Log In Sign up
The largest database of trusted experimental protocols

Mkeima red mito 7

Manufactured by Addgene
Visit Supplier
Sourced in United States, Germany, China

MKeima-Red-Mito-7 is a mitochondrial targeting fluorescent protein that emits red fluorescence. It is designed for the visualization and study of mitochondrial dynamics in live cells.

Automatically generated - may contain errors

Lab products found in correlation

Pmrfp lc3, by Addgene (2 mentions) Lipofectamine ltx transfection reagent, by Thermo Fisher Scientific (2 mentions) Mito gcamp5g, by Addgene (2 mentions) Percivalhr, by Addgene (2 mentions) Gfp rab7, by Addgene (2 mentions) Egfp lc3, by Addgene (2 mentions) Pcdna3.1 vector, by Thermo Fisher Scientific (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Pbabe puro mcherry egfp lc3b, by Addgene (1 mentions) Pn3 3xflag control, by Addgene (1 mentions) Pbabe egfp, by Addgene (1 mentions) Ecfp ssh1δc, by Addgene (1 mentions) Poptn egfp, by Addgene (1 mentions) Ha ubiquitin, by Addgene (1 mentions) Lipofectamine ltx, by Thermo Fisher Scientific (1 mentions) Juli stage, by NanoEnTek (1 mentions) Lipofectamine 3000, by Beyotime (1 mentions) Confocal microscope, by Olympus (1 mentions) Hoechst 33342, by Beyotime (1 mentions)

Spelling variants of this product

MKeima-Red-Mito-7 plasmid (2 citations)

7 protocols using mkeima red mito 7

1

Autophagy and Actin Dynamics Regulation

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
pN3-3xFlag-Control (Addgene, 107717, Guntram Suske Lab [75 (link)]); pmRFP-LC3 (Addgene, 21075, Tamotsu Yoshimori Lab [43 (link)]); pMXs-puro GFP-Sqstm1/p62 (Addgene, 38277, Noboru Mizushima Lab [49 (link)]); pBABE-puro mCherry-EGFP-LC3B (Addgene, 22418, Jayanta Debnath Lab [76 (link)]); pBABE-EGFP (Addgene, 36999, Debu Chakravarti Lab [77 (link)]); ECFP-SSH1ΔC (N461) (Dr. Mizuno lab [51 (link)]); EGFP-SSH1 (Dr. Storz lab [69 (link)]); mKeima-Red-Mito-7 (Addgene, 56018); pOPTN-EGFP (Addgene, 27052Beatrice Yue Lab [78 (link)]); pMXs-puro GFP-Sqstm1/p62ΔC (Addgene, 38282, Noboru Mizushima Lab [49 (link)]); pMXs-puro GFP-Sqstm1/p62 D337, 338, 339A (GFP-SQSTM1-LIR) (Addgene, 38280, Noboru Mizushima Lab [49 (link)]); HA-Ubiquitin (Addgene, 18712, Edward Yeh Lab [79 (link)]); pDR125 (Addgene, 37150, Dale Ramsden Lab); pN3-3xFlag-SSH1, mCherry-EGFP-Sqstm1/p62, mCherry-EGFP-Sqstm1S403A, mCherry-EGFP-Sqstm1S403E, pN3-Flag-SSH1ΔC, pN3-Flag-SSH1ΔN and pN3-Flag-SSH1ΔNC393S were generated in this work (see methods below).
Fang C., Woo J.A., Liu T., Zhao X., Cazzaro S., Yan Y., Matlack J., Kee T., LePochat P, & Kang D.E. (2020). SSH1 impedes SQSTM1/p62 flux and MAPT/Tau clearance independent of CFL (cofilin) activation. Autophagy, 17(9), 2144-2165.
+ Open protocol
+ Expand
2

Fluorescent Markers for Neuronal Organelles

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
GFP-SNPH and GFP SNPH1–469 plasmids were gifts from Sheng laboratory at NIH. For non-GFP SNPH vectors, we cloned SNPH and SNPH1–469 into pcDNA3.1 vectors (Invitrogen) at HindIII and Xho1 restriction sites. We used Addgene plasmids Mito-GCaMP5G (Addgene plasmid #105009; Kwon et al., 2016 (link)), PercivalHR (Addgene plasmid #49082; Tantama et al., 2013 (link)), mKeima-Red-Mito-7 (Addgene plasmid #56018, a gift from Michael Davidson), EGFP-LC3 (Addgene plasmid #11546; Jackson et al., 2005 (link)) and GFP-RAB7 (Addgene plasmid #12605; Choudhury et al., 2002 (link)). pF(UG) hSyn Lamp1-msGFP (#JV012) plasmid was a gift from Jason Vevea/Edwin R. Chapman at UW, Madison. These plasmids were transfected in primary hippocampal neurons on DIV4 using lipofectamine LTX transfection reagent (Thermo Fisher scientific) for 5 hr as described in manufacturer’s protocol.
Joshi D.C., Zhang C.L., Babujee L., Vevea J.D., August B.K., Sheng Z.H., Chapman E.R., Gomez T.M, & Chiu S.Y. (2019). Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration. Cell reports, 29(3), 685-696.e5.
+ Open protocol
+ Expand
3

Neuronal Transfection for Autophagy and Mitochondrial Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Neurons were cultured on 18 mm diameter coverslips pre-coated with poly-L-Lysine (Sigma-Aldrich, P-1524), and at 4 DIV, they were transfected with 2 µg of plasmid DNA per coverslip using Lipofectamine 2000 (Thermo Fisher Scientific, 11668-019) or Lipofectamine LTX (Thermo Fisher Scientific, 11668-019). A 1:3 ratio of µg plasmid DNA and µL lipofectamine was used. Neurons were incubated with the transfection mixture for 24 h. Plasmid pmRFP-LC3 (Addgene, #21075, Watertown, MA, USA) kindly provided by Dr. Susana Castro Obregón and mKeima-red-mito-7 (Addgene, #56018) were transfected.
Gómora-García J.C., Montiel T., Hüttenrauch M., Salcido-Gómez A., García-Velázquez L., Ramiro-Cortés Y., Gomora J.C., Castro-Obregón S, & Massieu L. (2023). Effect of the Ketone Body, D-β-Hydroxybutyrate, on Sirtuin2-Mediated Regulation of Mitochondrial Quality Control and the Autophagy–Lysosomal Pathway. Cells, 12(3), 486.
+ Open protocol
+ Expand
4

Fluorescent Markers for Neuronal Organelles

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
GFP-SNPH and GFP SNPH1–469 plasmids were gifts from Sheng laboratory at NIH. For non-GFP SNPH vectors, we cloned SNPH and SNPH1–469 into pcDNA3.1 vectors (Invitrogen) at HindIII and Xho1 restriction sites. We used Addgene plasmids Mito-GCaMP5G (Addgene plasmid #105009; Kwon et al., 2016 (link)), PercivalHR (Addgene plasmid #49082; Tantama et al., 2013 (link)), mKeima-Red-Mito-7 (Addgene plasmid #56018, a gift from Michael Davidson), EGFP-LC3 (Addgene plasmid #11546; Jackson et al., 2005 (link)) and GFP-RAB7 (Addgene plasmid #12605; Choudhury et al., 2002 (link)). pF(UG) hSyn Lamp1-msGFP (#JV012) plasmid was a gift from Jason Vevea/Edwin R. Chapman at UW, Madison. These plasmids were transfected in primary hippocampal neurons on DIV4 using lipofectamine LTX transfection reagent (Thermo Fisher scientific) for 5 hr as described in manufacturer’s protocol.
Joshi D.C., Zhang C.L., Babujee L., Vevea J.D., August B.K., Sheng Z.H., Chapman E.R., Gomez T.M, & Chiu S.Y. (2019). Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration. Cell reports, 29(3), 685-696.e5.
+ Open protocol
+ Expand
5

Quantifying Mitophagy in Macrophages

Check if the same lab product or an alternative is used in the 5 most similar protocols
To determine quantitative changes in mitophagy, BMDMs were transfected with mKeima-Red-Mito-7 (Addgene, plasmid 56018). The excitation spectrum of mKeima-Red shifts from 440 to 620 nm when mitochondria are delivered from the cytosol to acidic lysosomes, which occurs during mitophagy (98 (link), 99 (link)). Thirty-six hours after transfection, cell were treated with 100 μM PA or 600 ng/mL LPS for 18 hours and cell images were acquired using the JuLI Stage (NanoEnTek) imaging system with 458 nm (green, mitochondria at neutral pH) and 561 nm (red, mitochondria under acidic pH) lasers. Images were analyzed using ImageJ software.
Moon J.S., da Cunha F.F., Huh J.Y., Andreyev A.Y., Lee J., Mahata S.K., Reis F.C., Nasamran C.A, & Lee Y.S. (2021). ANT2 drives proinflammatory macrophage activation in obesity. JCI Insight, 6(20), e147033.
+ Open protocol
+ Expand
6

Mitochondrial Autophagy Induction in HeLa Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells were transfected with the mKeima-Red-Mito-7 (Addgene, 56018) plasmid using Lipofectamine 3000 for 24 h and then treated with SAHA for another 12 h. The cells were imaged using Leika TCS SP5 Confocal (Ex = 550 nm, Em = 620 nm for acidic red fluorescence; Leica Microsystems, Germany).
Sun X., Shu Y., Ye G., Wu C., Xu M., Gao R., Huang D, & Zhang J. (2021). Histone deacetylase inhibitors inhibit cervical cancer growth through Parkin acetylation-mediated mitophagy. Acta Pharmaceutica Sinica. B, 12(2), 838-852.
+ Open protocol
+ Expand
7

Mitophagy Evaluation in HK-2 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
HK-2 cells were transfected with the mKeima-Red-Mito-7 (Addgene, 56018) plasmid using Lipofectamine 3000 for 40 h and then treated with 8 mg/mL albumin for another 8 h. Nuclei were counterstained with Hoechst 33342 (C1027, Beyotime, China). The cells were imaged using an OLYMPUS confocal microscope (Ex = 550 nm, Em = 620 nm for acidic red fluorescence, Tokyo, Japan). For quantification, more than 20 cells in each group were examined for autolysosome formation to estimate the autolysosome dots/cell.
Duan P., Tan J., Miao Y, & Zhang Q. (2022). PINK1/Parkin-Mediated Mitophagy Plays a Protective Role in Albumin Overload-Induced Renal Tubular Cell Injury. Frontiers in bioscience (Landmark edition), 27(6).
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!