Mmp 9

Manufactured by GE Healthcare

Sourced in United Kingdom

The MMP-9 is a lab equipment product used for the detection and quantification of the Matrix Metalloproteinase-9 (MMP-9) enzyme. MMP-9 is involved in the breakdown of extracellular matrix proteins and plays a role in various physiological and pathological processes. The MMP-9 product provides a tool for researchers and clinicians to measure MMP-9 levels in samples, which can be useful for studying disease mechanisms, biomarker development, and therapeutic monitoring.

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Lab products found in correlation

Tnf α, by R&D Systems (1 mentions) Human il 6, by R&D Systems (1 mentions) Biotrack activity assay kit, by GE Healthcare (1 mentions) Mmp 2, by GE Healthcare (1 mentions)

2 protocols using mmp 9

Biomarker Measurement in Western Populations

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Biomarkers were selected based on a previous study7 (link) and have been used in Western population studies.26 (link)
human IL-6, IL-8, TNF-α, and matrix metalloproteinase (MMP)-9 levels were measured by commercial enzyme-linked immunosorbent assay kits. The minimum detection limits of human IL-6 (R&D Systems), IL-8 (R&D Systems), TNF-α (R&D Systems), and MMP-9 (GE Healthcare, Amersham, Buckinghamshire, UK) were 2.0, 31.3, 15.6, and 1,000 pg/mL, respectively. A half value limit of detection for each biomarker was used for statistical analysis when levels were not detected.

Kawayama T., Kinoshita T., Matsunaga K., Kobayashi A., Hayamizu T., Johnson M, & Hoshino T. (2016). Responsiveness of blood and sputum inflammatory cells in Japanese COPD patients, non-COPD smoking controls, and non-COPD nonsmoking controls. International Journal of Chronic Obstructive Pulmonary Disease, 11, 295-303.

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Quantitative Gelatinase Activity Assay

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The MMP2 and MMP9 enzymatic activity was further quantitated using gelatinase substrate activity as previously described [16 (link)] using MMP-2(RPN2631), MMP-9 (RPN2634), biotrack activity assay kit (GE Healthcare, Pittsburgh, PA) as per manufacturer protocols. The concentration of active MMP was interpolated from a standard curve obtained using the manufacturer provided standard.

Platt D., Amara S., Mehta T., Vercuyssee K., Myles E.L., Johnson T, & Tiriveedhi V. (2014). Violacein inhibits matrix metalloproteinase mediated CXCR4 expression: Potential anti-tumor effect in cancer invasion and metastasis. Biochemical and biophysical research communications, 455(0), 107-112.

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