Mouse monoclonal anti flag m2 peroxidase

Antibodies used were mouse monoclonal anti-HA (12CA5, Sigma Aldrich, St. Louis, MO, USA), mouse monoclonal anti-GFP (B-2, Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse monoclonal anti-DLG1 (2D11, Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse monoclonal anti-Scrib (C-6, Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse monoclonal anti-FLAG-M2-peroxidase (Sigma Aldrich, St. Louis, MO, USA), mouse monoclonal anti-18E6 (BF7, Santa Cruz Biotechnology, Santa Cruz, CA, USA), rabbit monoclonal anti-CDK2 (D12, Santa Cruz Biotechnology, Santa Cruz, CA, USA), mouse monoclonal anti- α tubulin (GTU-88, Sigma Aldrich, St. Louis, MO, USA) and mouse monoclonal anti- β-Gal (Promega, Madison, USA). Secondary anti-rabbit HRP and anti-mouse HRP antibodies were obtained from Dako.

All antibodies were from commercial sources: HA—horseradish peroxidase (anti-HA-HRP), 3F10 (Roche, Basel, Switzerland), rabbit monoclonal anti-MST2 (Abcam), goat polyclonal anti-MST2 (C-19; Santa Cruz, CA, USA), rabbit polyclonal p-T180-MST2 (Cell Signaling, Danvers, MA, USA), goat polyclonal anti-LATS1 (n-18 and g-16; Santa Cruz), rabbit monoclonal p-T1079-LATS1, rabbit polyclonal anti-YAP1 (Santa Cruz), mouse monoclonal anti-YAP1 (Sigma, Dallas, TX, USA), rabbit polyclonal p-S127-YAP (Cell Signaling), mouse monoclonal anti-IQGAP1 (MBL), rabbit polyclonal anti-IQGAP1 (Santa Cruz), mouse monoclonal C-Myc tag (Santa Cruz), AKT, p-S308-AKT, p-S473-AKT (Cell Signaling), mouse monoclonal anti-Tubulin and rabbit monoclonal anti-GAPDH (Santa Cruz), mouse monoclonal anti-FLAG M2-Peroxidase (Sigma), rabbit monoclonal anti-GFP, mouse monoclonal anti-TEF-1 (BD Biosciences), mouse monoclonal anti-p-T183/Y185-ERK1/2 and rabbit polyclonal anti-ERK1/2 (Sigma). Mouse monoclonal anti-GFP (Roche), rabbit anti-GFP or goat antiserum (Sigma-Aldrich, St Louis, MO, USA) were used as isotopic IgG immunoprecipitation control.

Equal amounts of samples in 1 × Laemmli buffer were electrophoresed on an 8–16% Tris-Glycine gel (Life Technologies), transferred to nitrocellulose membrane, blotted with the indicated antibodies, and visualized by ECL plus kit (GE Healthcare) using LAS 3,000 imager (Fuji Film). The antibodies used in IF and WB were as followings: Rabbit polyclonal rabbit anti-DAXX (F7810, 1:1,000 dilution), mouse monoclonal anti-beta-Actin-Peroxidase (A33854, 1:2,000 dilution), mouse monoclonal anti-FLAG M2-Peroxidase (A8592, 1:2,000 dilution), and anti-FLAG M2 Affinity Gel (A2220) were purchased from Sigma-Aldrich. Mouse monoclonal anti-EAD (MAB8186, 1:500 dilution), anti-EBV VCA (MAB8184, 1:500 dilution), and rabbit polyclonal anti-Histone H3 (06-755, 1:1,000 dilution) were purchased from EMD Millipore. Rabbit polyclonal anti-GAPDH (ab9485, 1:1,000 dilution) was purchased from Abcam. Mouse monoclonal anti-EBNA1 (MCA2707, 1:600 dilution) was purchased from BioRad. Mouse monoclonal anti-HA (12CA5, 1:1,000 dilution) was from Roche. Rabbit anti-BNRF1 (1:100 dilution) and anti-BALF2 (1:200 dilution) were raised against the peptides from BNRF1 and BALF2, and custom produced by YenZym Antibodies, LLC.