Antibodies used in this study included: UBR5 (#8755, CST®, 1:1000); anti-mouse-BrdU (Becton Dickinson, different dilutions); anti-rat-BrdU (ab6326, Abcam; 1:500); PCNA-PC10 (Cancer Research, UK, 1:1000); Vimentin (V6389, Sigma-Aldrich, 1:1000); RNF8 (ab15850, Abcam; 1:2000); RNF168 (#ABE367, Millipore; 1:500); Tubulin (clone B-5-1-2, Sigma-Aldrich); Anti-Flag M2 (F3165, Sigma-Aldrich); 53BP1 (NB100-305, Novusbio, 1:2000); Chk1-PhosphoS345 (#2341 CST®,1:1000); Chk1 (#2360 CST®,1:1000); Chk2-PhosphoT68 (#2661 CST®, 1:500); Chk2(#2662 CST®, 1:1000); TRIP12 (ab86220, Abcam; 1:500); Histone H2A(ab18255, Abcam; 1:1000); polη (custom made, raised against the peptide: VQVEQRQNPHLRNKPC, 1:1000); polη-PhosphoS601(Eurogentec,(15 (link))); Histone H2A.X-PhosphoS139 (Upstate), RPA2 (1:1000, Millipore).
Ab15850
Manufactured by Abcam
Sourced in United Kingdom, United States
Ab15850 is a primary antibody product offered by Abcam. It is a polyclonal antibody raised in rabbit against a specific target. The core function of this product is to detect and bind to its target antigen for research applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab18255, by Abcam (1 mentions)
Ab6326, by Abcam (1 mentions)
Rat anti brdu, by Abcam (1 mentions)
Rnf168, by Merck Group (1 mentions)
Histone h2a, by Abcam (1 mentions)
Vimentin, by Merck Group (1 mentions)
Mouse anti brdu, by BD (1 mentions)
Anti flag m2, by Merck Group (1 mentions)
Ab1791, by Abcam (1 mentions)
Sc 7392, by Santa Cruz Biotechnology (1 mentions)
Sc 22760, by Santa Cruz Biotechnology (1 mentions)
Sc 6954, by Santa Cruz Biotechnology (1 mentions)
Sc 55538, by Santa Cruz Biotechnology (1 mentions)
Sc 5286, by Santa Cruz Biotechnology (1 mentions)
2 protocols using ab15850
1
Antibody Characterization Protocol for DNA Damage Response
2
Western Blot and Immunoprecipitation Antibodies
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The following antibodies were used for western blot analysis: anti-MDC1 rabbit polyclonal antibody (R2, manufactured in our lab) [1 (link)], anti-KPNA2 mouse monoclonal antibody (sc-55538, Santa cruz, Dallas, TX, USA), anti-HA mouse monoclonal antibody (sc-7392, Santa Cruz), anti-GFP mouse monoclonal antibody (sc-5286, Santa Cruz), anti-Histone3 rabbit polyclonal antibody (ab1791, Abcam, Cambridge, UK), and anti-α-Tubulin mouse monoclonal antibody (sc-5286, Santa Cruz). For immunoprecipitation assay, anti-HA rabbit polyclonal antibody (F-7, Santa Cruz), anti-GFP mouse monoclonal antibody (sc-5286, Santa Cruz), anti-MDC1 rabbit polyclonal antibody (R2, manufactured in our lab), and anti-KPNA2 mouse monoclonal antibody (sc-55538, Santa cruz) were used. The following antibodies were used for immunofluorescence staining: anti-MDC1 rabbit polyclonal antibody (R2), anti-HA mouse monoclonal antibody (sc-7392, Santa Cruz), anti-RNF8 goat polyclonal antibody (ab15850, Abcam), anti-53BP1 rabbit polyclonal antibody (sc-22760, Santa Cruz), anti-BRCA1 mouse monoclonal antibody (sc-6954, Santa cruz), anti-γH2AX mouse monoclonal antibody (05-636-1, Millipore, Burlington, MA, USA), and anti-RNF168 rabbit polyclonal antibody (ABE367, Millipore).
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