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Mouse rat leptin quantikine elisa

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The Mouse/Rat Leptin Quantikine ELISA is an enzyme-linked immunosorbent assay designed for the quantitative determination of mouse and rat leptin levels in cell culture supernatants, serum, and plasma.

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Lab products found in correlation

Infinite m200 i control, by Tecan (2 mentions) Nesfatin 1 1 82aa rat eia kit, by Phoenix Pharmaceuticals (2 mentions) Mouse adiponectin elisa kit, by Merck Group (2 mentions) Centrifuge 5424, by Eppendorf (1 mentions) Triglyceride colorimetric assay kit, by Cayman Chemical (1 mentions) Contour xt, by Bayer (1 mentions) Ultra sensitive mouse insulin elisa, by Crystal Chem (1 mentions)

Close spelling variants of this product

Quantikine® ELISA Mouse/Rat Leptin Immunoassay Mouse/Rat Leptin Quantikine ELISA Kit (SMOB00) Mouse/Rat Leptin Quantikine ELISA Kit Quantikine Mouse/Rat Leptin Mouse leptin ELISA Quantikine ELISA Leptin Quantikines ELISAs

3 protocols using mouse rat leptin quantikine elisa

1

Plasma Biomarkers in Naïve Blood Samples

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Directly after blood collection, blood glucose concentration in naïve blood samples was assessed with a glucose meter (Contour®XT, Bayer, Leverkusen, Germany). For plasma preparation, blood samples were centrifuged at 1200 rpm and 6 °C for 10 min (Centrifuge 5424, Eppendorf, Leipzig, Germany). Afterwards, the supernatant was collected and stored at −80 °C. From stored plasma samples, measurement of plasma triglycerides was performed using Triglyceride Colorimetric Assay Kit (Nr. 10010303, Cayman Chemical Company, Hamburg, Germany). Additionally, the stored plasma samples were used to perform insulin (Ultra Sensitive Mouse Insulin ELISA, Crystal Chem, Zaandam, The Netherlands) and leptin ELISAs (Mouse/Rat Leptin Quantikine ELISA, R&D Systems, Abingdon, UK) according to the manufacturer’s instructions.
Müller L., Power Guerra N., Schildt A., Lindner T., Stenzel J., Behrangi N., Bergner C., Alberts T., Bühler D., Kurth J., Krause B.J., Janowitz D., Teipel S., Vollmar B, & Kuhla A. (2023). [18F]GE-180-PET and Post Mortem Marker Characteristics of Long-Term High-Fat-Diet-Induced Chronic Neuroinflammation in Mice. Biomolecules, 13(5), 769.
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2

Quantifying Serum Biomarkers in Mice

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The sera from mice were collected and stored at −80°C and used to quantify the concentration of Nucleobindin-2(Nucb2) in serum using the CUSABIO Mouse Nucb2 Elisa kit (CSB-EL016146MO). ELISAs were read on the Tecan Infinite M200 i-control. Nesfatin-1 was measured using nesfatin-1 (1-82aa) (rat) EIA kit (EK-003-22; Phoenix Pharmaceuticals; Burlingame, CA, USA) with a detectable range of 0.1-1000ng/ml. Adiponectin was measured using Mouse Adiponectin ELISA kit (EMD Millipore; St. Charles, Missouri, USA). Leptin was measured using Mouse/Rat Leptin Quantikine ELISA (R&D Systems; Minneapolis, MN, USA).
Ravussin A., Youm Y.H., Sander J., Ryu S., Nguyen K., Varela L., Suhlman G.I., Sidorov S., Horvath T.L., Schultze J.L, & Dixit V.D. (2018). Loss of Nucleobindin-2 Causes Insulin Resistance in Obesity without Impacting Satiety or Adiposity. Cell reports, 24(5), 1085-1092.e6.
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3

Quantifying Serum Biomarkers in Mice

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The sera from mice were collected and stored at −80°C and used to quantify the concentration of Nucleobindin-2(Nucb2) in serum using the CUSABIO Mouse Nucb2 Elisa kit (CSB-EL016146MO). ELISAs were read on the Tecan Infinite M200 i-control. Nesfatin-1 was measured using nesfatin-1 (1-82aa) (rat) EIA kit (EK-003-22; Phoenix Pharmaceuticals; Burlingame, CA, USA) with a detectable range of 0.1-1000ng/ml. Adiponectin was measured using Mouse Adiponectin ELISA kit (EMD Millipore; St. Charles, Missouri, USA). Leptin was measured using Mouse/Rat Leptin Quantikine ELISA (R&D Systems; Minneapolis, MN, USA).
Ravussin A., Youm Y.H., Sander J., Ryu S., Nguyen K., Varela L., Suhlman G.I., Sidorov S., Horvath T.L., Schultze J.L, & Dixit V.D. (2018). Loss of Nucleobindin-2 Causes Insulin Resistance in Obesity without Impacting Satiety or Adiposity. Cell reports, 24(5), 1085-1092.e6.
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