Rabbit polyclonal anti phospho erk1 2

Minocycline, doxycycline, U0126 and BAY11-7082 were purchased from Sigma Chemical Company (St. Louis, MO, USA). SB203580 was purchased from Wako Industrial Company (Osaka, Japan). Tigecycline was a gift from Pfizer Inc. (New York, NY, USA). LPS from Pseudomonas aeruginosa Stereotype 10 (Sigma Chemical Company) was used as a bacterial component that induces cytokine and chemokine production. Tetracyclines were dissolved in nanopure water and stored at −20 °C. LPS was dissolved in nanopure water and stored at −80 °C. Primary antibodies included rabbit polyclonal anti-IκBα and phospho-IκBα, rabbit polyclonal anti-IKKα and phospho-IKKα, rabbit polyclonal anti-IKKβ and phospho-IKKβ, rabbit polyclonal anti-NF-κB and phospho-NF-κB, rabbit polyclonal anti-phospho-ERK1/2, rabbit polyclonal anti-phospho-p38 (all from Cell Signaling Technology, Beverly, MA, USA), and rabbit polyclonal anti-actin antibody (Sigma Chemical Company).