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2 protocols using dl hmtba

1

Methionine Metabolism Pathway Analysis

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Methionine, 5-methylthioadenosine (MTA), cystathionine (Cysta), cysteine (Cys), SAM, homocysteine (Hcy), SAH, GSH, 5-methyltetrahydrofolate (5-MTHF), vitamin B6 (VB6), vitamin B9 (VB9), vitamin B6 (VB12), acetonitrile, formic acid, methanol, ascorbic acid, ammonium acetate, tris-(2-carboxyethyl) phosphine, hydrogen peroxide (H2O2), 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) were supplied by Sigma–Aldrich (St. Louis, USA). Fetal bovine serum, antibiotics (penicillin and streptomycin), and Dulbecco's modified Eagle's medium mixed with Ham's F-12 were provided by Gibco (Shanghai, China). DL–HMTBA was offered by Macklin (Shanghai, China).
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2

Comprehensive Metabolite Quantification Protocol

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Met, SAM, SAH, MTA, Hcy, Cysta, Cys, GSH, 5-MTHF, VB6, VB9, VB12, LC-MS-grade acetonitrile (ACN), LC-grade methanol (MeOH), formic acid, ascorbic acid, sodium hydroxide (NaOH) 0.1 M, hydrochloric acid (HCl), ammonium acetate (NH4OAc), tris-(2-carboxyethyl)phosphine (TCEP), L-Met, and DL-Met were purchased from Sigma-Aldrich (St. Louis, MO, USA). DL-HMTBA was purchased from Macklin (Shanghai, China). DL-MM was purchased from Taopu (Shanghai, China). Homocystine-d8 was purchased from CDN Isotopes (Pointe-Claire, Quebec, Canada). Ultrapure water was obtained from a Millipore-Q water system (Millipore, Bedford, MA, USA). Dulbecco's modified Eagle's medium and Ham's F-12 nutrient mixture (DMEM/F12), RPMI-1640 medium, fetal bovine serum (FBS), and penicillin–streptomycin were purchased from Gibco (Shanghai, China).
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