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Inverted a1r spectral confocal microscope

Manufactured by Nikon
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The Inverted A1r spectral confocal microscope is a high-performance imaging system designed for advanced microscopy applications. It features a confocal optics design that enables high-resolution, optical sectioning, and sensitive fluorescence imaging.

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Lab products found in correlation

Smz1500 microscope, by Nikon (2 mentions) Alexafluor488 conjugated anti chicken secondary antibody, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 conjugated anti rabbit secondary antibody, by Thermo Fisher Scientific (1 mentions) Chicken anti gfp, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 conjugated anti rabbit secondary antibody, by Cell Signaling Technology (1 mentions)

2 protocols using inverted a1r spectral confocal microscope

1

Fluorescent Imaging of Cell Cycle Markers

Cited 1 time
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Images were obtained with a Nikon SMZ1500 microscope or a Nikon inverted A1r spectral confocal microscope. Z stacks were made through the entire CHT during confocal imaging in multiple fluorescent channels. Immunofluorescence double staining was carried out and analyzed as described previously (Gao et al., 2015 (link)), with chicken anti-GFP (1:400, Life Technologies, catalog no. A10262) and rabbit anti-phospho-histone 3 (pH3) antibodies (1:250, abcam, catalog no. ab5176). AlexaFluor488-conjugated anti-chicken secondary antibody (1:1,000, Life Technologies, catalog no A11039) and AlexaFluor594-conjugated anti-rabbit secondary antibody (1:1,000, Life Technologies, catalog no. A11012) were used to reveal primary antibodies.
Mahony C.B., Pasche C, & Bertrand J.Y. (2018). Oncostatin M and Kit-Ligand Control Hematopoietic Stem Cell Fate during Zebrafish Embryogenesis. Stem Cell Reports, 10(6), 1920-1934.
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2

Confocal Imaging of Dorsal Aorta

Cited 1 time
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Images were obtained with a Nikon SMZ1500 microscope or a Nikon inverted A1r spectral confocal microscope. Confocal z-stack image acquisition in multiple fluorescent channels was used to examine the entire dorsal aorta in the AGM . Immunofluorescence double staining was performed as described previously,58 (link) with anti-p-akt S473 (1:25; cat. no. 9271; Cell Signaling) and AlexaFluor 594–conjugated anti-rabbit secondary antibody (1:1000; cat. no. A11012; Life Technologies).
Mahony C.B., Cacialli P., Pasche C., Monteiro R., Savvides S.N, & Bertrand J.Y. (2021). Hapln1b, a central organizer of the ECM, modulates kit signaling to control developmental hematopoiesis in zebrafish. Blood Advances, 5(23), 4935-4948.
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