Shandon kwik diff
The Shandon Kwik-Diff is a rapid staining system designed for the preparation of blood smears and other cytological samples. It provides a fast and consistent staining process for the identification of various cell types and structures.
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3 protocols using shandon kwik diff
Tracheal Aspirate Sampling for Gene Expression
Quantifying Airway Inflammation via BALF
Fifty thousand cells were then centrifuged at 400 xg for 5 minutes onto a frosted slide using a Cytospin 3 (Shandon) for differential cell analysis. Slides were fixed with 2-propan-2-ol for 1 minute and left to dry overnight. Slides were then submerged in rapid 1 dye for 12 dips and then immediately placed into rapid II for 6 dips (Shandon Kwik Diff; Thermofisher Scientific). Slides were then left to dry before applying a small drop of DPX mounting media to the cytospot and applying a coverslip. Slides were then imaged under a microscope with 20x magnification. A total of 500 cells were counted from random fields, including macrophages, lymphocytes and neutrophils, based on standard morphological criteria.
Liver and Lung Tissue Analysis
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