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Alexa fluor 488 or alexa fluor 594 labelled secondary antibody

Manufactured by Thermo Fisher Scientific
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Alexa Fluor 488 or Alexa Fluor 594-labelled secondary antibodies are fluorescently-tagged antibodies used to detect and visualize target proteins in various experimental techniques, such as immunofluorescence and flow cytometry. These secondary antibodies bind to the primary antibody that is specific to the target protein of interest, allowing for the detection and localization of the target within a sample.

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Lab products found in correlation

Mounting medium, by Agilent Technologies (2 mentions) H 140, by Santa Cruz Biotechnology (2 mentions) Sc 393325, by Santa Cruz Biotechnology (2 mentions) Ec plan neofluar 40x 1.3 oil, by Zeiss (2 mentions)

Close spelling variants of this product

Alexa Fluor 488 or 594 secondary antibodies Alexa Fluor 594-labelled secondary antibody Alexa Fluor 488-labelled secondary antibody Alexa 488-labelled secondary antibody Alexa-fluor-labelled secondary antibodies Alexa Fluor 594 secondary antibody Alexa Fluor 488 secondary antibody Alexa 488 or 594 Alexa-labelled secondary antibody Alexa Fluor 488 antibody

2 protocols using alexa fluor 488 or alexa fluor 594 labelled secondary antibody

1

Immunofluorescence Staining of Tfr2 and Osterix

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For immunofluorescence staining, cells were grown on glass slides. At the desired time point, cells were fixed with 100% methanol for 15 min, permeabilized with 0.5% Triton X-100 for 10 min and after washing for three times, blocked with 1% BSA in PBS for 30 min. Afterwards, cells were incubated with an anti-mouse Tfr2 antibody (H-140, Santa Cruz) over night at 4 °C. After washing, cells were stained with an anti-mouse osterix antibody (sc-393325, Santa Cruz) or phalloidin at RT for 1 h. Subsequently, cells were washed and incubated for 1 h with an Alexa Fluor 488 or Alexa Fluor 594-labelled secondary antibody (Life Technologies), washed, and stained with DAPI for 5 min. After washing again, glass slides were embedded in a small droplet of mounting medium (Dako). Slides were examined using a Zeiss LSM 510 confocal microscope (Zeiss EC Plan-Neofluar 40x/1.3 Oil), and photographs were taken and processed with the Zen 2009 software.
Rauner M., Baschant U., Roetto A., Pellegrino R.M., Rother S., Salbach-Hirsch J., Weidner H., Hintze V., Campbell G., Petzold A., Lemaitre R., Henry I., Bellido T., Theurl I., Altamura S., Colucci S., Muckenthaler M.U., Schett G., Komla Ebri D., Bassett J.H., Williams G.R., Platzbecker U, & Hofbauer L.C. (2019). Transferrin receptor 2 controls bone mass and pathological bone formation via BMP and Wnt signaling. Nature metabolism, 1(1), 111-124.
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2

Immunofluorescence Staining of Tfr2 and Osterix

Check if the same lab product or an alternative is used in the 5 most similar protocols
For immunofluorescence staining, cells were grown on glass slides. At the desired time point, cells were fixed with 100% methanol for 15 min, permeabilized with 0.5% Triton X-100 for 10 min and after washing for three times, blocked with 1% BSA in PBS for 30 min. Afterwards, cells were incubated with an anti-mouse Tfr2 antibody (H-140, Santa Cruz) over night at 4 °C. After washing, cells were stained with an anti-mouse osterix antibody (sc-393325, Santa Cruz) or phalloidin at RT for 1 h. Subsequently, cells were washed and incubated for 1 h with an Alexa Fluor 488 or Alexa Fluor 594-labelled secondary antibody (Life Technologies), washed, and stained with DAPI for 5 min. After washing again, glass slides were embedded in a small droplet of mounting medium (Dako). Slides were examined using a Zeiss LSM 510 confocal microscope (Zeiss EC Plan-Neofluar 40x/1.3 Oil), and photographs were taken and processed with the Zen 2009 software.
Rauner M., Baschant U., Roetto A., Pellegrino R.M., Rother S., Salbach-Hirsch J., Weidner H., Hintze V., Campbell G., Petzold A., Lemaitre R., Henry I., Bellido T., Theurl I., Altamura S., Colucci S., Muckenthaler M.U., Schett G., Komla Ebri D., Bassett J.H., Williams G.R., Platzbecker U, & Hofbauer L.C. (2019). Transferrin receptor 2 controls bone mass and pathological bone formation via BMP and Wnt signaling. Nature metabolism, 1(1), 111-124.
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