A total of 91 male BALB/cJ mice were used in this study. Five cohorts of 10 week old mice were obtained from Jackson Laboratories (Bar Harbor, ME). Upon arrival, mice were individually housed, and acclimated to the facility for 1 week before being assigned the experimental diets. During that first week, all mice were fed 8640 Teklad 22/5 rodent diet (Harlan Teklad, Indianapolis, IN). Mice were housed under a reverse 12h light-dark cycle with lights on at 10 PM and off and 10 AM. Autoclaved water was provided ad libitum at all times. All procedures were approved by the University of Illinois Institutional Animal Care and Use Committee and adhered to NIH guidelines. All measures were taken to minimize the number of mice used as well as the pain and suffering of the mice. The University of Illinois at Urbana-Champaign is accredited by the Association for Assessment and Accreditation of Laboratory Animal Care International.
8640 teklad 22 5 rodent diet
Manufactured by Inotiv
Sourced in United States
The 8640 Teklad 22/5 rodent diet is a complete and balanced feed formulation designed for the nutrition of rodents in a laboratory setting. It provides the necessary nutrients, vitamins, and minerals to support the health and well-being of rodents used in research.
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4 protocols using 8640 teklad 22 5 rodent diet
1
BALB/cJ Mice Dietary Acclimation
2
Aged BALB/cByJ Mice Housing and Care
3
Dihydrotestosterone-Induced Metabolic Disorders
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Three-week-old female Sprague Dawley rats were obtained from Envigo (Indianapolis, IN, USA). At 4 weeks of age, rats were randomly assigned to be implanted subcutaneously with continuous-release of dihydrotestosterone (DHT) pellets (7.5 mg/90 days; Innovative Research of America, Sarasota, FL, USA) or sham surgery (Control) as we previously reported [14 (link)]. Rats were maintained on a standard rat chow diet (Teklad 22/5 Rodent Diet #8640; Envigo, Indianapolis, IN, USA), housed in temperature-controlled rooms with ad libitum food and water, and a constant light/dark cycle (12 h/12 h). Animals were followed for 90 days. All experimental protocols were performed following the National Institutes of Health Guide for the Care and Use of Laboratory Animals, 8th Edition, 2011, and approved by the Institutional Animal Care and Use Committee of the University of Mississippi Medical Center.
As we previously reported [14 (link)], the sodium–glucose cotransporter-2 inhibitor, EMPA (10 mg/kg/day, AChemBlock, CA, USA), was administered in drinking water at a dose shown to be effective in lowering BP and hemoglobin A1c in other rodent models [38 (link), 39 (link)]. EMPA treatment was administrated during the last 3 weeks of the experimental protocol. During EMPA treatment, fluid intake was measured daily, and body weight was measured twice a week to dose EMPA appropriately each day.
As we previously reported [14 (link)], the sodium–glucose cotransporter-2 inhibitor, EMPA (10 mg/kg/day, AChemBlock, CA, USA), was administered in drinking water at a dose shown to be effective in lowering BP and hemoglobin A1c in other rodent models [38 (link), 39 (link)]. EMPA treatment was administrated during the last 3 weeks of the experimental protocol. During EMPA treatment, fluid intake was measured daily, and body weight was measured twice a week to dose EMPA appropriately each day.
4
Dihydrotestosterone Exposure in Sprague Dawley Rats
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Female Sprague Dawley rats were obtained from Envigo (Indianapolis, IN) at 3 weeks of age. Rats were maintained throughout the study on standard rat chow diet (Teklad 22/5 Rodent Diet #8640; Envigo), housed in temperature-controlled rooms with a constant light/dark cycle (12 h/12 h) and free access to food and water. At 4 weeks of age, rats were randomly assigned to be implanted subcutaneously on the back of the neck with a continuous-release DHT (7.5 mg/90 days; Innovative Research of America, Sarasota, FL) or placebo pellets (n = 10 per group), as we previously reported [29 (link)]. Pellets were replaced every 90 days. After 6 months of DHT or placebo exposure, pellets were discontinued (ex-DHT), and animals were followed for an additional 6 months and compared with control groups. At the end of the study, blood samples and tissues were collected.
All experimental protocols were performed in accordance with the NIH Guide for the Care and Use of Laboratory Animals, 8th Edition, and were reviewed and approved by the Institutional Animal Care and Use Committee of the University of Mississippi Medical Center.
All experimental protocols were performed in accordance with the NIH Guide for the Care and Use of Laboratory Animals, 8th Edition, and were reviewed and approved by the Institutional Animal Care and Use Committee of the University of Mississippi Medical Center.
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