Turbomass clarus 500 gc ms gc fid

Chemical analysis of Z. lotus samples was performed by a TurboMass Clarus 500 GC-MS/GC-FID from Perkin Elmer instruments (Waltham, MA, USA) equipped with a Stabilwax fused-silica capillary column (Restek, Bellefonte, PA, USA) (60 m × 0.25 mm, 0.25 µm film thickness). The operating conditions used were as follows: GC oven temperature was kept at 60 °C for 5 min and programmed to 220 °C at a rate of 5 °C/min, and kept constant at 220 °C for 25 min. Helium was used as a carrier gas at a flow rate of 1 mL/min. Solvent delay was 0–2 min and scan time was 0.2 s. Mass range was from 30 to 350 m/z using electron-impact at 70 eV mode. An amount of ~2 µg of each Z. lotus extract was diluted in 1 mL of methanol and 1 μL of the solution was injected into the GC injector at a temperature of 280 °C. The analysis was repeated twice. Relative percentages for quantification of the components were calculated by electronic integration of the GC-FID peak areas. The identification of the constituents was made by comparing the obtained mass spectra for each component with those reported in mass spectra Nist and Willey libraries. Linear retention indices (LRI) of each compound were calculated using a mixture of aliphatic hydrocarbons (C8–C30, Ultrasci) injected directly into GC injector at the same temperature program reported above.

The gas chromatographic/mass spectrometric (GC/MS) analysis was carried out with a GC-MS and GC-FID using a Turbomass Clarus 500 GC-MS/GC-FID from Perkin Elmer instruments (Waltham, MA, USA). A Stabilwax fused-silica capillary column (Restek, Bellefonte, PA, USA) (60 m × 0.25 mm, 0.25 μm film thickness) was used with helium as the carrier gas (1.0 mL/min). The GC oven temperature was kept at 60 °C for 5 min and programmed to 220 °C at a rate of 5 °C/min, and kept constant at 220 °C for 30 min. MS was taken at 70 eV. The mass range was from 30 to 350 m/z. 1 μL of oil was diluted in 1 mL of methanol and 1 μL of the solution was injected into the GC injector at the temperature of 280 °C [25 (link),29 (link)].
The identification of the constituents was made by comparison of the obtained mass spectra for each compound with those reported in mass spectra NIST, NBS, and Wiley Libraries. Linear retention indices (LRIs) were calculated using a mixture of aliphatic hydrocarbons (C8-C30, Ultrasci), injected directly into the GC injector at the same conditions reported above. Relative percentages for quantification of the separated components were calculated by an electronic integration of the GC-FID peak areas using the normalization method without the use of correction factors. All analyses were repeated twice.