Cocl2

The constructed plasmid pET21a-CaCDC42 containing the cdc42 gene of Candida albicans, which served as the template DNA, was recombined and prepared in the College of Life Science, Beijing Normal University, China. SYBR® Premix Ex Taq™ II containing Taq DNA polymerase catalyzing the elongation step of DNA replication, dNTPs, ROX Reference Dye II, DNA loading buffer, and SYBR Green II was purchased from Takara Bio Inc. (Dalian, China). The synthetic primers targeting cdc42 were provided by Sangon Biotech Co. Ltd. (Beijing, China) as follows: 5′-GTGATGGTGCCGTTGGTA-3′ (forward) and 5′-CCCTGTTCCTGGGTGATT-3′ (reverse), which produced a piece of DNA containing 397 bp. Thirteen heavy metal salts, ZnCl₂, Pb(NO₃)₂, CdCl₂·2.5H₂O, Ni(NO₃)₂·6H₂O, HgCl₂, CrCl₃, MnCl₂, CuSO₄, CoCl₂, Ce(NO₃)₃, Sr(NO₃)₂, Bi(NO₃)₃ and K₃Fe(CN)₆ were obtained from Beijing Chemical Reagent Company (Beijing, China). Ethylene diamine tetraacetic acid (EDTA), Ethidium bromide (EB), and agarose was from Sigma-Aldrich (St. Louis, USA). The DNA replication reactions in vitro were fulfilled and monitored using the Real-time PCR System ABI7500 (Applied Biosystems™, USA).