Anti muc1 c

Manufactured by Cell Signaling Technology

Sourced in United States

Anti-MUC1-C is a laboratory reagent used in research applications. It is an antibody that specifically binds to the MUC1-C protein. MUC1-C is a transmembrane glycoprotein involved in cell signaling processes. The function of this antibody is to detect and study the expression and localization of MUC1-C in experimental samples.

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Lab products found in correlation

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Spelling variants of this product

MUC1-C (4 citations) Anti-MUC1 (4 citations)

6 protocols using anti muc1 c

Immunoblotting analysis of cellular signaling

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Whole cell lysates were prepared in RIPA buffer containing protease inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA). Immunoblotting was performed with anti-MUC1-C (#16564, 1:1000 dilution; Cell Signaling Technology (CST), Danvers, MA, USA), anti-RIG-I (#3743, 1:1000 dilution; CST), anti-MDA5 (#5321, 1:1000 dilution; CST), anti-cGAS (#15102, 1:1000 dilution; CST), anti-STING (#13647, 1:1000 dilution; CST), anti-STAT1 (#9172, 1:1000 dilution; CST), anti-STAT2 (#72604, 1:1000 dilution; CST), anti-IRF1 (#8478, 1:1000 dilution; CST), anti-IRF9 (#76684, 1:1000 dilution; CST), anti-ISG15 (sc-166755, 1:250 dilution; Santa Cruz, Santa Cruz, CA, USA), anti-PARP1 (#9532, 1:1000 dilution; CST), anti-γH2AX (#9718, 1:1000 dilution; CST) and anti-β-actin (A5441; 1:50000 dilution; Sigma, St. Louis, MO, USA).

Yamashita N., Fushimi A., Morimoto Y., Bhattacharya A., Hagiwara M., Yamamoto M., Hata T., Shapiro G.I., Long M.D., Liu S, & Kufe D. (2022). Targeting MUC1-C Suppresses Chronic Activation of Cytosolic Nucleotide Receptors and STING in Triple-Negative Breast Cancer. Cancers, 14(11), 2580.

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Immunoblotting Analysis of Cell Signaling

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Whole-cell lysates were prepared in RIPA buffer containing protease inhibitor cocktail (ThermoFisher Scientific). Immunoblotting was performed with anti-MUC1-C (#16564S, 1:1000 dilution; Cell Signaling Technology (CST), Danvers, MA, USA), anti-NF-κB p65 (#8242S, 1:1000 dilution; CST), anti-phospho-NF-κB p65 (#3037s, 1:1000 dilution; CST), anti-GAPDH (5174, 1:1000 dilution; CST), anti-RBM15B (22249-1-AP, 1:1000 dilution; Proteintech, Rosemont, IL, USA), anti-RBM15 (10587-1-AP, 1:5000 dilution; Proteintech), anti-YTHDF2 (24744-1-AP, 1:5000 dilution; Proteintech), anti-CNOT1 (14276-1-AP, 1:1000 dilution; Proteintech), anti-WTAP (#56501, 1:1000 dilution; CST), anti-METTL3 (#96391, 1:1000 dilution; CST), anti-METTL14 (#51104, 1:1000 dilution; CST), anti-IGF2BP1 (22803-1-AP, 1:5000 dilution; Proteintech), anti-TDP-43 (#32654, 1:1000 dilution; CST), anti-NOTCH1 (#3608S, 1:1000 dilution; CST), anti-BMI1 (#6964P, 1:1000 dilution; CST), anti-CD44 (#5640S, 1:1000 dilution; CST), anti-β-actin (A5441; 1:50000 dilution; Sigma, St. Louis, MO, USA) and anti-tubulin (#2144S, 1:1000 dilution; CST). Signals shown in immunoblots and in a separate biologic replicate were each scanned in triplicate. The results (mean ± SD of six determinations) are expressed as relative signal intensity compared to that obtained for GAPDH.

Wang K., Bhattacharya A., Haratake N., Daimon T., Nakashoji A., Ozawa H., Peng B., Li W, & Kufe D. (2024). XIST and MUC1-C form an auto-regulatory pathway in driving cancer progression. Cell Death & Disease, 15(5), 330.

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Immunoblot Analysis of Stem Cell Markers

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Total lysates prepared from subconfluent cells were subjected to immunoblot analysis. Immunoblotting was performed with anti-MUC1-C (#16564, 1:1000 dilution; Cell Signaling Technology (CST), Danvers, MA), anti-MYC (#5605, 1:1000 dilution; CST), anti-OCT4 (#2750, 1:1000 dilution; CST), anti-SOX2 (#3579, 1:1000 dilution; CST), anti-KLF4 (#12173, 1:1000 dilution; CST), anti-BRN2 (#12137, 1:1000 dilution; CST), anti-ASCL1 (#GTX129189, 1:2000 dilution; GeneTex, Irvine, CA), anti-SYP (#MA5-16402, 1:200 dilution; Thermo Fisher Scientific, Waltham, MA), anti-AURKA (#ab1287, 1:2000 dilution; Abcam), anti-NOTCH1 (#3608, 1:1000 dilution; CST), anti-NOTCH2 (#5732, 1:1000 dilution; CST) and anti-GAPDH (#5174, 1:2000 dilution; CST).

Luan Z., Morimoto Y., Fushimi A., Yamashita N., Suo W., Bhattacharya A., Hagiwara M., Jin C, & Kufe D. (2021). MUC1-C dictates neuroendocrine lineage specification in pancreatic ductal adenocarcinomas. Carcinogenesis, 43(1), 67-76.

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Immunoprecipitation and Immunoblotting Analysis

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Cell lysates were prepared as described (22 ). Soluble proteins (1 mg) were immunoprecipitated with anti-EGFR (Cell Signaling Technology) or a control IgG. The precipitates and lysates not subjected to immunoprecipitation were analyzed by immunoblotting with anti-MUC1-C (31 (link)), anti-p-EGFR(Tyr-1148) (Cell Signaling Technology), anti-EGFR (Abcam), anti-p-AKT(Ser-473), anti-AKT, anti-p-MEK(Ser-217/221), anti-MEK, anti-p-ERK(Thr-202/Tyr-204), anti-ERK (Cell Signaling Technologies) or anti-β-actin (Sigma) as described (22 ). Immune complexes were detected with horseradish peroxidase-conjugated secondary antibodies and enhanced chemiluminescence (GE Healthcare).

Kharbanda A., Rajabi H., Jin C., Tchaicha J., Kikuchi E., Wong K.K, & Kufe D. (2014). TARGETING THE ONCOGENIC MUC1-C PROTEIN INHIBITS MUTANT EGFR-MEDIATED SIGNALING AND SURVIVAL IN NON-SMALL CELL LUNG CANCER CELLS. Clinical cancer research : an official journal of the American Association for Cancer Research, 20(21), 5423-5434.

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Immunoblotting Analysis of Immune Signaling

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Whole-cell lysates were prepared in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitor cocktail (Thermo Fisher Scientific). Immunoblotting was performed with anti-MUC1-C (#16564, 1:1000 dilution; Cell Signaling Technology (CST), Danvers, Massachusetts, USA), anti-STAT1 (9172S, 1:1000 dilution; CST), anti-STAT1 phosphorylation (pSTAT1) (Y701) (#7167S, 1:1000 dilution; CST), anti-JAK1 (#3332S, 1:1000 dilution; CST), anti-IFN-γ (#8455S, 1:1000 dilution; CST), anti-IRF1 (#8478S, 1:1000 dilution; CST), anti-IDO1 (#86 630S, 1:1000 dilution; CST), anti-COX2 (#12 282S, 1:1000 dilution; CST), anti-prostaglandin E synthase (PTGES)(#ab62050, 1:1000 dilution; Abcam, Cambridge, Massachusetts, USA), anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (#5174S, 1:1000 dilution; CST) and anti-β-actin (A5441, 1:100 000 dilution; Sigma).

Yamashita N., Long M., Fushimi A., Yamamoto M., Hata T., Hagiwara M., Bhattacharya A., Hu Q., Wong K.K., Liu S, & Kufe D. (2021). MUC1-C integrates activation of the IFN-γ pathway with suppression of the tumor immune microenvironment in triple-negative breast cancer. Journal for Immunotherapy of Cancer, 9(1), e002115.

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Immunoblotting Analysis of Cell Signaling

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Whole cell lysates were prepared in RIPA buffer containing protease inhibitor cocktail (Thermo Fisher Scientific, Waltham, MA, USA). Immunoblotting was performed with anti-MUC1-C (16564, 1:1000 dilution; Cell Signaling Technology (CST), Danvers, MA, USA), anti-STAT1 (9172S, 1:1000 dilution; CST), anti-IRF1 (#8478S, 1:1000 dilution; CST), anti-IFNGR1 (34808, 1:1000; CST), anti-GAPDH (#5174S, 1:1000 dilution; CST) and anti-β-actin (A5441; 1:100000 dilution; Sigma, St. Louis, MO, USA), anti-FBXW7 (ab109617, 1:1000; abcam, Cambridge, MA, USA), anti-MTA1 (5647, 1:1000; CST), anti-MBD3 (14540, 1:1000; CST), anti-IDO1 (86630, 1:1000; CST), anti-WARS (GTX110223, 40037, 1:1000; Gene Tex), anti-PTGES (ab180589, 1:1000; abcam), anti-PBRM1(A301-591A, 1:10000; Bethyl Laboratories, Montgomery, TX, USA), anti-ARID1A (12354, 1:500; CST), anti-ISG15 (sc166755, 1:2000; Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-SERPINB9 (PA5-51038, 1:2000; Invitrogen, Waltham, MA, USA).

Hagiwara M., Fushimi A., Bhattacharya A., Yamashita N., Morimoto Y., Oya M., Withers H.G., Hu Q., Liu T., Liu S., Wong K.K., Long M.D, & Kufe D. (2022). MUC1-C integrates type II interferon and chromatin remodeling pathways in immunosuppression of prostate cancer. Oncoimmunology, 11(1), 2029298.

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