Barley β glucan

Barley β-glucan (80% purity), laminarin, carboxymethyl cellulose (CMC), xylan, curdlan, avicel, and chitosan were purchased from Shanghai McLean Biochemical Technology Co., Ltd. Lichenin was purchased from Megazyme (Ireland).
Bacillus subtilis B110 strain was acquired from the Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources of Jiangxi Province and maintained in a lysogeny broth. Escherichia coli DH5α (Invitrogen Co., China) was used as the host strain for gene manipulation. E. coli BL21 (DE3) (Novagen Co., China) was used for recombinant expression. Plasmid pET-29a (TakaRa Biotechnology, China) served as the expression vector.

Glucose and other cello-oligosaccharide (G₂-G₅), carboxymethyl-cellulose (CMC), barley β-glucan, laminarin, xylan (from corncob), avicel, chitosan, and filter paper were obtained from McLean Biochemical Technology Co., Ltd. (Shanghai, China), whereas lichenin was purchased from Megazyme (Bray, Ireland). Other chemicals used in this study were provided by Sigma-Aldrich (Shanghai, China).
Myxococcus sp. B6-1 was isolated from soil and cultured in a lysogeny broth (LB). DH5 and BL21 (DE3) strains of E. coli from our laboratory were respectively utilized for gene cloning and expression. Plasmid pET-29a (Takara, Beijing, China) was used for expression.