After enucleation, the lens and cornea were removed and each eye cup incubated in 4% PFA, incubated in a 30% sucrose solution before being frozen in OCT compound (VWR, Lutterworth, UK). Eyes were sectioned and dried before permeabilizing with 0.2% Triton-X 100. Slides were washed in PBS before blocking with 10% bovine serum albumin and 10% donkey serum. Slides were washed before primary antibody incubation (goat polyclonal, ABIN343052, AntibodiesOnline [Aachen Germany], used 1/200 in 1% bovine serum albumin, 1% serum) and secondary antibody incubation (donkey anti-goat 488, ab1050129 [Abcam, Cambridge, UK] used 1/400 in 1% bovine serum albumin and 1% serum). Hoescht stain was performed (Sigma-Aldrich, Gillingham, UK) (1/2,000 in PBS) before a final wash in PBS. ProLong Diamond antifade mounting medium (Life Technologies Ltd, Paisley, UK) was applied and slides were sealed before imaging.
Ab1050129
Manufactured by Abcam
Sourced in United Kingdom
Ab1050129 is a laboratory equipment product. It serves as a core function for scientific research and experimentation purposes. The detailed description of this product is not available due to the requirement to maintain an unbiased and factual approach without extrapolation or interpretation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab1050129
1
Immunohistochemical Analysis of Ocular Tissues
2
Immunohistochemical Analysis of Ocular Tissues
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After enucleation, the lens and cornea were removed and each eye cup incubated in 4% PFA, incubated in a 30% sucrose solution before being frozen in OCT compound (VWR, Lutterworth, UK). Eyes were sectioned and dried before permeabilizing with 0.2% Triton-X 100. Slides were washed in PBS before blocking with 10% bovine serum albumin and 10% donkey serum. Slides were washed before primary antibody incubation (goat polyclonal, ABIN343052, AntibodiesOnline [Aachen Germany], used 1/200 in 1% bovine serum albumin, 1% serum) and secondary antibody incubation (donkey anti-goat 488, ab1050129 [Abcam, Cambridge, UK] used 1/400 in 1% bovine serum albumin and 1% serum). Hoescht stain was performed (Sigma-Aldrich, Gillingham, UK) (1/2,000 in PBS) before a final wash in PBS. ProLong Diamond antifade mounting medium (Life Technologies Ltd, Paisley, UK) was applied and slides were sealed before imaging.
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