Bio plex human cytokine 21 plex panel

Serum and CSF cytokine profiles were analyzed using Bio-Plex (Bio-Rad, Hercules, CA, USA) multiplex magnetic bead-based antibody detection kits following the manufacturer’s instructions. Bio-Plex Pro Human Cytokine 27-plex Panel and Bio-Plex Human Cytokine 21-plex Panel were used for detection for a total of 48 analytes. Serum and CSF aliquots (50 µl) were used for analysis, with a minimum of 50 beads per analyte acquired. Median fluorescence intensities were measured using a Luminex 200 analyzer. Data collected were analyzed with MasterPlex CT control software and MasterPlex QT analysis software (Hitachi Software, San Bruno, CA, USA). Standard curves for each analyte were generated using standards provided by the manufacturer.

Serum cytokine levels were analyzed using Bio-Plex (Bio-Rad, Hercules, CA, USA) multiplex magnetic bead-based antibody detection kits following the manufacturer's instructions. Bio-Plex Pro Human Th17 Cytokine Panel, Bio-Plex Pro Human Cytokine 27-plex Panel, and Bio-Plex Human Cytokine 21-plex Panel were used for detection of a total of 57 analytes. Serum aliquots (50 μL) were collected from healthy donors and 42 MS cases. A minimum of 50 beads per analyte was acquired. Median fluorescence intensities were measured using a Luminex 200 analyzer. Data collected was analyzed with MasterPlex CT control software and MasterPlex QT analysis software (Hitachi Software San Bruno, CA, USA). Standard curves for each analyte were generated using standards provided by manufacturer.

Serum cytokine levels were analyzed using Bio-Plex (Bio-Rad, Hercules, CA, USA) multiplex magnetic bead-based antibody detection kits following manufacturer's instructions. Multiplex kits, Bio-Plex Pro Human Cytokine 27-Plex Panel (IL-1β, IL-1Ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12(p70), IL-13, IL-15, IL-17А, CCL2, CCL3, CCL4, CCL5, CCL11, CXCL10, TNF-α, G-CSF, GM-CSF, IFN-γ, PDGF-BB, and VEGF) and Bio-Plex Human Cytokine 21-Plex Panel (IL-1α, IL-2Ra, IL-3, IL-12(p40), IL-16, IL-18, CCL7, CCL27, CXCL1, CXCL9, CXCL12, HGF, IFN- α2, LIF, M-CSF, MIF, β-NGF, SCF, SCGF-β, TNF-β, and TRAIL), were used for detection of a total of 48 analytes. Serum aliquots analyzed were 50 μL. A minimum of 50 beads per analyte was acquired. Median fluorescence intensities were collected using Luminex 100 or 200 analyzer (Luminex, Austin, TX, USA). Data collected was analyzed using MasterPlex CT control software and MasterPlex QT analysis software (MiraiBio, San Bruno, CA, USA). Standard curves for each analyte were generated using standards provided by manufacturer.