harbor, ME) were ordered from Jackson Laboratory. Male and female mice between 6 and 8 weeks of age were used in this study.
Male C57BL/6 mice weighing 18–22 g were acquired from the specific pathogen-free Laboratory Animal Center of Nanjing Medical University.
Homozygous Tsc1 floxed mice (005680, The Jackson Laboratory, Bar Harbor, ME) were ordered from Jackson Laboratory. Rheb floxed mice were kindly provided by Dr. Bo Xiao (Southern University of Science and Technology, China) [18 (link), 19 (link)]. Mice expressing the tamoxifen-inducible MerCreMer fusion protein under the control of macrophage specific mouse Csf1r promoter were ordered from Jackson Laboratory (019098, FVB-Tg(Csf1r-cre/Esr1*)1Jwp/J, donated from Jeffrey Pollard, The Jackson Laboratory, Bar Harbor, ME) [50 (link)]. Alb-Cre mice (003574, B6.Cg-Speer6-ps1Tg(Alb-cre)21Mgn/J, The Jackson Laboratory, Bar Harbor, ME) were ordered from Jackson Laboratory. Male and female mice between 6 and 8 weeks of age were used in this study.
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