Discovery ultra automated ihc ish staining instrument

Manufactured by Roche

The Discovery ULTRA automated IHC/ISH staining instrument is a laboratory equipment designed for performing immunohistochemistry (IHC) and in situ hybridization (ISH) staining procedures. It automates the staining process to ensure consistent, high-quality results.

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Lab products found in correlation

Mers cov nucleocapsid protein rabbit antibody, by Sino Biological (3 mentions) Tissue tek vip 6, by Sakura Finetek (2 mentions) Discovery chromomap dab, by Roche (1 mentions) Vip 6 tissue tektissue processor, by Sakura Finetek (1 mentions) Discovery chromomap dab kit, by Roche (1 mentions)

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Discovery Ultra (119 citations) Discovery ULTRA automated staining instrument (4 citations) Discovery ULTRA automated (4 citations) Discovery Ultra instrument (4 citations) Automated IHC instrument (2 citations)

3 protocols using discovery ultra automated ihc ish staining instrument

MERS-CoV Lung Tissue Histopathology

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Necropsies and tissue sampling were performed according to IBC-approved protocols. Lungs were perfused with 10% formalin and processed for histologic review. Harvested tissues were fixed for a minimum of 7 days in 10% neutral-buffered formalin and then embedded in paraffin. Tissues were processed using a VIP-6 Tissue-Tek (Sakura Finetek, USA) tissue processor and embedded in Ultraffin paraffin polymer (Cancer Diagnostics, Durham, NC). Samples were sectioned at 5 μm, and the resulting slides were stained with hematoxylin and eosin. Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000. The tissues were processed for immunohistochemistry using the Discovery ULTRA automated IHC/ISH staining instrument (Ventana Medical Systems) with a Discovery Red (Ventana Medical Systems) kit. All tissue slides were evaluated by a board-certified veterinary anatomic pathologist blinded to study group allocations.

van Doremalen N., Haddock E., Feldmann F., Meade-White K., Bushmaker T., Fischer R.J., Okumura A., Hanley P.W., Saturday G., Edwards N.J., Clark M.H., Lambe T., Gilbert S.C, & Munster V.J. (2020). A single dose of ChAdOx1 MERS provides protective immunity in rhesus macaques. Science Advances, 6(24), eaba8399.

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Histological Lung Analysis of MERS-CoV Infection

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Necropsies and tissue sampling were performed according to IBC-approved protocols. Lungs were perfused with 10% formalin and processed for histologic review. Harvested tissues were fixed for a minimum of seven days in 10% neutral-buffered formalin and then embedded in paraffin. Tissues were processed using a VIP-6 Tissue Tek, (Sakura Finetek, USA) tissue processor and embedded in Ultraffin paraffin polymer (Cancer Diagnostics, Durham, NC). Samples were sectioned at 5 μm, and resulting slides were stained with hematoxylin and eosin. Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000. The tissues were processed for immunohistochemistry using the Discovery ULTRA automated IHC/ISH staining instrument (Ventana Medical Systems) with a Discovery ChromoMap DAB (Ventana Medical Systems) kit, scanned with the Aperio ScanScope AT2 (Aperio Technologies, Inc.) and the entire section analyzed with the ImageScope Positive Pixel Count algorithm (version 9.1). All tissue slides were evaluated by a board-certified veterinary anatomic pathologist.

van Doremalen N., Letko M., Fischer R.J., Bushmaker T., Yinda C.K., Schulz J., Seifert S.N., Kim N.J., Hemida M.G., Kayali G., Park W.B., Perera R.A., Tamin A., Thornburg N.J., Tong S., Queen K., van Kerkhove M.D., Choi Y.K., Oh M.D., Assiri A.M., Peiris M., Gerber S.I, & Munster V.J. (2021). Surface-aerosol stability and pathogenicity of diverse MERS-CoV strains from 2012 – 2018. bioRxiv.

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Immunohistochemical Detection of MERS-CoV

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We fixed harvested tissues for >7 days in 10% neutral-buffered formalin, processed them by using a VIP-6 Tissue Tek Tissue Processor (Sakura Finetek, https://www.sakuraus.com), and embedded them in Ultraffin Paraffin Polymer (Cancer Diagnostics, https://www.cancerdiagnostics.com). We stained 5-μm sections with hematoxylin and eosin and detected coronavirus immunoreactivity by using MERS-CoV nucleocapsid protein rabbit antibody (diluted 1:4,000; Sino Biological Inc, https://www.sinobiological.com).
We processed tissues for immunohistochemical analysis by using the Discovery ULTRA Automated IHC/ISH Staining Instrument (and a Discovery ChromoMap DAB Kit (both from Ventana Medical Systems, https://diagnostics.roche.com). For morphometric analysis, we scanned slides by using the Aperio ScanScope AT2 (Aperio Technologies, Inc., https://www.aperio.com) and analyzed the entire section by using ImageScope Positive Pixel Count Algorithm version 9.1 (Aperio Technologies, Inc.). All tissue slides were evaluated by a board-certified veterinary anatomic pathologist.

van Doremalen N., Letko M., Fischer R.J., Bushmaker T., Schulz J., Yinda C.K., Seifert S.N., Kim N.J., Hemida M.G., Kayali G., Park W.B., Perera R.A., Tamin A., Thornburg N.J., Tong S., Queen K., van Kerkhove M.D., Choi Y.K., Oh M.D., Assiri A.M., Peiris M., Gerber S.I, & Munster V.J. (2021). Surface‒Aerosol Stability and Pathogenicity of Diverse Middle East Respiratory Syndrome Coronavirus Strains, 2012‒2018. Emerging Infectious Diseases, 27(12), 3052-3062.

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