Log In Sign up
The largest database of trusted experimental protocols

Rodent decloaker solution

Manufactured by Biocare Medical
Visit Supplier

The Rodent Decloaker solution is a laboratory reagent used to retrieve antigens in formalin-fixed, paraffin-embedded rodent tissue samples. The solution is designed to effectively unmask target antigens, facilitating immunohistochemical analysis.

Automatically generated - may contain errors

Lab products found in correlation

Rodent block m, by Biocare Medical (2 mentions) Rabbit anti laminin, by Merck Group (1 mentions) Goat anti rabbit secondary antibody conjugated to alexa 488, by Thermo Fisher Scientific (1 mentions) Vs120 fluorescent slide scanner, by Olympus (1 mentions) Diaminobenzidine chromogen, by Agilent Technologies (1 mentions) Aperio cs2, by Leica (1 mentions) Betazoid dab chromogen, by Biocare Medical (1 mentions) Adamts12, by Abcam (1 mentions) Cat hematoxylin, by Biocare Medical (1 mentions) Axio imager fluorescence microscope, by Zeiss (1 mentions)

Spelling variants of this product

Decloaker (30 citations) Rodent Decloaker (13 citations)

4 protocols using rodent decloaker solution

1

Muscle Fiber Characterization via Histochemistry

Check if the same lab product or an alternative is used in the 5 most similar protocols
Formalin-fixed muscles were bisected and paraffin-embedded, and 10-μm cross sections were taken at the midbelly region. Sections were deparaffinized, rehydrated and subjected to Masson’s trichrome stain. In regard to immunohistochemitry, deparaffinized and rehydrated10-μm cross sections were subjected to antigen retrieval by heating in Rodent Decloaker solution (BioCare Medical, Pacheco, CA) at 80 °C for 30 min. Sections were blocked in 5% goat serum in PBS and incubated in rabbit anti-laminin (Sigma) diluted in 1:100 in blocking solution. Sections were then incubated in goat anti-rabbit secondary antibody conjugated to Alexa 488 (Life Technologies) diluted 1:200 in blocking solution. For detection of type II fibers sections were further incubated in anti-fast (type II) MHC conjugated to alkaline phosphatase (Sigma, St. Louis, MO) diluted 1:50 in PBS for 60 min at room temperature. Whole stained sections were scanned on an Olympus VS120 fluorescent slide scanner.
Cadena S.M., Zhang Y., Fang J., Brachat S., Kuss P., Giorgetti E., Stodieck L.S., Kneissel M, & Glass D.J. (2019). Skeletal muscle in MuRF1 null mice is not spared in low-gravity conditions, indicating atrophy proceeds by unique mechanisms in space. Scientific Reports, 9, 9397.
+ Open protocol
+ Expand
2

Quantitative Neutrophil Staining in Whole Footpads

Check if the same lab product or an alternative is used in the 5 most similar protocols
Whole footpads were harvested and fixed in 10% neutral buffered formalin for ≥24 hr. Tissue was decalcified using formic acid and paraffin embedded. Sections (5 μm thick) were de-paraffinized and rehydrated, and antigen retrieval was performed using Rodent Decloaker solution (Biocare Medical) at 80°C for 3 hr. Sections were stained with H&E, blocked with Rodent M Block (Biocare Medical), and labeled with anti-Ly-6G (clone 1A8-Ly6g, eBioscience).
Endogenous peroxidases were quenched with 3% H2O2, and antibody labeling was detected using horseradish peroxidase (HRP)-conjugated reagents and then visualized with diaminobenzidine chromogen (DakoCytomation). Quantitation of neutrophil staining was done using ImageJ software to integrate the total Ly-6G staining intensity in microscopic images (2× magnification) of whole-footpad sections.
Xu X., Pocock G.M., Sharma A., Peery S.L., Fites J.S., Felley L., Zarnowski R., Stewart D., Berthier E., Klein B.S., Sherer N.M, & Gumperz J.E. (2016). Human iNKT Cells Promote Protective Inflammation by Inducing Oscillating Purinergic Signaling in Monocyte-Derived DCs. Cell reports, 16(12), 3273-3285.
+ Open protocol
+ Expand
3

Immunohistostaining of Mouse Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse tissue processing and immunohistostaining were performed by the Pathology Core Laboratory at Tulane University Health Sciences Center (http://medicine.tulane.edu/departments/pathologylaboratorymedicine/research/histology-laboratory) as described previously.19 (link), 20 (link) Heat-induced epitope retrieval was performed on tissue sections by using Rodent Decloaker solution (BioCare Medical, Concord, CA; RD913) and cooked in an oster steamer for 40 minutes. Sections were blocked by using Rodent Block M (BioCare Medical; RBM961), followed by incubation with the following antibodies: ADAMTS12 (Abcam; cat 203012, lot GR2390174), ITGA2 (Abcam; cat 133557, lot GR19622312), ST8SIA5 (Abcam; cat 184777, lot GR2231323), and Ki67 (1:100, 45 minutes; BioCare Medical; CRM325). After washing, tissue sections were incubated with Rabbit-on-Rodent HRP-Polymer secondary (BioCare Medical; RMR622); sections were then washed and treated with Betazoid DAB chromogen (Biocare Medical; BDB2004), followed by counterstaining with Cat hematoxylin (Biocare Medical; CATHEM). Slides were dried in the oven, placed in xylene, and coverslipped (Acrymount; StatLab, McKinney, TX; SL804). Images were obtained by using the slide scanner Aperio CS2 (Leica, Wetzlar, Germany) and Image Scope software.
Penrose H.M., Cable C., Heller S., Ungerleider N., Nakhoul H., Baddoo M., Hartono A.B., Lee S.B., Burow M.E., Flemington E.F., Crawford S.E, & Savkovic S.D. (2018). Loss of Forkhead Box O3 Facilitates Inflammatory Colon Cancer: Transcriptome Profiling of the Immune Landscape and Novel Targets. Cellular and Molecular Gastroenterology and Hepatology, 7(2), 391-408.
+ Open protocol
+ Expand
4

Quantifying Kidney Tissue Injury and Angiogenesis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Kidney sections in 7-μm thickness were deparaffmized [25 (link)], heated in Rodent Decloaker solution (Biocare Medical) for 30 min to retrieve antigens, and blocked with 1% BSA and 0.1% Triton-100 in PBS for 1 h. Tissue sections were incubated with FITC- or Alexa Fluor 594-labeled anti-CD31 mAb (Biolegend) at 4oC overnight. Nuclei were stained with DAPI. Tissue sections were analyzed under an AxioImager fluorescence microscope (Carl Zeiss). Furthermore, tissue sections were stained with hematoxylin and eosin (H&E) for structural examination using a light microscope.
Tubular injury was evaluated in each viewing field. Tubular injury was defined as tubular dilatation, tubular atrophy and vacuolization. Briefly, only cortical tubules were quantified with the following scoring system: 0 = no tubular injury; 1 = <10% of tubules injured; 2 = 10–25% of tubules injured; 3 = 26–50% of tubules injured; 4 = 51–75% of tubules injured; 5 = >75% of tubules injured [26 (link)]. The size of individual glomeruli was calculated as the average of the largest and smallest glomerular diameters within viewing fields [27 (link)]. Vessel density was quantified using Photoshop [13 (link)].
Tang F., LeBlanc M.E., Wang W., Liang D., Chen P., Chou T.H., Tian H, & Li W. (2019). Anti-Secretogranin III Therapy of Oxygen-Induced Retinopathy with Optimal Safety. Angiogenesis, 22(3), 369-382.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!