Catalase activity assay kit

The coagonist of GCGR and GLP-1R, Aib2 C24 Chimera2 (H¹SQGT⁵FTSDY¹⁰SKYLD¹⁵EQAAK²⁰EFIAW²⁵LMNT-NH²) was synthesized at Zydus Research Centre, Ahmedabad, India[20 (link)]. Kits for triglycerides, cholesterol, and glucose were purchased from Avantor Performance Materials India Ltd, India. ELISA kits for IL-6 and TNF-α were obtained from BD Biosciences, United States; Insulin from Crystal Chem, United States; FGF21 from Wuhan Eiaab science, Co., Ltd. China; leptin and Adiponectin were purchased from B-bridge international, United States. Superoxide dismutase (SOD) and catalase activity assay kit were obtained from Cayman Chemical, United States. Creatinine, blood urea nitrogen, and albumin assay kit were obtained from Sigma-Aldrich, United States. TRIzol reagent and cDNA reverse transcription kit purchased from Invitrogen, Life Technology, United States and QIAGEN Quanti Fast SYBR Green kit were purchased from Qiagen, Germantown, United States. All other chemicals and reagents were purchased from Sigma-Aldrich chemicals, United States unless stated otherwise.

The activities of catalase, SOD, and GPx were measured using catalase activity assay kit (Cayman Chemical, Ann Arbor, MI, USA), superoxide dismutase activity assay kit (Cayman Chemical), and glutathione peroxidase activity kit (Abcam), respectively. NHDF cells were seeded in 100-mm dishes at a density of 5 × 10⁵ cells. Cells were pretreated or not pretreated with each conditioned medium (1×) for 24 h, followed by exposure to 600 μM H₂O₂ for 2 h. The reaction was processed according to the manufacturer’s protocol. Activities of catalase, superoxide dismutase, and glutathione peroxidase were measured at 540, 440, and 340 nm, respectively.