The following analytical grade or chemically pure reagents were purchased from commercial sources for this study: 30% hydrogen peroxide (H2O2), sodium acetate trihydrate (CH3CO2Na·3H2O), ferrous sulfate heptahydrate (FeSO4·7H2O), sodium nitrite (NaNO2), disodium hydrogen phosphate dodecahydrate (Na2HPO4·12H2O), sodium dihydrogen phosphate dihydrate (NaH2PO4·2H2O), ferric chloride hexahydrate (FeCl3·6H2O), anhydrous sodium carbonate (Na2CO3), potassium acetate (CH3CO2K), aluminum chloride (AlCl3). Potassium ferricyanide (K3[Fe(CN)6]), salicylic acid sodium, gallic acid monohydrate, Folin-ciocalteu reagent, 2,6-di-tert-butylphenol, Rutin (C27H30O16), t-butyl terephthalate phenol, Tris buffer, 2,4,6-Tri(2-pyridyl)-1,3,5-triazine (TPTA) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were obtained from Sigma chemicals.
2 4 6 tri 2 pyridyl 1 3 5 triazine
Manufactured by Merck Group
Sourced in United States, China, Germany
2,4,6-tri(2-pyridyl)-1,3,5-triazine is a chemical compound used as a lab reagent. It functions as a chelating agent, capable of forming stable complexes with various metal ions.
Automatically generated - may contain errors
Lab products found in correlation
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (6 mentions)
Gallic acid, by Merck Group (5 mentions)
Folin ciocalteu reagent, by Merck Group (4 mentions)
Rutin, by Merck Group (3 mentions)
Sodium acetate, by Merck Group (2 mentions)
Chlorogenic acid, by Merck Group (2 mentions)
Ferulic acid, by Merck Group (2 mentions)
Sodium acetate trihydrate, by Merck Group (2 mentions)
Milli q system, by Merck Group (2 mentions)
Sodium carbonate, by Merck Group (2 mentions)
Butylated hydroxytoluene bht, by Merck Group (2 mentions)
Rutin, by J&K Scientific (2 mentions)
Ascorbic acid, by Merck Group (2 mentions)
2 2 diphenyl 1 picrylhydrazil, by Merck Group (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Absolute ethanol, by Merck Group (2 mentions)
Tetrahydrofuran, by Merck Group (2 mentions)
Superoxide dismutase sod, by Yuanye Bio-Technology (2 mentions)
Xanthine oxidase xod, by Yuanye Bio-Technology (2 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Merck Group (2 mentions)
13 protocols using 2 4 6 tri 2 pyridyl 1 3 5 triazine
1
Analytical Grade Reagents for Research
2
Antioxidant Evaluation of Vaccinium myrtillus Leaves
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Folin–Ciocalteu (F-C) reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-tri (2-pyridyl)-1,3,5-triazine (TPTZ), gallic acid and Trolox were purchased from Sigma-Aldrich (St. Louis, MO, USA). Ethanol, water and methanol HPLC grade, glacial acetic acid, iron chloride hexahydrate and sodium carbonate were purchased from VWR (Radnor, PA, USA). Vanillic acid, quercetin, rutin, chlorogenic acid, ferulic acid and catechin standards were also purchased from Sigma-Aldrich (St. Louis, MO, USA). LC-MS grade methanol and water, acetonitrile, sorbic acid (E-200), 5-fluorouracil and sodium acetate were purchased from Merck KgaA (Darmstadt, Germany).
Vaccinium myrtillus dry leaves were purchased from Bidah Chaumel (Murcia, Spain). They proceed from an organic field located in Huelva (Spain), and were collected in June 2022. Fresh leaves were air dried in dark conditions at room temperature in order to reach a moisture of 10.5%. They were grounded to an average particle size of 0.8 mm and stored at −20 °C until the extraction.
Vaccinium myrtillus dry leaves were purchased from Bidah Chaumel (Murcia, Spain). They proceed from an organic field located in Huelva (Spain), and were collected in June 2022. Fresh leaves were air dried in dark conditions at room temperature in order to reach a moisture of 10.5%. They were grounded to an average particle size of 0.8 mm and stored at −20 °C until the extraction.
3
Comprehensive Microbiological Evaluation Protocol
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Sodium azide and MgSO4 were obtained from Junsei (Japan). Oxgall, MRS agar, MRS broth, tryptone soy agar and broth, and nutrient agar and broth were purchased from Difco (USA). HCl and Na2CO3 were obtained from Daejung (Korea). Agar powder, β-mercapto ethanol, sodium dodecyl sulfate (SDS), σ-nitrophenyl-β-D-galactopyranoside (ONPG), trichlo acetic acid, 2,2-diphenyl-1-picrylhydrazyl, 2, 4, 6-tripyridyl-s-triazine (DPPH), iron (III) chloride hexahydrate 97% A.C.S reagent, 2,4,6-tri(2-pyridyl)-1,3,5-triazine (TPTZ), FeCl3•6H2O, Pyrogallol, K2S2O8, ethylene diamine tetra acetic acid (EDTA), acetate buffer (pH 3.6), Tris-HCl buffer, xylene, sodium acetate trihydrate, and sodium taurodeoxycholate hydrate were obtained from Sigma (USA). A gram stain kit was obtained from YD Diagnostics (Korea). An API 50 CHL kit was obtained from Bio Merieux Ltd. (France). 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid was obtained from Fluka (Germany). Lactic acid was obtained from Konto (Japan). BCP agar was obtained from Aiken (Japan). Skim milk was obtained from Seoul Dairy Co-op. (Korea). Chloroform was obtained from JT Baker (USA). NaOH pellets were obtained from Mallinckrodt (USA). Finally, the sterilized 0.45 μm filters were obtained from Millipore (Ireland).
4
Antioxidant Assays and Phenolic Compound Analysis
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2,2-Diphenyl-1-picrylhydrazyl (DPPH) was purchased from Sigma-Aldrich (Steinheim, Germany). Folin-Ciocalteu reagent, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), 2,4,6-Tri (2′-pyridyl)-1,3,5-triazine (TPTZ) and 3-aminophthalhydrazide, chemical standards in HPLC-grade, such as rutin, gallic acid, p-coumaric acid, chlorogenic acid, caffeic acid and ferulic acid, were purchased from Sigma chemical Co. (St. Louis, MO, USA). Orientin, homoorientin, vitexin and isovitexin standards (HPLC-grade) were obtained from Extrasynthese Company Inc. (Lyon, France). Ultrapure water was prepared by the Milli-Q system (Millipore, Bedford, OH, USA). All solutions prepared for HPLC were passed through 0.45 μm nylon filters before use. All other chemicals were of analytical reagent grade.
5
Urease Activity Quantification Protocol
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Urease from Canavalia ensiformis (Jack bean) (SLBB0100V Sigma Aldrich), thiourea (CAS 62-56-6, Sigma Aldrich), sodium nitroprusside (CAS 13755-38-9, Sigma Aldrich CHEMIE GmbH USA), ceftriaxone (Geltis, Shaigan Pharmaceuticals, Pakistan), sodium carbonate (Merck, USA), 2,4,6-tri(2-pyridyl)-1,3,5-triazine Alfa Aesar, CAS (3682-35-7, Germany) and solvents of analytical grade were purchased from distributor of Sigma Aldrich in Pakistan.
6
Antioxidant Compound Isolation and Analysis
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Rutin was purchased from J&K Scientific Ltd. (Beijing, China), 2,2-diphenyl-1-picrylhdrazyl (DPPH), 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulf onic acid) diammonium salt (ABTS), 2,4,6-Tri(2-pyridyl)-1,3,5-triazine (TPTZ), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), Vc, butylated hydroxytoluene (BHT), and foline-phenol were purchased from Sigma-Aldrich Corp. (Shanghai, China). The purity of the related standards above was equal or greater than 99.5%. The HPLC-grade solvents, such as acetonitrile (ACN), methanol, and formic acid (FA), were obtained from TEDIA Company Inc. (Fairfield, OH, USA). All of the other solvents were purchased from Shanghai Chemical Reagent Corp. (Shanghai, China).
7
Antioxidant Capacity Measurement Protocols
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Folin–Ciocalteu, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-tri-2-pyridyl-1,3,5-triazine (TPTZ), 7,8-tetramethyl-chroman-2-carboxylicacid (Trolox), and ascorbic acid were purchased from Sigma Aldrich (St. Louis, MO, USA). Guaiacol was supplied by Sinopharm Chemical Reagent (Shanghai, China). Acetonitrile of HPLC grade was purchased from Merck(Darmstadt, Germany). N2 (99.999%) gases were purchased from Beijing Beiwen Gas Co., Ltd. (Beijing, China). Other analytical-grade chemicals were provided by Beijing Chemicals Co., Ltd. (Beijing, China).
8
Characterization of Lemon Essential Oil Antioxidant Properties
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Steam-distilled Lemon essential oil was purchased from Green Andina (Bogota, Columbia). Natural waxes were obtained from L&F (Medellín, Columbia). Absolute ethanol (lot SHBK8928), sodium carbonate (Lot 94F-0313), sodium acetate trihydrate (Lot SLBX1773), 2,4,6-tri-(2-pyridyl)-1,3,5-triazine (lot BCBW9015), 2,2-diphenyl-1-picrylhydrazil (lot BCBQ6979), nitroblue tetrazolium (NBT) (lot BCBQ6979), and nicotinamide adenine dinucleotide (NAD) (lot BCBQ6979), were obtained from Sigma-Aldrich (St. Louis, MO, USA). Ferric chloride.6H 2 O (lot 176057) and dimethyl sulfoxide (lot 183521) were purchased from Fisher Chemical (Sunnyvale, CA, USA). In addition, (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox ® ) (97%, lot A0405014) and gallic acid (A0267865) were acquired from Acros Organics (Morris Plains, NJ, USA). Further, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) (lot p31b064) was purchased from Alfa Aesar (Haverhill, MA, USA). Methanol (lot l823009611), Folin-Ciocalteu phenol reagent (lot hc43368401), and tetrahydrofuran (lot dg643), were obtained from Merck (Darmstadt, Germany). 2,2 -Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), 2, 2-diphenyl-1-picrylhydrazil (067k lot 1154), and diammonium salt (lot slbp9592v) were obtained from Sigma-Aldrich (St. Louis, MO, USA).
9
Antimicrobial Activity Evaluation Protocol
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Ethanol and mEthanol were purchased from Molar Chemicals Ltd., Hungary. Folin-Ciocâlteu phenol reagent (2N), sodium carbonate, 2,2-diphenyl-1-picrylhydrazyl, 2,4,6-tri(2pyridyl)-1,3,5-triazine, ascorbic acid, gallic acid, ferric chloride, acetic acid, hydrochloric acid and sodium acetate were obtained from Sigma-Aldrich, Hungary. 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), potassium persulfate, trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid) and LC/MS grade acetonitrile were procured from Merck, Germany. Dimethyl sulfoxide (DMSO) of 99.90% purity was obtained from Sigma-Aldrich, Germany; while Amoxicillin and Penicillin were purchased from Thermo Fisher Scientific, USA. All the chemicals were of analytical grade unless otherwise stated, and used without further purification. Deionized water was used for preparing the reagents and solutions, carrying out extractions and chromatographic analyses.
Standard strains of Staphylococcus aureus (CCM 4223) and Escherichia coli (CCM 3954) were obtained from the Czech collection of microorganisms (Brno, Czech Republic). The strains were cultivated over night at 37 °C on 5% Columbia blood agar (Sigma-Aldrich, Germany).
Standard strains of Staphylococcus aureus (CCM 4223) and Escherichia coli (CCM 3954) were obtained from the Czech collection of microorganisms (Brno, Czech Republic). The strains were cultivated over night at 37 °C on 5% Columbia blood agar (Sigma-Aldrich, Germany).
10
Annatto Seed Extraction and Characterization
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Sodium carbonate (lot a0594092339), anhydrous sodium acetate (lot 882032), absolute ethanol (lot k4958171742), potassium persulphate (lot k40707991048), methanol (lot l823009611), acetic acid glacial (lot k47109963539), acetone (lot k43912814243), sodium hydroxide (lot b0895598319), concentrated hydrochloric acid (lot k41017317016), Folin–Ciocalteu phenol reagent (lot hc43368401), Mueller–Hinton broth (lot b1223098542), tetrahydrofuran (lot dg643), and 2,4,6-tri-(2-pyridyl)-1,3,5-triazine (lot l58155738107) were obtained from Merck (Darmstadt, Germany). Dimethyl sulfoxide (lot 190260) was obtained from PanReac AppliChem (Barcelona, Spain). 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), 2, 2-diphenyl-1-picrylhydrazil (067k lot 1154), and diammonium salt (lot slbp9592v) were obtained from Sigma-Aldrich (St. Louis, MO, USA). (±)-6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) (97% lot stbb6668) was obtained from Aldrich Chem (St. Louis, MO, USA). Ferric chloride. 6H2O (lot 9n005099n) was obtained from Carlo Erba (Barcelona, Spain). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (lot p31b064) was obtained from Alfa Aesar (Haverhill, MA, USA). Annatto seeds were purchased from a farmers market of Medellín city (Colombia), which were sun-dried until reaching a moisture content of 10.58 ± 0.98%.
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