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Alexa fluor 647 donkey anti rabbit igg h l antibody

Manufactured by Thermo Fisher Scientific
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Alexa Fluor® 647 Donkey Anti-Rabbit IgG (H+L) Antibody is a secondary antibody conjugated with Alexa Fluor® 647 dye. It is designed to detect and bind to rabbit primary antibodies.

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Lab products found in correlation

Abscisic acid, by Merck Group (2 mentions) 24 well μ plate, by Ibidi (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) Epr4166 2, by Abcam (1 mentions) Ab2865, by Abcam (1 mentions) Pa1 981, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 goat anti rabbit igg h l antibody, by Thermo Fisher Scientific (1 mentions) Anti dsp, by Santa Cruz Biotechnology (1 mentions) A1 confocal, by Leica (1 mentions) Anti cd63, by Santa Cruz Biotechnology (1 mentions) Dmi6000b, by Leica (1 mentions)

Close spelling variants of this product

Alexa Fluor 647 donkey anti-rabbit IgG (H+L) Anti-rabbit IgG H&L Alexa Fluor®647 Alexa Fluor 647 donkey anti-rabbit IgG Alexa Fluor 647 donkey anti-rabbit Fluor® 647 donkey anti-rabbit IgG Donkey anti-rabbit IgG-alexa 647 Donkey anti-rabbit IgG (H + L) Alexa Fluor 647 anti-rabbit IgG Alexa Fluor® donkey anti-rabbit IgG Alexa Fluor IgG H + L

6 protocols using alexa fluor 647 donkey anti rabbit igg h l antibody

1

Fluorescence Microscopy of NF-κB Translocation

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Translocation of NF‐kB to the nucleus was evaluated by fluorescence microscopy. Briefly, after 24 h treatment, PBMC were washed in PBS and fixed with −20°C methanol for 20 min. Then, cells were washed, incubated with 0.1% Triton X‐100 for 10 min, blocked with 1% BSA for 1 h, and incubated with rabbit polyclonal anti‐NF‐kB p65 (phosphor S536, Abcam, Cambridge, UK) as primary antibody and Alexa Fluor 647‐donkey anti‐rabbit IgG (H+L) antibody (Life Technologies, Carlsbad, CA) as secondary antibody at room temperature. The slides were extensively washed with PBS and viewed with a fluorescence microscope. DAPI was used as nuclei‐specific dye.
Russo R., Barsanti L., Evangelista V., Frassanito A.M., Longo V., Pucci L., Penno G, & Gualtieri P. (2016). Euglena gracilis paramylon activates human lymphocytes by upregulating pro‐inflammatory factors. Food Science & Nutrition, 5(2), 205-214.
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2

Chromatin Immunoprecipitation for Epigenetic Markers

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U2OS cells stably expressing CRISPR-EChO and sgRNA were seeded at 10% confluence in 24-well μ-plate (Ibidi 82406) and treated with 100 μM abscisic acid (Sigma A1049) or DMSO (Sigma D2650) vehicle for 2 days. Cells were fixed in 4% paraformaldehyde (ThermoFisher 28906) for 15 m, then rinsed 3 times with DPBS (Life Technologies 14190-250) for 5 m each. Fixed cells were blocked in 5% normal donkey serum (Jackson ImmunoResearch Laboratories 017-000-121) and 0.3% Triton X-100 (ThermoFisher 85111) in DPBS for 1 h at room temperature, then incubated overnight in at 4°C with primary antibodies. Samples were rinsed 3 times with DPBS for 5 m each, then incubated with secondary antibody 2 h at room temperature in the dark with agitation. Samples were rinsed 3 times with DPBS for 5 m each, then stored in DPBS for imaging.
Primary antibodies used in this study are Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (Abeam ab8898) used at 1:500 dilution, Anti-CBX1 / HP1 beta antibody (Abeam ab10478) used at 1:200 dilution, and Anti-KAP1 antibody - ChIP Grade (Abcam ab10483) used at 1:500 dilution. The secondary antibody used in this study is Alexa Fluor® 647 Donkey Anti-Rabbit IgG (H+L) Antibody (Life Technologies A31573) used at 1:500 dilution.
Gao Y., Han M., Shang S., Wang H, & Qi L.S. (2021). Interrogation of the Dynamic Properties of Higher-Order Heterochromatin Using CRISPR/dCas9. Molecular cell, 81(20), 4287-4299.e5.
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3

CRISPR-EChO Assay for Chromatin Modifications

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U2OS cells stably expressing CRISPR-EChO and sgRNA were seeded at 10% confluence in 24well μ-plate (Ibidi 82406) and treated with 100 μM abscisic acid (Sigma A1049) or DMSO (Sigma D2650) vehicle for 2 days. Cells were fixed in 4% paraformaldehyde (ThermoFisher 28906) for 15 m, then rinsed 3 times with DPBS (Life Technologies 14190-250) for 5 m each. Fixed cells were blocked in 5% normal donkey serum (Jackson ImmunoResearch Laboratories 017-000-121) and 0.3% Triton X-100 (ThermoFisher 85111) in DPBS for 1 h at room temperature, then incubated overnight in at 4°C with primary antibodies. Samples were rinsed 3 times with DPBS for 5 m each, then incubated with secondary antibody 2 h at room temperature in the dark with agitation. Samples were rinsed 3 times with DPBS for 5 m each, then stored in DPBS for imaging. Primary antibodies used in this study are Anti-Histone H3 (tri methyl K9) antibody -ChIP Grade (Abcam ab8898) used at 1:500 dilution, Anti-CBX1 / HP1 beta antibody (Abcam ab10478) used at 1:200 dilution, and Anti-KAP1 antibody -ChIP Grade (Abcam ab10483) used at 1:500 dilution. The secondary antibody used in this study is Alexa Fluor® 647 Donkey Anti-Rabbit IgG (H+L) Antibody (Life Technologies A31573) used at 1:500 dilution.
Gao Y., Han M., Shang S., Wang H., & Qi L.S. (2021). Interrogation of the Dynamic Properties of Higher-Order Heterochromatin Using CRISPR/dCas9.
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4

Immunophenotyping of Human Skin Samples

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4 mm punch biopsy specimens of human skin were formalin fixed and paraffin embedded as previously described (91 (link)). Primary antibodies used were: anti-human CD4 monoclonal antibody (Invitrogen, N1UG0), anti-human IL-17A biotinylated antibody (R&D Systems, BAF317), anti-human CD69 antibody (Novus Biologicals, 8B6), anti-human CD103 (Abcam, EPR4166-2), and recombinant anti-Ki67 (Abcam, SP6). Secondary antibodies used were: Alexa Fluor 555 rabbit anti-PE polyclonal antibody (VWR), Alexa Fluor 647 donkey anti-Rabbit IgG (H+L) antibody (Invitrogen, A21147), Alexa Fluor 555 goat anti-mouse IgG2a antibody (Invitrogen, A-21242), Alexa Fluor 633 goat anti-mouse IgG1 polyclonal antibody (Invitrogen), Alexa Fluor 488 goat anti-rabbit IgG (H+L) polyclonal antibody (Invitrogen). Slides were imaged with a Mantra Quantitative Pathology Imaging microscope (PerkinElmer) by the Human Skin Disease Resource Center at Brigham & Women’s Hospital, Harvard Medical School.
Whitley S.K., Li M., Kashem S.W., Hirai T., Igyártó B.Z., Knizner K., Ho J., Ferris L.K., Weaver C.T., Cua D.J., McGeachy M.J, & Kaplan D.H. (2022). Local IL-23 is required for proliferation and retention of skin-resident memory Th17 cells. Science immunology, 7(77), eabq3254.
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5

Fluorescent Immunolabeling of Cellular Markers

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Anti-pericentriolar material 1 (PCM1) antibody (rabbit polyclonal, Atlas Antibodies, #HPA023370, dilution 1:200), anti-Myc antibody (rabbit polyclonal, Invitrogen, #PA1-981, dilution 2μg), and anti-phospholamban (PLN) antibody [2D12] (mouse monoclonal, Abcam, #ab2865, dilution 1:100) were used. The following fluorophore-conjugated secondary antibodies were used: Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L) Antibody, 1:400 (Invitrogen), Alexa Fluor 555 Goat Anti-Mouse IgG (H+L) Antibody, 1:400 (Invitrogen), Alexa Fluor 647 Donkey Anti-Mouse IgG (H+L) Antibody, 1:400 (Invitrogen), and Alexa Fluor 647 Donkey Anti-Rabbit IgG (H+L) Antibody, 1:400 (Invitrogen).
Bhattacharyya S., Sathe A.A., Bhakta M., Xing C, & Munshi N.V. (2019). PAN-INTACT enables direct isolation of lineage-specific nuclei from fibrous tissues. PLoS ONE, 14(4), e0214677.
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6

Immunofluorescence Analysis of CD63, DSP, and Amelogenin

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Tissue sections were incubated with anti-CD63 (1:200, Santa Cruz Biotechnology, Santa Cruz, CA, USA). Tissue sections from Rab27aash/ash mutant mice were incubated with anti-DSP (1:100, Santa Cruz) and anit-amelogenin (1:200, Santa Cruz). Secondary antibodies, Alexa Fluor 647 donkey anti-rabbit IgG (H+L) antibody (1:2000, Invitrogen) or Alexa Fluor 488 donkey anti-goat IgG (H+L) antibody (1:2000, Invitrogen), were applied for 60 min at room temperature. Samples were sealed with Vecta shield mounting medium containing DAPI. Images were taken by using a Nikon A1 Confocal or Leica DMI6000B.
Jiang N., Xiang L., He L., Yang G., Zheng J., Wang C., Zhang Y., Wang S., Zhou Y., Sheu T.J., Wu J., Chen K., Coelho P.G., Tovar N.M., Kim S.H., Chen M., Zhou Y.H, & Mao J.J. (2017). Exosomes Mediate Epithelium–Mesenchyme Crosstalk in Organ Development. ACS nano, 11(8), 7736-7746.
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