C57bl 6 c57

Islets were isolated from 10 to 12 weeks old male mice of the inbred C57BL/6 (C57; Charles River) and outbred Crl:CD1 (ICR) mice (Charles River) strains by collagenase digestion of the exocrine pancreas [16 (link)]. All animal procedures were carried out in accordance with the UK Home Office Animals (Scientific Procedures) Act 1986. Human islets were isolated from heart-beating non-diabetic donors as previously described [17 (link)], with appropriate ethical approval for research use. Isolated mouse and human islets were maintained in culture overnight (mouse: RPMI 1640; human: CMRL) at 37 °C, 5% CO₂ before experimental use.

Islets were isolated from 10–12 week old male mice of the inbred C57BL/6 (C57; Charles River) and outbred ICR (Harlan) strains by collagenase digestion of the exocrine pancreas29 (link). All animal procedures were approved by the King’s College London Ethics Committee and carried out in accordance with the UK Home Office Animals (Scientific Procedures) Act 1986.
Human islets were isolated from non-diabetic organ donors at the King’s College London Human Islet Isolation Unit30 (link) with appropriate ethical approval and donor consent for research (Protocol number 01–082, Human Islet Isolation and Research). An assent form was completed by a relative of the pancreas donor for all islets used for research, and all experiments carried out on human islets were performed in accordance with the relevant guidelines and regulations as set out by the King’s College London Ethics Committee.
Isolated mouse and human islets were maintained in culture overnight (mouse: RPMI 1640; human: CMRL) at 37 °C, 5% CO₂ before extraction of RNA.