Acetic acid, acetonitrile, methanol, gallic acid, rosmarinic acid, rutin, hydrochloric acid, sodium acetate, quercetin, Folin–Ciocâlteu reagent, protocatequic acid, gentisic acid, chlorogenic acid, catechin, caffeic acid, elagic acid, sinapic acid, ferulic acid, myricetin, tilirosid, quercetin, trans-cinnamic acid, luteolin were purchased from Sigma-Aldrich (Steinheim, Germany). Aluminum chloride, sodium acetate, sodium carbonate, ethanol was purchased from Merck, Darmstadt, Germany and DPPH (2,2-diphenyl-1-picrylhydrazyl) and BHT (butylated hydroxytoluene) were obtained from Alfa-Aesar (Germany). All microorganism strains were distributed by Oxoid®. All spectrophotometric data were acquired using a Jasco V-530 UV-Vis spectrophotometer (Jasco International Co., Ltd., Tokyo, Japan).
Elagic acid
Manufactured by Merck Group
Sourced in Germany, Poland
Elagic acid is a lab equipment product offered by Merck Group. It is a naturally occurring polyphenol compound. The core function of Elagic acid is to serve as a standard reference material for analytical and research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Gallic acid, by Merck Group (4 mentions)
Sinapic acid, by Merck Group (3 mentions)
Catechin, by Merck Group (2 mentions)
Caffeic acid, by Merck Group (2 mentions)
Chlorogenic acid, by Merck Group (2 mentions)
Rosmarinic acid, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Protocatechuic acid, by Merck Group (2 mentions)
Syringic acid, by Merck Group (2 mentions)
Sodium acetate, by Merck Group (1 mentions)
V 530 uv vis spectrophotometer, by Jasco (1 mentions)
Ferulic acid, by Merck Group (1 mentions)
Folin cioc lteu reagent, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Thermo Fisher Scientific (1 mentions)
Quercetin, by Merck Group (1 mentions)
Rutin, by Merck Group (1 mentions)
4 protocols using elagic acid
1
Phytochemical Extraction and Antioxidant Analysis
2
Quantitative Phenolic Compound Analysis
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MeOH, chloroform, and glacial acetic acid of analytical grade were purchased from Chempur. Water was purified by a Millipore water purification system. MeOH of HPLC grade was purchased from Merck. FeCl2 was obtained from Carlo Erba. Unless indicated otherwise, all chemicals were purchased from Sigma-Aldrich. Caffeic acid, chlorogenic acid, cinnamic acid, elagic acid, gallic acid, gentizic acid, isoferulic acid, neochlorogenic acid, o-coumaric acid, protocatechuic acid, rosmarinic acid, salicylic acid, sinapic acid, and syringic acid standards used for the HPLC analysis were purchased from Sigma Aldrich; p-coumaric acid, vanillic acid, ferulic acid, and p-hydroxybenzoic acid from Fluka; and cryptochlorogenic acid, isochlorogenic acid, and 4-O-feruloylquinic acid from ChromaDex.
3
Extraction and Analysis of Bioactive Compounds
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Acetonitrile, elagic acid, (+)-catechin, formic acid, gallic acid, methanol, methyl gallate, protocatechuic acid, and vanillin were purchased from Sigma-Aldrich Chemicals Co. (Poznan, Poland) and quercetin glucoside from Extrasynthese (Lyon, France). All other chemicals were reagent-grade products purchased from POCH (Gliwice, Poland).
4
Bioassay Protocol for Yeast Strains
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2.1 Reagents, microorganisms and cell lines 2,4-dinitrophenol, tannic acid, elagic acid, gallic acid, protecatechuic acid, vanillic acid, syringic acid and sinapic acid were purchased from Sigma-Aldrich and used as received. Ultra-pure water ( Mili-Q) was used to prepare all solutions. P. subcapitata and ephippia (dormant eggs) of D. magna, employed for the bioassays were supplied by ECOtest S.L. (Valencia, Spain) . Two recombinant strains of Saccharomyces cerevisiae were employed: BY4741 strain to detect estrogenic activity (ER strain) and YCM4 strain to monitor aryl hydrocarbon receptor agonist activity (AhR strain); they were kindly provided by Dr. Benjamin Piña from IDAEA-CSIC, Barcelona, Spain.
Medium constituents employed in bioassays were supplied by Difco (Basel, Switzerland). Prototrophic markers, standards (17-β-estradiol (E2) and βnaphthoflavone (β-NF)) and enzymatic substrate (4-methylumbelliferone β-Dgalactopyranoside and β-mercaptoethanol) were purchased form Sigma-Aldrich. Assay buffer (Triton X-100) and Y-PER (Yeast Protein Extraction Reagent) were supplied by Fisher Chemical and Thermo Scientific, respectively.
Medium constituents employed in bioassays were supplied by Difco (Basel, Switzerland). Prototrophic markers, standards (17-β-estradiol (E2) and βnaphthoflavone (β-NF)) and enzymatic substrate (4-methylumbelliferone β-Dgalactopyranoside and β-mercaptoethanol) were purchased form Sigma-Aldrich. Assay buffer (Triton X-100) and Y-PER (Yeast Protein Extraction Reagent) were supplied by Fisher Chemical and Thermo Scientific, respectively.
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