Validated siRNA duplex constructs targeted against human PDGFB were purchased from Sigma Aldrich (SASI_Hs01_00121961, SASI_Hs01_00121962, (St. Louis, MO, USA). Mission siRNA Transfection reagent (Sigma Aldrich) was used to reverse transfect 10 nM of siRNA to MSC, in a 24-well format following manufacturer’s protocols. A universal negative siRNA non-targeting control (Sigma Aldrich) was utilized to rule out effects from transfection protocols. RNA was extracted from MSC treated with siRNA (siPDGFB MSC) to assess the amount of knockdown via qPCR, outlined below.
Sasi hs01 00121961
Manufactured by Merck Group
Sourced in United States
SASI_Hs01_00121961 is a laboratory equipment product from Merck Group. It is designed for performing specific laboratory tasks. The core function of this product is to enable efficient and accurate data collection and analysis within the intended laboratory setting. The detailed specifications and intended use case for this product are not available in this response.
Automatically generated - may contain errors
2 protocols using sasi hs01 00121961
1
PDGFB Knockdown in Mesenchymal Stem Cells
2
PDGFB Silencing via siRNA
Check if the same lab product or an alternative is used in the 5 most similar protocols
PDGFB expression was knocked down with predesigned siRNA SASI_Hs01_00121961 and SASI_Hs01_00121962 (Sigma Aldrich), which were co‐transfected. As a negative control, we used a scrambled siRNA (Sigma Aldrich): 5’‐CUUUGGGUGAUCUACGUUA[dT]‐3’. siRNAs were transfected using the transfection reagent jetPRIME (Polyplus, Illkirch, France) following the manufacturer's instructions.
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PDGFB expression was knocked down with predesigned siRNA SASI_Hs01_00121961 and SASI_Hs01_00121962 (Sigma Aldrich), which were co‐transfected. As a negative control, we used a scrambled siRNA (Sigma Aldrich): 5’‐CUUUGGGUGAUCUACGUUA[dT]‐3’. siRNAs were transfected using the transfection reagent jetPRIME (Polyplus, Illkirch, France) following the manufacturer's instructions.
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