The gold coated slides (20 nm Au deposited on 8 mm×8 mm glass slide by Physical Vapor Deposition (Kurt Lesker PVD75 E-Beam Evaporator System) were first cleaned with O2 plasma (South Bay Technologies PC2000 Plasma Cleaner) for 15 minutes. The slides were then incubated in a mixture of 1 mM 11-mercaptodecanoic acid (HSC10COOH, Aldrich) and 8-mercapto-octanol (HSC8OH, Aldrich) in an absolute ethanol solution (ACROS Organics) with 1:5 mole ratio overnight to form a self-assembled monolayer (SAM) by alkyl thiols. After SAM formation, the gold slides were incubated in a 0.5 mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC, TCI)/ N-hydroxysuccinimide (NHS, Aldrich) for 2 hours to activate the carboxylic acid groups.26 (link), 27 (link) Next, the gold slides were rinsed with deionized water and immediately moved to a freshly prepared 3 mL solution containing 0.3 mg/mL of CNDs for 2 hours. The gold slides were rinsed with deionized water and dried before experiments.
Absolute ethanol solution
Manufactured by Thermo Fisher Scientific
Absolute ethanol solution is a laboratory-grade solvent consisting of 100% ethanol. It is a clear, colorless liquid that is commonly used in various scientific applications as a general-purpose solvent, reagent, and disinfectant.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using absolute ethanol solution
1
Carboxylated Carbon Nanoparticle Immobilization
2
Functionalization of Gold Surfaces with CRY1
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The gold coated slides contained 20 nm of Au deposited
on a 11.5 mm × 11.5 mm glass slide (surface area of 1.32 cm2) by physical vapor deposition (Kurt Lesker PVD75 E-Beam Evaporator
System). Gold slides were first cleaned with O2 plasma
(South Bay Technologies PC2000 Plasma Cleaner) for 3 min. The slides
were then incubated in a mixture of 11-mercaptoundecanoic acid (HS(CH2)10COOH, Sigma-Aldrich) and 8-mercapto-octanol
(HS(CH2)8OH, Sigma-Aldrich) in an absolute ethanol
solution (ACROS Organics) with a 1:2 mole ratio overnight to form
a SAM. After SAM formation, the gold slides were incubated in a 10
mM PBS solution with 0.5 mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide
hydrochloride (EDC, TCI)/N-hydroxysuccinimide (NHS,
Sigma-Aldrich) for 2 h to activate the carboxylic acid groups. Next,
the gold slide was rinsed with 10 mM PBS solution and immediately
moved to a freshly prepared 10 mM PBS solution containing 1 mg/mL
of CRY1 for 2 h in a black box. This was followed by dipping 10 min
in a 0.2 M glycine PBS solution to deactivate the remaining active
sites on the SAM. The gold slides were rinsed with 10 mM PBS solution
and then used for electrochemical measurement.
on a 11.5 mm × 11.5 mm glass slide (surface area of 1.32 cm2) by physical vapor deposition (Kurt Lesker PVD75 E-Beam Evaporator
System). Gold slides were first cleaned with O2 plasma
(South Bay Technologies PC2000 Plasma Cleaner) for 3 min. The slides
were then incubated in a mixture of 11-mercaptoundecanoic acid (HS(CH2)10COOH, Sigma-Aldrich) and 8-mercapto-octanol
(HS(CH2)8OH, Sigma-Aldrich) in an absolute ethanol
solution (ACROS Organics) with a 1:2 mole ratio overnight to form
a SAM. After SAM formation, the gold slides were incubated in a 10
mM PBS solution with 0.5 mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide
hydrochloride (EDC, TCI)/N-hydroxysuccinimide (NHS,
Sigma-Aldrich) for 2 h to activate the carboxylic acid groups. Next,
the gold slide was rinsed with 10 mM PBS solution and immediately
moved to a freshly prepared 10 mM PBS solution containing 1 mg/mL
of CRY1 for 2 h in a black box. This was followed by dipping 10 min
in a 0.2 M glycine PBS solution to deactivate the remaining active
sites on the SAM. The gold slides were rinsed with 10 mM PBS solution
and then used for electrochemical measurement.
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