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Anti atg13

Manufactured by Cell Signaling Technology
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Anti-ATG13 is a laboratory reagent produced by Cell Signaling Technology. It is an antibody that recognizes the ATG13 protein, which is involved in the regulation of autophagy, a cellular process. The antibody can be used for the detection and analysis of ATG13 in various experimental systems.

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Lab products found in correlation

Anti ulk1, by Cell Signaling Technology (3 mentions) P 4e bp1 thr37 46, by Cell Signaling Technology (2 mentions) Anti p mtor ser2448, by Cell Signaling Technology (2 mentions) Anti lc3b wb nb600 1384, by Novus Biologicals (2 mentions) Anti lamp1, by Thermo Fisher Scientific (2 mentions) Anti p62 hpa003196, by Merck Group (2 mentions) Anti yh2ax, by Cell Signaling Technology (2 mentions) Anti cd11b pe, by Agilent Technologies (2 mentions) Anti tfeb, by Cell Signaling Technology (2 mentions) Anti fasn, by Cell Signaling Technology (2 mentions) Anti α tubulin, by Cell Signaling Technology (2 mentions) Anti cleaved parp, by Cell Signaling Technology (2 mentions) Nigericin, by Merck Group (1 mentions) Amiodarone hydrochloride, by Merck Group (1 mentions) Lidocaine hydrochloride monohydrate, by Merck Group (1 mentions) Betahistine dihydrochloride, by Merck Group (1 mentions) Procainamide hydrochloride, by Merck Group (1 mentions) Hydroxychloroquine, by Merck Group (1 mentions) Chloroquine, by Merck Group (1 mentions) Monensin, by Merck Group (1 mentions)

Close spelling variants of this product

Rabbit anti-ATG13 ATG13

6 protocols using anti atg13

1

Autophagy Protein Detection and Inhibition

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The following primary antibodies were used at the indicated dilutions: anti-LC3A/B (1:1000 for western blotting and 1:100 for immunofluorescence; Cell Signaling Technology, 4108), anti-LC3A (1:100 for immunohistofluorescence; Abgent, 1805a), anti-ATG12 (1:100; Cell Signaling Technology, 2010), anti-ATG13 (1:1000; Cell Signaling Technology, 6940), anti-ATG16L1 (1:1000; Cell Signaling Technology, 8089), anti-human LAMP1 and anti-mouse LAMP1 (1:100; Becton Dickinson, 555798 and 553792), anti-WIPI2 (1:100; Bio-Rad, MCA5780GA), anti-CDSN/corneodesmosin (R&D Systems, AF5725), anti-GAPDH (1:2000; Santa Cruz Biotechnology, sc-25778), and anti-ATP6V0D1 (1:50; ABCAM, ab56441). The following inhibitors and reagents were used: Amiodarone hydrochloride (Sigma, A8423), bafilomycin A1 (Tocris Biosciences, 1334), betahistine dihydrochloride (Sigma, B4638), chloroquine (Sigma, C6628), CCCP (Sigma, C2759), HBSS (Gibco, 14025–092), hydroxychloroquine (Sigma, H0915), lidocaine (Sigma, L7757), lidocaine hydrochloride monohydrate (Sigma, L5647), monensin (Sigma, M5273), NH4Cl (Sigma, 213330), nigericin (Sigma, N7143), PP242 (Tocris, 4257), procainamide hydrochloride (Sigma, P9391). Mito-TMRE (abcam, 113852), PIK3C3/VPS34 inhibitor (IN-1) was kindly provided by Dr I. Ganley, University of Dundee.
Jacquin E., Leclerc-Mercier S., Judon C., Blanchard E., Fraitag S, & Florey O. (2017). Pharmacological modulators of autophagy activate a parallel noncanonical pathway driving unconventional LC3 lipidation. Autophagy, 13(5), 854-867.
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2

Western Blotting Antibodies Protocol

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Anti-protein A (P-3775; Sigma-Aldrich), anti-GFP (11814460001; Roche), anti-Tor1 (sc-11900; Santa Cruz Biotechnology), anti-PGK (459250; Thermo Fisher Scientific), rabbit anti-Goat IgG HRP (anti-TAP) (ab6741; abcam), goat anti-mouse IgG, human ads-HRP (1030–05; Southern Bio Tech), anti-rabbit IgG HRP-linked antibody (7074S; Cell signaling), anti-Atg13 (a gift from Dr. Yoshinori Ohsumi, Tokyo Institute of Technology), and anti-HA (901501; BioLegend) antibodies were used for western blotting.
Ukai H., Araki Y., Kira S., Oikawa Y., May A.I, & Noda T. (2018). Gtr/Ego-independent TORC1 activation is achieved through a glutamine-sensitive interaction with Pib2 on the vacuolar membrane. PLoS Genetics, 14(4), e1007334.
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3

Comprehensive Antibody-based Proteomics Profiling

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Antibodies used were anti-FASN (3180; Cell Signaling, Switzerland), anti-LC3B (WB: NB600-1384, Novus biological, Switzerland; IF: 3868; Cell Signaling, Switzerland) anti-LAMP1 (14-1079-80; Thermofisher, Switzerland), anti-p62 (HPA003196; Sigma-Aldrich, Switzerland), anti-TFEB (4240; Cell Signaling, Switzerland), anti-p-TFEB (Ser 211) (37681S, Cell Signaling, Switzerland), anti-p-AKT (Ser473) (4060S, Cell Signaling, Switzerland) anti-PTEN (9552, Cell Signaling, Switzerland), anti-ULK1 (4776; Cell Signaling, Switzerland), anti-p-ULK1 (Ser757, equivalent to Ser758 of human ULK1) (6888; Cell Signaling, Switzerland), anti-ATG13 (6940; Cell Signaling, Switzerland), anti-pATG13 (Ser318) (600-401-C49; Rockland, Switzerland), anti p-mTOR (Ser2448) (5536; Cell Signaling, Switzerland), p4E-BP1 (Thr37/46) (2855; Cell Signaling, Switzerland), anti-α-tubulin (3873; Cell Signaling, Switzerland), anti-cleaved PARP (9541; Cell Signaling, Switzerland), anti yH2AX (2577; Cell Signaling, Switzerland) and anti-CD11b-PE (R0841; Dako, Switzerland).
Humbert M., Seiler K., Mosimann S., Rentsch V., Sharma K., Pandey A.V., McKenna S.L, & Tschan M.P. (2021). Reducing FASN expression sensitizes acute myeloid leukemia cells to differentiation therapy. Cell Death and Differentiation, 28(8), 2465-2481.
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4

Signaling Pathway Inhibitors and Autophagy Markers

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LPS (#L2880), SB230580 (#S8307), Anisomycin (#A5862), SP600125 (#S5567), and MG132 (#474790) were purchased from Sigma-Aldrich. LY3214996 (#HY100494), Bafilomycin A1 (#HY-100558), and Cycloheximide (#HY-12320) were purchased from MedChem Express. The following primary antibodies were used: anti-p38 MAPK (#9217, Cell Signaling), antiphospho-p38 MAPK (#9216, Cell Signaling), anti-NLRP3 (#15101, Cell Signaling), anti-IL1 (#12703, Cell Signaling), anti-Atg13 (#13273, Cell Signaling), anti-LC3 (#8899, Cell Signaling), anti-p62 (#5114 Cell Signaling), anti-ULK1 (#8054, Cell Signaling), anti-FIP200 (#12436, Cell Signaling), anti-ubiquitin (#134953, Abcam), anti-Atg5 (#9980, Cell Signaling), anti-Myc (#2276, Cell Signaling), anti-GST (#2625, Cell Signaling), and β-actin as a loading control (#4970, Cell Signaling).
Wu Y., He Y., Wang F., Yao N., Zhao Y, & Tian Z. (2022). Lipopolysaccharide Inhibits Autophagy and Promotes Inflammatory Responses via p38 MAPK-Induced Proteasomal Degradation of Atg13 in Hepatic Stellate Cells. Mediators of Inflammation, 2022, 9603989.
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5

Autophagy Regulatory Pathway Analysis

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Antibodies used were anti-FASN (3180; Cell Signaling, Switzerland), anti-LC3B (WB: NB600-1384, Novus biological, Switzerland; IF: 3868; Cell Signaling, Switzerland) anti-LAMP1 (14-1079-80; Thermofisher, Switzerland), anti-p62 (HPA003196 ; Sigma-Aldrich, Switzerland), anti-TFEB (4240; Cell Signaling, Switzerland) anti-ULK1(4776; Cell Signaling, Switzerland), anti p-ULK1 (Ser757) (6888; Cell Signaling, Switzerland), anti-ATG13 (6940 ; Cell Signaling, Switzerland), anti-pATG13 (Ser318) (600-401-C49; Rockland, Switzerland), anti p-mTOR (Ser2448) (5536; Cell Signaling, Switzerland), p4E-BP1 (Thr37/46) (2855; Cell Signaling, Switzerland), anti-α-tubulin (3873; Cell Signaling, Switzerland), anti-cleaved PARP (9541; Cell Signaling, Switzerland), anti yH2AX (2577; Cell Signaling, Switzerland) and anti-CD11b-PE (R0841; Dako, Switzerland).
Humbert M., Seiler K., Mosimann S., Rentsch V., Marfè G., & Tschan M.P. (2020). Reducing FASN expression sensitizes acute myeloid leukemia cells to differentiation therapy.
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6

Comprehensive Autophagy Signaling Antibody Panel

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The following primary antibodies were used in this study: anti-AMPK antibody (#2532S; Cell Signaling Technology), anti-p-AMPK antibody (#2535S; Cell Signaling Technology), anti-LC3 I/II (#4108S; Cell Signaling Technology), anti-P62/SQSTM1 (#8025S; Cell Signaling Technology), anti-Beclin1 (ab62557; Abcam), anti-NRAGE (sc-136552; Santa Cruz Biotechnology), anti-GAPDH (#5174; Cell Signaling Technology), anti-Ulk1 (#2707773; Millipore), anti-p-Ulk1 (Ser758; #2571270; Millipore), anti-ATG13 (#13468S; Cell Signaling Technology), anti-p-ATG13 (S355; #43533S; Cell Signaling Technology), anti-PI3k (#4263S; Cell Signaling Technology), and anti-mTOR (#2643610; Millipore). Secondary antibodies used for western blotting were as follows: 800CW goat anti-mouse and 800CW goat antirabbit, purchased from LI-COR Biosciences. Hydroxychloroquine (HCQ), purchased from Sigma, was diluted in phosphate-buffered saline (PBS, pH 8.0) at a stock concentration 5 M.
Zhou Y., Huang N., Wu J., Zhen N., Li N., Li Y, & Li Y.X. (2017). Silencing of NRAGE induces autophagy via AMPK/Ulk1/Atg13 signaling pathway in NSCLC cells. Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 39(6).
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