Log In Sign up
The largest database of trusted experimental protocols

Anti rankl

Manufactured by R&D Systems
Visit Supplier
Sourced in United Kingdom

Anti-RANKL is a recombinant protein that binds to and neutralizes RANKL. RANKL is a key regulator of osteoclast differentiation and activation, playing a critical role in bone remodeling.

Automatically generated - may contain errors

Lab products found in correlation

Anti tnf α, by R&D Systems (3 mentions) Anti il 1β, by R&D Systems (3 mentions) Anti il 6, by R&D Systems (2 mentions) M csf, by R&D Systems (2 mentions) Vectastain abc kit, by Vector Laboratories (1 mentions) Anti il 17, by R&D Systems (1 mentions) Anti hif 1α, by R&D Systems (1 mentions) Anti vegf, by R&D Systems (1 mentions) N acetyl cysteine (nac), by Merck Group (1 mentions) Anti il 2, by R&D Systems (1 mentions) Recombinant human rankl rhrankl, by R&D Systems (1 mentions) Anti human il 17, by R&D Systems (1 mentions) Anti il 10, by R&D Systems (1 mentions) Recombinant human il 17, by R&D Systems (1 mentions) Il 22, by R&D Systems (1 mentions) Il 21, by R&D Systems (1 mentions) Anti p44 42 mapk erk1 2, by Cell Signaling Technology (1 mentions) Immuno blot pvdf membrane, by Bio-Rad (1 mentions) Anti sm α actin, by Agilent Technologies (1 mentions) Anti mouse igg peroxidase, by Merck Group (1 mentions)

Close spelling variants of this product

RANKL (491 citations) Anti-RANKL Ab (2 citations)

5 protocols using anti rankl

1

Immunohistochemical Analysis of Inflammatory Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fixed joint tissues embedded in paraffin were cut into 7-µm-thick sections, dewaxed using xylene, dehydrated through an alcohol series, and stained with hematoxylin and eosin, safranin O, or toluidine blue to detect proteoglycans. Endogenous peroxidase activity was quenched with methanol/3% H2O2. Immunohistochemistry was performed using a Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA). Sections were incubated with specific antibodies (i.e., anti-TNFα, anti-IL-1β, anti-IL-6, anti-IL-17, anti-RANKL, anti-VEGF, and anti-HIF-1α; all from R&D Systems) overnight at 4 °C. The tissue sections were then incubated with a biotinylated secondary antibody, followed by a streptavidin-peroxidase complex for 1 h. The final color product was developed using diaminobenzidine as the chromogen (DAKO, Carpinteria, CA, USA).
Lee S.Y., Moon S.J., Moon Y.M., Seo H.B., Ryu J.G., Lee A.R., Lee C.R., Kim D.S., Her Y.M., Choi J.W., Kwok S.K., Park S.H, & Cho M.L. (2021). A novel cytokine consisting of the p40 and EBI3 subunits suppresses experimental autoimmune arthritis via reciprocal regulation of Th17 and Treg cells. Cellular and Molecular Immunology, 19(1), 79-91.
+ Open protocol
+ Expand
2

Cytokine Regulation of RANKL-Mediated Osteoclastogenesis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant human RANKL (rhRANKL) and M-CSF were purchased from R&D Systems (Minneapolis, MN, USA). NAC was obtained from Sigma Chemical Co. (St. Louis, MO, USA). Anti-human IL-17, anti-interferon γ (anti-IFN-γ), anti-IL-10, anti-IL-2, anti-IL-1β, anti-TNF-α, anti-IL-6, and anti-RANKL antibodies were purchased from R&D Systems.
Kim H.R., Kim K.W., Kim B.M., Lee K.A, & Lee S.H. (2017). N-acetyl-l-cysteine controls osteoclastogenesis through regulating Th17 differentiation and RANKL in rheumatoid arthritis. The Korean Journal of Internal Medicine, 34(1), 210-219.
+ Open protocol
+ Expand
3

Cytokine-Induced Osteoclastogenesis Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Recombinant human IL-17 (20 ng/mL), IL-21 (20 ng/mL), IL-22 (20 ng/mL), and macrophage colony-stimulating factor (M-CSF) (25 ng/mL) were purchased from R&D Systems (Minneapolis, MN, USA). SKI306X (at 0 μg/mL, 1 μg/mL, 5 μg/mL and 10 μg/mL) were generously provided by the Life Science R&D Center of SK Chemicals (Seongnam, Korea). Anti-TNF-α, anti-IL-1β and anti-RANKL antibodies were purchased from R&D Systems.
Kim H.R., Kim K.W., Kim B.M., Won J.Y., Min H.K., Lee K.A., Kim T.Y, & Lee S.H. (2019). Regulation of Th17 Cytokine-Induced Osteoclastogenesis via SKI306X in Rheumatoid Arthritis. Journal of Clinical Medicine, 8(7), 1012.
+ Open protocol
+ Expand
4

Western Blot Analysis of Vascular Cell Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lysates were produced from VSMC cultures and diluted with Laemmli buffer (62.5mM TRIS-HCl pH 6.8, 25% (w/v) Glycerol, 2% (w/v) SDS and 0.01% (w/v) Bromophenol blue). Protein concentrations were determined using a Micro BCA Protein Assay (Pierce Thermoscientific). Equal quantities of protein were loaded into each well of a Mini-PROTEAN TGX gel (BIO-RAD) and SDS-PAGE was performed using the mini-PROTEAN tetra system (BIO-RAD). Sample proteins were then transferred to an Immuno-blot PVDF membrane (BIO-RAD) and detected using protein specific antibodies, horseradish peroxidase-conjugated antibodies and Pierce ECL Western Blotting Substrate (Pierce Thermoscientific).
The following antibodies were utilized: anti Phospho-p44/42 MAPK (ERK1/2) 1:2000 (Cell signaling), anti p44/42 MAPK (ERK1/2) 1:1000 (Cell signaling), anti phospho-STAT-3 1:2000 (Millipore), anti beta Actin as loading control 1:1000 (Abcam), anti SMα-actin 1:1000 (Dako), anti RANKL (R&D Systems), anti Rabbit IgG Peroxidase Conjugate (Sigma), anti goat IgG HRP (Santa Cruz Technologies, Santa Cruz, CA), anti mouse IgG peroxidase (Sigma).
Callegari A., Coons M.L., Ricks J.L., Rosenfeld M.E, & Scatena M. (2014). Increased Calcification in Osteoprotegerin Deficient Smooth Muscle Cells: Dependence on Receptor Activator of NF-κB Ligand and Interleukin-6. Journal of vascular research, 51(2), 118-131.
+ Open protocol
+ Expand
5

Osteoclast Differentiation and Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Osteoclasts were generated from SFMCs, as previously described (32 (link)). Cultures were stimulated with 25 ng/ml recombinant human (rh) macrophage colony-stimulating factor (M-CSF) (Peprotech, London, UK) and 50 ng/ml rhRANKL (Abcam, Cambridge, UK). At each media change, stimulations were added, including rhPD-1Fc (1 μg/ml) (R&D Systems, Abingdon, UK), rhPD-L1Fc (1 μg/ml) (R&D Systems, Abingdon, UK), or anti-RANKL (denosumab, 5 μg/ml). All cultures were evaluated after 21 days for tartrate-resistant acid phosphatase (TRAP) activity (B-Bridge International, CA, USA). Matrix metalloproteinase 9 (MMP-9) was measured by ELISA at days 4, 10, and 19 (R&D Systems).
Greisen S.R., Kragstrup T.W., Thomsen J.S., Hørslev-Pedersen K., Hetland M.L., Stengaard-Pedersen K., Østergaard M., Ørnbjerg L., Junker P., Sharpe A.H., Freeman G.J., Hvid M., Moestrup S.K., Hauge E.M, & Deleuran B. (2022). The Programmed Death-1 Pathway Counter-Regulates Inflammation-Induced Osteoclast Activity in Clinical and Experimental Settings. Frontiers in Immunology, 13, 773946.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!