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Pectinex ultra mash

Manufactured by Novozymes
Sourced in Denmark, Germany

Pectinex™ Ultra Mash is a laboratory equipment product developed by Novozymes. It is designed to facilitate the breakdown of pectin, a complex polysaccharide found in plant cell walls. The core function of this product is to enable efficient pectin hydrolysis, which is a key step in various industrial processes.

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2 protocols using pectinex ultra mash

1

Optimized Extraction of Pectic RG-I

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The extraction method was optimized to enable industrial scale production and obtain further enrichment of pectic RG-I domains from bell pepper (bpRG-I) and carrot (cRG-I). Commonly used food processing methods were applied including aqueous extraction at 85 °C (bell pepper) or at 45 °C in the presence of Pectinex™ Ultra Mash (Novozymes, Bagsværd, Denmark) food-grade pectinolytic enzymes with pectin lyase and polygalacturonase as main activity (carrot); decanting and filtration to remove non-soluble residues; centrifugation to remove fat (decreaming, bell pepper), ultra- and dia-filtration to remove small molecules (<10 kDa), pasteurization and subsequent spray-drying of the RG-I enriched polysaccharide extracts.
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2

Pectinase Enzyme Preparations for Pectin Hydrolysis

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Pectinase preparations Pectinex ® Ultra SPL (≥3800 U/mL, Sigma-Aldrich, Steinheim, Germany), Pectinex ® BE XXL (13,600 PECTU/g), Pectinex ® Ultra Mash (9500 PECTU/g) and Pectinex ® Ultra Tropical (5000 PECTU/g) from Novozymes (Bagsvaerd, Denmark), and Panzym ® Univers (11,000 PECTU/mL) from Begerow (Langenlonsheim, Germany) were used in this work. Apple pectin, polygalacturonic acid, and galacturonic acid were all purchased from Sigma-Aldrich (Steinheim, Germany). Nylon 6/6 pellets were from Sigma-Aldrich and threads consisting of 60 filaments with a diameter of 25 µm twisted together from Oja, Turkey. All chemical reagents and solvents used were of analytical grade.
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