Guanidine hydrochloride

Manufactured by AppliChem

Sourced in Germany

Guanidine hydrochloride is a chemical compound commonly used in laboratory settings. It is a colorless, crystalline solid that is highly soluble in water. Guanidine hydrochloride is a chaotropic agent, which means it can disrupt the hydrogen bonding in water, leading to the denaturation of proteins. This property makes it a useful tool in various biochemical and molecular biology applications.

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Lab products found in correlation

4 protocols using guanidine hydrochloride

HPV16 E7 Antigen Conjugation to Nanoparticles

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NPs were synthesized, functionalized and characterized as previously described (26 (link)). For antigen conjugation, the HPV16 E7 long peptide or the full length E7 protein were dissolved in DMSO and incubated for 12h in endotoxin-free water in the presence of NPs and guanidine hydrochloride (AppliChem) at room temperature. NP-E7LP was purified by size-exclusion chromatography using CL-6B matrix (Sigma-Aldrich), eluted and stored in PBS at room temperature. The size of NP before and after conjugation was determined by dynamic light scattering and remained around 30 nm. E7LP loading on the nanoparticles was measured by BCA assay (Thermo Fisher Scientific). NPs alone have been shown to have no adjuvant activity.

Galliverti G., Wullschleger S., Tichet M., Muruga D., Zangge N., Horton W., Korman A., Coussens L.M., Swartz M.A, & Hanahan D. (2019). Myeloid Cells Orchestrate Systemic Immunosuppression, Impairing the Efficacy of Immunotherapy against HPV + Cancers. Cancer immunology research, 8(1), 131-145.

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Synthesis of Polymeric Biocide

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The following chemicals were used without further purification for the synthesis of the polymeric biocide: guanidine hydrochloride, 98% (Applichem, Darmstadt, Germany), hexamethylenediamine (98%), 2-naphtalenesulphonic acid, sodium salt (technical grade, Sigma-Aldrich, Taufkirchen, Germany), and ethanol (95%). Rilsan^®PA11 (granules) was supplied by Arkema (King of Prussia, PA, USA).

Moshynets O., Bardeau J.F., Tarasyuk O., Makhno S., Cherniavska T., Dzhuzha O., Potters G, & Rogalsky S. (2019). Antibiofilm Activity of Polyamide 11 Modified with Thermally Stable Polymeric Biocide Polyhexamethylene Guanidine 2-Naphtalenesulfonate. International Journal of Molecular Sciences, 20(2), 348.

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Preparation of Pharmaceutical Standards

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Native human AAG (lot no. SLBJ6840V), carvedilol, (+)-cis-diltiazem hydrochloride, dipyridamole, vinblastine sulfate, (±)-propranolol hydrochloride, imipramine hydrochloride, lidocaine, and DMSO were purchased from Sigma-Aldrich (St. Louis, MO, USA). All standards had a specified purity of ≥97% and have been used without further purification. Sodium phosphate dihydrate and sodium hydroxide were purchased from Kemika (Zagreb, Croatia). Tween^® 20 was purchased from Fischer Scientific (Pittsburgh, PA, USA). Guanidine hydrochloride was purchased from AppliChem GmbH (Darmstadt, Germany). Ultrapure water was obtained using SG Ultra Clear UV Plus (SG, Burladingen, Germany). All other chemicals used in the study were of analytical grade or higher purity.

Šeba T., Kerep R., Weitner T., Šoić D., Keser T., Lauc G, & Gabričević M. (2024). Influence of Desialylation on the Drug Binding Affinity of Human Alpha-1-Acid Glycoprotein Assessed by Microscale Thermophoresis. Pharmaceutics, 16(2), 230.

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NMR Protein Sample Preparation

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EDTA was purchased from Merck (Whitehouse station, U.S.A.), guanidine hydrochloride (ultrapure) from AppliChem (Darmstadt, Germany) and bis-tris hydrochloride from Sigma (St. Louis, U.S.A.). Urea (ultra pure) was bought from MP Biomedicals (Illkirch, France). ¹⁵N-NH₄Cl, ¹³C-glucose and D₂O was purchased from Cambridge Isotope Laboratories (Andover, MA, USA). ZnSO₄ was from Fluka (Buchs, Switzerland). Spectra/Por dialysis membranes were from Spectrum Laboratories (Rancho Dominguez, U.S.A.)

Szpryngiel S., Oliveberg M, & Mäler L. (2015). Diffuse binding of Zn2+ to the denatured ensemble of Cu/Zn superoxide dismutase 1. FEBS Open Bio, 5, 56-63.

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