Antimycin a a8674

Manufactured by Merck Group

Sourced in United States

Antimycin A (A8674) is a laboratory product manufactured by Merck Group. It is a complex organic compound that functions as a respiratory inhibitor. This product is primarily used in research applications to investigate cellular respiration and mitochondrial processes.

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Lab products found in correlation

Mitotracker red, by Thermo Fisher Scientific (3 mentions) Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (2 mentions) Ammonium iron 2 sulfate hexahydrate, by Merck Group (1 mentions) Rotenone r8875, by Merck Group (1 mentions) Glutamine, by Thermo Fisher Scientific (1 mentions) Resveratrol, by Fujifilm (1 mentions) Resveratrol, by ChromaDex (1 mentions) Anti ubiquitin, by Cell Signaling Technology (1 mentions) Hoechst 33342, by Fujifilm (1 mentions) Viafect transfection reagent, by Promega (1 mentions) Anti total 4e bp1, by Cell Signaling Technology (1 mentions) Anti lc3a b, by Cell Signaling Technology (1 mentions) Anti p62 gp62 c, by Progen Biotechnik (1 mentions) Rneasy fibrous tissue mini kit, by Qiagen (1 mentions) Human umbilical vein endothelial cells huvecs, by American Type Culture Collection (1 mentions) Mitosox red, by Thermo Fisher Scientific (1 mentions)

4 protocols using antimycin a a8674

Ferroptosis Induction Assay

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Hydrogen peroxide (H₂O₂) solution (216763), RSL3 (SML2234), erastin (E7781), menadione (M5625), rotenone (R8875), antimycin A (A8674), and ammonium iron (II) sulfate hexahydrate (09719) were purchased from Sigma-Aldrich. For the in vitro cell experiments, the compounds and HC were added to the assay medium simultaneously (DMEM supplemented with 1% FBS).

Hwang J.W., Park J.H., Park B.W., Kim H., Kim J.J., Sim W.S., Mishchenko N.P., Fedoreyev S.A., Vasileva E.A., Ban K., Park H.J, & Baek S.H. (2021). Histochrome Attenuates Myocardial Ischemia-Reperfusion Injury by Inhibiting Ferroptosis-Induced Cardiomyocyte Death. Antioxidants, 10(10), 1624.

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Rotenone and Antimycin A Cell Culture

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Rotenone (R8875) and antimycin A (A8674), were purchased from Sigma-Aldrich (St Louis, MO, USA). Dulbecco/Vogt modified Eagle’s minimal essential medium (DMEM) (D5030, Gibco), glutamine (25030149, Gibco), and glucose (A2494001, Gibco) were purchased from ThermoFisher Scientific (Carlsbad, CA, USA).

Kluza J., Peugnet V., Daunou B., Laine W., Kervoaze G., Rémy G., Loyens A., Maboudou P., Fovez Q., Grangette C., Wolowczuk I., Gosset P., Garçon G., Marchetti P., Pinet F., Pichavant M, & Dubois-Deruy E. (2021). A New Strategy to Preserve and Assess Oxygen Consumption in Murine Tissues. International Journal of Molecular Sciences, 23(1), 109.

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Resveratrol and Autophagy Regulation Assay

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Resveratrol (185-01721) and Hoechst 33342 (346-07951) were from Wako Pure Chemicals (Osaka, Japan). Food grade Resveratrol for mouse treatment was from ChromaDex (ASB-00018089-101, Irvine, CA). FITC-conjugated wheat germ agglutinin (WGA) lectin (W834), dihydroethidium (DHE) (D1168), MitoSOX Red (M36008), MitoTracker Red (MTR, M7512), and Lipofectamine RNAiMAX Transfection Reagent (13778-150) were from Thermo Fisher Scientific (Rockford, IL). The RNeasy Fibrous Tissue Mini Kit (74704) was from Qiagen (Valencia, CA). The GoScript Reverse Transcription System (A6010), GoTaq qPCR Master Mix (A600A), and ViaFect Transfection Reagent (E4982) were from Promega (Madison, WI). Antimycin A (A8674) and chloroquine (CQ) (C6628) were from Sigma-Aldrich (St. Louis, MO). Plasmid EGFP-LC3 Expression Vector was from Addgene (#11546). siRNAs against mouse Atg5 (SASI_Mm01_00089196), mouse Pink1 (SASI_Mm02_00331134), and MISSION siRNA Universal Negative Control (SIC-001) were from Sigma Genosys Japan (Ishikari, Japan). Antibodies used were as follows: anti-LC3AB (#12741), anti-phosho-Ser65-4EBP1 (#9451), anti-total 4EBP1 (#9452), and anti-ubiquitin (#3936) from Cell Signaling Technology (Beverly, MA), anti-p62 (GP62-C) from Progen (Heidelberg, Germany), and anti-GAPDH (G8795) and anti-α-tubulin (T5168) from Sigma-Aldrich.

Sebori R., Kuno A., Hosoda R., Hayashi T, & Horio Y. (2018). Resveratrol Decreases Oxidative Stress by Restoring Mitophagy and Improves the Pathophysiology of Dystrophin-Deficient mdx Mice. Oxidative Medicine and Cellular Longevity, 2018, 9179270.

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Mitochondrial Respiration Assay in HUVEC

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OIigomycin (O4876), FCCP (C2920), rotenone (R8875), antimycin A (A8674), and M199 (M4530) were purchased from Sigma Aldrich (St. Louis, MO). DMEM (10566-016), fetal bovine serum (FBS) (16000044), antimycotic and antibiotic (15240062), and MitoSOX Red (M36008) were purchased from Thermo Fisher Scientific (Waltham, MA). Human umbilical vein endothelial cells (HUVECs) were purchased from ATCC (Manassas, VA).

Deer E., Vaka V.R., McMaster K.M., Wallace K., Cornelius D.C., Amaral L.M., Cunningham M.W, & LaMarca B. (2020). Vascular endothelial mitochondrial oxidative stress in response to preeclampsia: a role for angiotension II type 1 autoantibodies. American journal of obstetrics & gynecology MFM, 3(1), 100275.

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