Alphascreen cAMP Assay kit (6760635M, PerkinElmer) was used to measure cAMP in the samples. Treated cell pellets were re-suspended in stimulation buffer that was prepared fresh from 1x HBSS, 5 mM HEPES, 0.5 mM IBMX and 0.1% BSA. cAMP assays were performed following manufacturer’s instructions and forskolin (10 μM final concentration) was added into each well to stimulate cAMP production. A cAMP standard curve was created from fluorescent signals of serial dilutions of cAMP standard solution. A Spectramax i3X microplate reader with alphascreen module and SolfMax Pro software (Molecular Devices) was used for signal detection and to measure cAMP accumulation.
Solfmax pro software
Manufactured by Molecular Devices
SoftMax Pro software is a data acquisition and analysis software designed for use with Molecular Devices' microplate readers. It provides a user-friendly interface for controlling the instrument, collecting data, and performing various analytical functions.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using solfmax pro software
1
Measuring cAMP in Treated Cells
2
Measuring cAMP in Treated Cells
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Alphascreen cAMP Assay kit (6760635M, PerkinElmer) was used to measure cAMP in the samples. Treated cell pellets were re-suspended in stimulation buffer that was prepared fresh from 1x HBSS, 5 mM HEPES, 0.5 mM IBMX and 0.1% BSA. cAMP assays were performed following manufacturer’s instructions and forskolin (10 μM final concentration) was added into each well to stimulate cAMP production. A cAMP standard curve was created from fluorescent signals of serial dilutions of cAMP standard solution. A Spectramax i3X microplate reader with alphascreen module and SolfMax Pro software (Molecular Devices) was used for signal detection and to measure cAMP accumulation.
3
Measuring cAMP with Alphascreen Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Alphascreen cAMP Assay kit (6760635M, PerkinElmer) was used to measure cAMP in the samples. Treated cell pellets were re-suspended in stimulation buffer that was prepared fresh from 1x HBSS, 5 mM HEPES, 0.5 mM IBMX and 0.1% BSA. cAMP assays were performed following manufacturer's instructions and forskolin (10 μM final concentration) was added into each well to stimulate cAMP production. A cAMP standard curve was created from fluorescent signals of serial dilutions of cAMP standard solution. A Spectramax i3X microplate reader with alphascreen module and SolfMax Pro software (Molecular Devices) was used for signal detection and to measure cAMP accumulation.
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