Inverted research microscope

Manufactured by Nikon

The Inverted Research Microscope is a laboratory instrument designed for detailed observation and analysis of samples. It features a reversed optical path, allowing the sample to be viewed from below. This configuration enables the use of specialized sample holders and live cell imaging.

Automatically generated - may contain errors

Lab products found in correlation

Cell meter 2 nbdg glucose uptake assay kit, by AAT Bioquest (1 mentions) Microplate reader, by Agilent Technologies (1 mentions) Oro staining kit, by Solarbio (1 mentions) Bca protein assay kit, by Beyotime (1 mentions)

4 protocols using inverted research microscope

Adipose Tissue Glucose Uptake Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Adipose tissues' glucose uptake function was measured using a Cell Meter™ 2-NBDG Glucose Uptake Assay Kit (36702, AAT Bioquest). Fresh adipose tissue was incubated with 0.5 mM 2-NBDG for 1 hour in a 37°C, 5% CO₂ incubator. The fluorescence signal of 2-NBDG (Ex. @ 488 nm/Em. @ 530 nm) was monitored using the Nikon Inverted Research Microscope with a 20× 0.75 NA objective. We captured at least three Z-stack images of each adipose tissue (~20 μm depth of field, 2 μm step size), and used the Image J software to perform maximum intensity projection of Z-stack images to quantify the fluorescence intensity of each image.

Chen L., Qin G., Liu Y., Li M., Li Y., Guo L.Z., Du L., Zheng W., Wu P.C., Chuang Y.H., Wang X., Wang T.D., Ho J.A, & Liu T.M. (2023). Label-free optical metabolic imaging of adipose tissues for prediabetes diagnosis. Theranostics, 13(11), 3550-3567.

+ Open protocol

+ Expand

Transwell Assay for Cell Migration

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell migration and invasion were measured by Transwell assay using the 24-well Transwell chamber containing 8 μm polycarbonate membranes. By cell counting, approximately 1 × 10⁵ cells were seeded in the upper chamber containing 200 μL of serum-free medium. Then, the lower chamber was inserted into the 24 wells containing 500 μL of 10% DMEM. After incubation at 37°C for 24 hours in the cell incubator, cells were scraped off of the membrane surface. Cells were fixed with methanol and stained with 0.5% crystal violet. Each well was photographed with a Nikon inverted research microscope and counted in not less than three random 100x microscope fields.

Ji R., Ji Y., Ma L., Ge S., Chen J., Wu S., Huang T., Sheng Y., Wang L., Yi N, & Liu Z. (2021). Keratin 17 upregulation promotes cell metastasis and angiogenesis in colon adenocarcinoma. Bioengineered, 12(2), 12598-12611.

+ Open protocol

+ Expand

Wound Healing Assay for MCF-7 Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The MCF-7 cell lines were first grown in DMEM supplemented with 10% FBS to test migration assay, then seeded in 6-well plates.^{19 (link)} Next, the width of each well is scratched by sterile pipette tip (1 mm). The wells are washed by the culture medium and the picture of wounds is taken at 0 hour (NIKON, Inverted research microscope). Thereafter, medium was replaced with different concentrations of PLGA-PTX-VitD₃ co-delivery NPs, PTX and PLGA-PTX (IC₅₀ concentration) and the cells are allowed to migrate for 24 hours. After 24 hours, the picture of wound is taken from the same regions. The rate of migration (R_M) is analyzed by measuring the distance between the scratch edges with digitizer software. The R_M is calculated by following equation: R_M=(W_i-W_f)/t.^{20 (link)}

Khodaverdi S., Jafari A., Movahedzadeh F., Madani F., Yousefi Avarvand A, & Falahatkar S. (2019). Evaluating Inhibitory Effects of Paclitaxel and Vitamin D3 Loaded Poly Lactic Glycolic Acid Co-Delivery Nanoparticles on the Breast Cancer Cell Line. Advanced Pharmaceutical Bulletin, 10(1), 30-38.

+ Open protocol

+ Expand

Quantitative Lipid Droplet Analysis in Adipocytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

An ORO staining kit (Solarbio) was used to stain lipid droplets in mature 3T3-L1- and C2C12-derived adipocytes. In brief, cells were washed twice with PBS and fixed with ORO fixative at room temperature for 20 min. Then, cells were rinsed with 60% isopropyl alcohol and stained with ORO dye for 20 min. After the ORO dye was washed away with distilled water, ORO-stained cells were visualized with an inverted research microscope (Nikon). For quantification, intracellular ORO was extracted with 100% isopropanol and quantified by measuring the optical absorbance at 520 nm using a microplate reader (BioTek Instruments). Then, the total protein was detected by a BCA protein assay kit (Beyotime) at 562 nm with a microplate reader (BioTek Instruments). The relative ORO value was calculated as OD_ORO-520 values to total protein level.

Yu W., Chen C.Z., Peng Y., Li Z., Gao Y., Liang S., Yuan B., Kim N.H., Jiang H, & Zhang J.B. (2021). KRAS Affects Adipogenic Differentiation by Regulating Autophagy and MAPK Activation in 3T3-L1 and C2C12 Cells. International Journal of Molecular Sciences, 22(24), 13630.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!