The HPLC grade: methanol (CAS 67-56-1) and acetonitrile (CAS 75-05-8) (from Tedia, Fairfield, CT, USA); and analytical reagent grade: acetonitrile (for robustness test); ammonium acetate (CAS 631-61-8); potassium dihydrogen phosphate (CAS 7778-77-0); phosphoric acid 85% (CAS 7664-38-2) and acetic acid (CAS 64-19-7) (from Merck, Darmstadt, Germany). Ultrapure water (type 1 water—18.2 MΩ·cm)/conductivity 0.055 μS/cm at 25 °C) was obtained by a Milli-Q water system by Merck (Darmstadt, Germany).
Phosphoric acid 85
Manufactured by Merck Group
Sourced in Germany
Phosphoric acid 85% is a commonly used laboratory reagent. It is a clear, colorless, viscous liquid with a strong acidic odor. The primary function of this product is to serve as a source of phosphoric acid, which is widely employed in various chemical processes and analyses.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Merck Group (4 mentions)
Methanol, by Merck Group (3 mentions)
Potassium dihydrogen phosphate, by Merck Group (2 mentions)
Ammonium acetate, by Merck Group (2 mentions)
Benzoic acid, by Merck Group (2 mentions)
Dichloromethane, by Merck Group (2 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Sodium chloride (nacl), by Merck Group (2 mentions)
Glacial acetic acid, by Merck Group (2 mentions)
Ethanol, by Merck Group (2 mentions)
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Milli q water system, by Merck Group (1 mentions)
Acetic acid, by Merck Group (1 mentions)
Acetonitrile, by Tedia (1 mentions)
Peptone water, by Thermo Fisher Scientific (1 mentions)
Acetic acid, by Honeywell (1 mentions)
Malic acid, by Merck Group (1 mentions)
Succinic acid, by Merck Group (1 mentions)
0.2 μm membrane, by Merck Group (1 mentions)
Propionic acid, by Merck Group (1 mentions)
12 protocols using phosphoric acid 85
1
HPLC Reagent Preparation Protocol
2
Quantitative Analysis of Organic Acids
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The reagents used were all of analytical grade (purity > 98%): DL-lactic acid and butyric acid purchased from Fluka (Buchs, Switzerland), acetic acid from Riedel-de-Haën (Seelze, Germany), L-(+)-tartaric acid, formic, citric acid, benzoic acid, succinic acid, malic acid, propionic acid, DL-p-hydroxyphenyllactic acid (HO-PLA), sodium hydroxide, sodium chloride, glycerol, and phenyllactic acid (PLA) bought from Sigma-Aldrich (Germany). Phosphoric acid 85% and oxalic acid were supplied by Merck (Hamburg, Germany), cetyltrimethylammonium bromide (CTAB) from LOBA Chemie (Fischamend, Austria), HPLC-grade water and 0.1 and 1 N sodium hydroxide solutions were purchased from Agilent Technologies (USA). All solvents (Merck, Darmstadt, Germany) and solutions were filtered through 0.2-μm membranes (Millipore, Bedford, MA, USA). Peptone water and MRS broth media were purchased from Oxoid Ltd. (Hampshire, UK).
3
Chlorpyrifos extraction and analysis
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Dichloromethane, phosphoric acid (85 %), and acetonitrile of HPLC grade were purchased from Merck (Darmstadt, Germany). Chlorpyrifos was purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA 95060, USA).
4
Ibuprofen, Menthol, and Camphor Formulation
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Ibuprofen, menthol (M), and camphor (C) were purchased from P.C. Drug Center Co. Ltd. (Bangkok, Thailand); Tween 80 from VWR International Ltd. (Bournemouth, UK); phosphoric acid 85% from Merck KGaA (Darmstadt, Germany); methanol, acetonitrile, and chloroform from SK chemicals Co. Ltd. (Ulsan, Korea); sodium hydroxide and potassium dihydrogen phosphate from QReC (Auckland, New Zealand); glucose and sodium benzoate from Ajax Finechem Pty. Ltd. (Auckland, New Zealand); xanthan gum (Xantural® 75) from CP Kelco Shandong Biological Company Ltd. (Shanghai, People’s Republic of China); and ibuprofen suspension (Nurofen® 100 mg/5 mL) from Reckitt Benckiser (Thailand) Ltd. (Bangkok, Thailand).
5
HPLC Analysis of Phospholipid-Cholesterol Product
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Phospholipon® 90 H and cholesterol products were obtained from Shanghai Soyoung Biotech. Inc. (Shanghai, China). The VPA reference standard was provided by Octagon Chemical Limited (Yantai, China). Sartorius membrane D0405-100FT dialysis tubing was provided by Sigma-Aldrich (St. Louis, USA). Disodium hydrogen phosphate, potassium dihydrogen phosphate, sodium chloride, potassium chloride, acetonitrile, methanol, glacial acetic acid, and dichloromethane were obtained from Merck (Darmstadt, Germany). Polycarbonate membranes (0.45 μm) and 0.22-μm cellulose acetate membranes were obtained from Whatman. All other reagents were of analytical grade or HPLC grade from Merck (Darmstadt, Germany). Benzoic acid as an internal standard was purchased from Sigma-Aldrich. methanol (high-performance liquid chromatography [HPLC] grade), acetonitrile (HPLC grade), phosphoric acid 85% (analytical grade), sodium dihydrogen phosphate, acetic acid glacial, ammonium acetate, formic acid, ammonium formate, hydrochloride acid, triethylamine, n-hexane, and ethyl acetate were purchased from Merck. Double distilled water was used (Ikapharmindo).
6
Analytical Method for MXC and PXC
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The substances were obtained as follows: MXC (working standard) was obtained from a local pharmaceutical company, while PXC (working standard) was obtained from Nantong Jinghua Pharmaceuticals Co LTD (China). Acetonitrile, methanol, phosphoric acid 85%, sodium chloride, potassium chloride, disodium phosphate, potassium dihydrogen phosphate were purchased from Merck (Germany), hydrochloric acid 32% was purchased from Micromchim (Romania) and sodium hydroxide from Chemapol (Czech Republic), all of the reagents had appropriate analytical purity. Human Albumin Grifols 200 g/L, solution for infusion, was purchased from Instituto Grifols SA (Spain).
7
Quantitative Analysis of Analgesics
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Menthol, camphor, and ibuprofen were purchased from P. C. Drug Center Co. Ltd., Bangkok, Thailand. Phosphoric acid 85% was purchased from Merck KGaA, Darmstadt, Germany, and acetonitrile was purchased from V.S. Chem House, Bangkok, Thailand. Potassium dihydrogen phosphate and sodium hydroxide were purchased from QReC, New Zealand, and used as received.
8
Antioxidant Capacity Evaluation Protocol
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Folin–Ciocalteau solution, sodium carbonate, fluorescein, 2,2′-Azobis(2-amidinopropane) dihydrochloride (AAPH), (±)-6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (trolox), gallic acid, methanol (HPLC grade), acetonitrile (LC-MS grade), phosphoric acid 85%, Luperox® TBH70X tert-butyl hydroperoxide solution (TBH), and 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) were purchased from Sigma-Aldrich (Milan, Italy). CellTiter 96® Aqueous One Solution Assay (MTS) was purchased from Promega (Milan, Italy). The water used in all experiments was Millipore Milli-Q.
9
Carotenoid Characterization in Vegetable Oil
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All American Chemical Society (ACS) grade anthrone, copper sulfate pentahydrate, phosphoric acid (85%), sodium hydroxide, sulfuric acid and vanillin, butylated hydroxytoluene, bovine serum albumin, starch, and LC‐MS grade acetone, ethanol, and isopropanol were purchased from Sigma‐Aldrich. LC‐MS grade acetonitrile, methanol, and formic acid were purchased from Fisher Scientific, Loughborough UK. Canola oil was obtained from a local supermarket. Deionized water (DW) was provided by a Milli‐Q system (Millipore), while authentic β‐carotene, β‐cryptoxanthin, echinenone, zeaxanthin, and mixed phytoplankton standards were obtained from DHI lab products, Denmark.
10
Kona Coffee Characterization by HPLC
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Coffee from the Kona region of Hawai’i was sourced from Kona Joe Coffee, LLC (Kealakekua, HI). This single origin Arabica (Kona Typica) coffee was obtained roasted and ground from Kona Joe Coffee following their standard preparation processes. Four coffee types were used in this study. Two roasting temperatures, medium and dark, prepared at two grind sizes, medium and coarse were selected for this work. Both roasting and grinding were done by Kona Joe Coffee. Medium roast beans were roasted at 215 °C to 217 °C, Dark roast beans were roasted at 223 °C to 225 °C. The beans were ground using a Mahlkönig DK-15 industrial grinder.
Standard stock solutions of 400 mg/L caffeine and 3-CGA were made daily and diluted to establish calibration curves for coffee analysis. Both purchased from Sigma Aldrich (Milwaukee, WI). HPLC grade methanol was obtained from Fisher Scientific (Nazareth, PA). Phosphoric acid (85% wt.) was obtained from Sigma Aldrich (Milwaukee, WI) and diluted to 2.0 mM concentration using DI water. Filtered municipal tap water used in this study. Analysis of this water, completed by Penn State University’s Agricultural Analytical Services Laboratory found the water to have a total hardness of 174 mg/L and a pH of 7.5.
Standard stock solutions of 400 mg/L caffeine and 3-CGA were made daily and diluted to establish calibration curves for coffee analysis. Both purchased from Sigma Aldrich (Milwaukee, WI). HPLC grade methanol was obtained from Fisher Scientific (Nazareth, PA). Phosphoric acid (85% wt.) was obtained from Sigma Aldrich (Milwaukee, WI) and diluted to 2.0 mM concentration using DI water. Filtered municipal tap water used in this study. Analysis of this water, completed by Penn State University’s Agricultural Analytical Services Laboratory found the water to have a total hardness of 174 mg/L and a pH of 7.5.
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