Mouse anti human mlh1 clone g168 15

Manufactured by Biocare Medical

Mouse anti-human MLH1 (clone G168-15) is a monoclonal antibody that recognizes the human MLH1 protein. MLH1 is a key component of the DNA mismatch repair system and plays a crucial role in maintaining genomic stability.

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Lab products found in correlation

Anti human msh2 clone fe11, by Biocare Medical (2 mentions) Anti human msh6 clone bc 44, by Biocare Medical (2 mentions)

Close spelling variants of this product

MLH1 (clone G168-15,

2 protocols using mouse anti human mlh1 clone g168 15

Immunohistochemical Assessment of MMR Protein Expression

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MMR protein (MLH1, MSH2 and MSH6) expression was analyzed in formalin-fixed, paraffin-embedded tumor sections using an immunoperoxidase method (27 (link)). Monoclonal antibodies included mouse anti-human MLH1 (clone G168-15, Biocare Medical, Concord, CA), anti-human MSH2 (clone FE11, Biocare Medical, Concord, CA), and anti-human MSH6 (clone BC/44; Biocare Medical, Concord, CA). MMR protein loss was defined as the absence of nuclear staining in tumor cells in the presence of positive nuclear staining in normal colonic epithelium and lymphocytes. Tumors were classified as MMR-deficient (vs MMR-proficient) if loss of one or more MMR proteins was detected.

Yoon H.H., Tougeron D., Shi Q., Alberts S.R., Mahoney M.R., Nelson G.D., Nair S.G., Thibodeau S.N., Goldberg R.M., Sargent D.J, & Sinicrope F.A. (2014). KRAS codon 12 and 13 mutations in relation to disease-free survival in BRAF-wild type stage III colon cancers from an adjuvant chemotherapy trial (N0147 Alliance). Clinical cancer research : an official journal of the American Association for Cancer Research, 20(11), 3033-3043.

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Immunohistochemical Analysis of MMR Proteins

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MMR protein (MLH1, MSH2 and MSH6) expression was analyzed in formalin-fixed, paraffin-embedded tumor sections as previously described (19 (link)). Monoclonal antibodies included mouse anti-human MLH1 (clone G168-15, Biocare Medical, Concord, CA), anti-human MSH2 (clone FE11, Biocare), and anti-human MSH6 (clone BC/44; Biocare). MMR protein loss was defined as the absence of nuclear staining in tumor cells in the presence of nuclear staining in normal colonic epithelium and lymphocytes. Tumors were categorized as deficient (d) vs proficient (p) MMR if loss of at least one MMR protein was detected.

Sinicrope F.A., Mahoney M.R., Yoon H.H., Smyrk T.C., Thibodeau S.N., Goldberg R.M., Nelson G.D., Sargent D.J, & Alberts S.R. (2015). Analysis of Molecular Markers by Anatomic Tumor Site in Stage III Colon Carcinomas from Adjuvant Chemotherapy Trial NCCTG N0147 (Alliance). Clinical cancer research : an official journal of the American Association for Cancer Research, 21(23), 5294-5304.

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