Anti tfeb antibody a303 673a

Bafilomycin A1 (B1793), carbonyl cyanide m-chlorophenyl hydrazone (CCCP) (C2759), propidium iodide (P4170), oligomycin (O4876), 2-[2-[4(trifluoromethoxy)phenyl]hydrazinylidene]-propanedinitrile (FCCP) (C2920), antimycin A (AA) (A8674), and rotenone (R8875) were purchased from Sigma-Aldrich. SYTO-13 (S7575) was purchased from Thermo Fisher Scientific. The Masson’s Trichrome staining kit was obtained from RAL Diagnostic (RAL-361350-0000). The DeadEnd^TM Fluorometric Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) System was purchased from Promega (G3250). Anti-cleaved-caspase 3 (Asp175) (9664), anti-LC3B (2775), anti-VDAC1 (4661), anti-ATG7 (2631), anti-Beclin 1 (3728), anti-GRP75 (2816), and anti-p-Drp-1 (Ser616) (3455) antibodies were from Cell Signaling Technology. Anti-β-actin (A2228) and anti-SQSTM1/P62 (P0067) were from Sigma-Aldrich. Anti-TOMM 40 antibody (H-300) was from Santa Cruz Biotechnologies. Anti-MOMA 2 antibody (MAB1825) was from Merck Millipore, anti-Parkin (ab15954) and anti-PINK1 (ab74487) were from Abcam. Anti-PGC-1α antibody (NBP1-04676) was from NOVUS Biologicals. Anti-TFEB antibody (A303-673A) was from Bethyl Laboratories. Smooth muscle actin rabbit polyclonal antibody (RB-9010-P) was from Thermo Fisher Scientific. Secondary antibodies conjugated to horseradish peroxidase (HRP) were from Cell Signaling Technology.

Cell sampling and immunoblotting were performed as previously described [8]. Signals were detected with Immunostar^® LD chemiluminescent substrates (Wako) and a LAS‐3000 image analyzer (Fujifilm, Tokyo, Japan). Signal intensity was quantified using multigauge software (Fujifilm). Antibodies against LC3 (PM036), SQSTM1 (PM045), and Lamin B1 (LMNB1) (PM064) were purchased from MBL (Aichi, Japan). Anti‐TFEB antibody (A303‐673A) was purchased from Bethyl Laboratories Inc. (Montgomery, TX, USA). Anti‐NRF2 antibody (sc‐13032) was purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti‐β‐ACTIN (A1978) antibody was purchased from Sigma‐Aldrich. Secondary antibodies included horseradish peroxidase‐conjugated F(ab′)2 fragment of goat anti‐mouse IgG, anti‐rabbit IgG, and anti‐goat IgG (Jackson Immunoresearch, West Grove, PA, USA).