BioTek powerWaveTM XS (BioTek Instruments, (Winooski, VT, USA), FP-6200 (JASCO, Easton, MD, USA) and Infinite® 200 PRO multimode (TECAN, Deutschland GmbH, Crailsheim, Germany) microplates readers, convection oven (Romag S.A, Barcelona, Spain), UN 500 universal oven (Memmert, Schwabach, Germany) and Telstar Lyobeta-15 lyophilizer (Telstar, Madrid, Spain) were used for analyses. Forcell culture assays, a Neubauer cell counting chamber (0.100 mm depth, 0.0025 mm2) (Paul Marienfeld GmbH & Co., KG, Lauda-Königshofe, Germany), an incubator (BINDER GmbH, Tuttlingen, Germany), a Thermo Scientific™ MSC-Advantage™ class II biological safety cabinet (Thermo Fisher Scientific, Waltham, MA, USA), an autoclave (3870EA, Tuttnauer, Hauppauge, NY, USA), a TR400-SW TRINO microscope (VWR, Vienna, Austria), CELLSTAR® multiwell culture plates and standard cell culture flasks (Greiner Bio-One GmbH, Kremsmuenster, Austria) were used.
Standard cell culture flasks
Manufactured by Greiner
Sourced in Austria
Standard cell culture flasks are laboratory equipment designed for the in vitro cultivation and propagation of cells. These flasks provide a controlled environment with a flat, transparent surface for the growth and maintenance of cells, tissues, or microorganisms.
Automatically generated - may contain errors
Lab products found in correlation
Powerwavetmxs, by Agilent Technologies (1 mentions)
Un 500 universal oven, by Memmert (1 mentions)
Lyobeta 15 lyophilizer, by Telstar (1 mentions)
Cellstar multiwell culture plates, by Greiner (1 mentions)
Fp 6200, by Jasco (1 mentions)
Imdm medium, by Thermo Fisher Scientific (1 mentions)
Rpmi medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
2 protocols using standard cell culture flasks
1
Multimode Microplate Reader Assays
2
Cell Culture Conditions for Various Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
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CCRF-CEM, Jurkat and NCI-H1299 cells were cultured in RPMI medium (Life Technologies, Darmstadt, Germany) supplemented with 10% foetal bovine serum (FBS, Life Technologies). Cell lines Suit-2 and Capan-1 were cultured in IMDM medium (Life Technologies), 10% FBS. HEK-293T was cultured in IMDM medium, 10% FBS. All cells lines were kept at 37°C, 95% humidity and 5% CO2. Suspension cells were kept at 106 cells/ml and cultured in suspension flasks (Greiner Bio One, Frickenhausen, Germany). Adherent cells were kept at 70–80% confluency and cultured in standard cell culture flasks (Greiner Bio One) while expanding the cell lines.
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