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Histone h3 acetyl k27

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Histone H3 (acetyl K27) is a lab equipment product that plays a core role in the study of epigenetic modifications. It is a highly specific antibody that recognizes histone H3 acetylated at lysine 27, a post-translational modification associated with active gene transcription.

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Lab products found in correlation

Phospho stat3 tyr705, by Cell Signaling Technology (2 mentions) Horseradish peroxidase hrp, by GE Healthcare (2 mentions) Total p53, by Cell Signaling Technology (2 mentions) Supersignal west femto kit, by Thermo Fisher Scientific (2 mentions) P44 42 mapk erk1 2, by Cell Signaling Technology (2 mentions) Acetyl p53 lys379, by Cell Signaling Technology (2 mentions) Stat3 79d7, by Cell Signaling Technology (2 mentions) Phospho p44 42 mapk erk1 2 thr202 tyr204, by Cell Signaling Technology (2 mentions) Pvdf membrane, by Merck Group (2 mentions) Histone h3, by Abcam (2 mentions) β actin, by Abcam (2 mentions) Mouse anti e cadherin, by Cell Signaling Technology (1 mentions) Histone h3 acetyl k9, by Abcam (1 mentions) Histone h3 acetyl k18, by Abcam (1 mentions) Hdac1, by Cell Signaling Technology (1 mentions) Horseradish peroxidase conjugated secondary antibody, by Merck Group (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Vimentin, by Cell Signaling Technology (1 mentions)

3 protocols using histone h3 acetyl k27

1

Western Blot Analysis of Cellular Proteins

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Whole cell lysates were prepared from 7 x 105 cells in 2 x SDS-DTT sample buffer. Lysates were run on SDS-PAGE gels and transferred to PVDF membranes (Millipore) using standard protocols. Membranes were probed with the following primary antibodies at 1:1000 dilution unless otherwise stated: Acetyl-p53 Lys379 (1:500) (2570; Cell Signalling), total p53 (1C12; Cell Signalling), Phospho-p44/42 MAPK (Erk1/2) Thr202/Tyr204 (9101; Cell Signalling), p44/42 MAPK (Erk1/2) (9102; Cell Signalling), Phospho-Stat3 (Tyr705) (9131; Cell Signaling), Stat3 (79D7) (4904; Cell Signalling), Histone H3 (acetyl K27) (ab4729, Abcam), Histone H3 (ab1791, Abcam), Beta Actin (ab8227, Abcam). Membranes were probed with secondary antibodies conjugated to horseradish peroxidase (HRP) (GE Healthcare; 1:5000) and proteins detected using SuperSignal West Femto kit (Thermo Scientific).
Horton S.J., Giotopoulos G., Yun H., Vohra S., Sheppard O., Bashford-Rogers R., Rashid M., Clipson A., Chan W.I., Sasca D., Yiangou L., Osaki H., Basheer F., Gallipoli P., Burrows N., Erdem A., Sybirna A., Foerster S., Zhao W., Sustic T., Harrison A.P., Laurenti E., Okosun J., Hodson D., Wright P., Smith K.G., Maxwell P., Fitzgibbon J., Du M.Q., Adams D.J, & Huntly B.J. (2017). Early loss of Crebbp confers malignant stem cell properties on lymphoid progenitors. Nature cell biology, 19(9), 1093-1104.
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2

Western Blot Antibody Validation Protocol

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The procedures for Western blot have been described previously.10 (link) The mouse anti-E-cadherin (1/1000), rabbit anti-DAXX (1/1000), vimentin (1/500), GAPDH (1/5000) and HDAC-1 (1/2000) were from Cell Signaling Technologies (Danvers, MA). The rabbit anti-histone H3 (acetyl K14) (1/2000), histone H3 (acetyl K27) (1/1000), histone H3 (acetyl K9) (1/10000), histone H3 (acetyl K18) (1/1000) and histone H3 (acetyl K23) (1/1000) were from Abcam. Horseradish peroxidase-conjugated secondary antibodies (anti-rabbit IgG: 1:5000, or anti-mouse IgG: 1:5000) were from Sigma (St. Louis, MO).
Wu C., Ding H., Wang S., Li Y., Liu S.B., Wang X., Zheng J., Xue T., Amin H.M., Song Y.H, & Zhou J. (2020). DAXX inhibits cancer stemness and epithelial–mesenchymal transition in gastric cancer. British Journal of Cancer, 122(10), 1477-1485.
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3

Western Blot Analysis of Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole cell lysates were prepared from 7 x 105 cells in 2 x SDS-DTT sample buffer. Lysates were run on SDS-PAGE gels and transferred to PVDF membranes (Millipore) using standard protocols. Membranes were probed with the following primary antibodies at 1:1000 dilution unless otherwise stated: Acetyl-p53 Lys379 (1:500) (2570; Cell Signalling), total p53 (1C12; Cell Signalling), Phospho-p44/42 MAPK (Erk1/2) Thr202/Tyr204 (9101; Cell Signalling), p44/42 MAPK (Erk1/2) (9102; Cell Signalling), Phospho-Stat3 (Tyr705) (9131; Cell Signaling), Stat3 (79D7) (4904; Cell Signalling), Histone H3 (acetyl K27) (ab4729, Abcam), Histone H3 (ab1791, Abcam), Beta Actin (ab8227, Abcam). Membranes were probed with secondary antibodies conjugated to horseradish peroxidase (HRP) (GE Healthcare; 1:5000) and proteins detected using SuperSignal West Femto kit (Thermo Scientific).
Horton S.J., Giotopoulos G., Yun H., Vohra S., Sheppard O., Bashford-Rogers R., Rashid M., Clipson A., Chan W.I., Sasca D., Yiangou L., Osaki H., Basheer F., Gallipoli P., Burrows N., Erdem A., Sybirna A., Foerster S., Zhao W., Sustic T., Harrison A.P., Laurenti E., Okosun J., Hodson D., Wright P., Smith K.G., Maxwell P., Fitzgibbon J., Du M.Q., Adams D.J, & Huntly B.J. (2017). Early loss of Crebbp confers malignant stem cell properties on lymphoid progenitors. Nature cell biology, 19(9), 1093-1104.
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