The 231MFP cells were obtained from Prof. Benjamin Cravatt and were generated from explanted tumor xenografts of female MDA-MB-231 cells as previously described (Jessani et al., 2004 (link)). The 231MFP cells were cultured in L15 medium containing 10% FBS and maintained at 37 °C with 0% CO2. K562 chronic myeloid leukemia cell lines of female origin were purchased from ATCC. The K562 cells were cultured in Iscove’s Modified Dulbecco’s Medium containing 10% FBS and maintained at 37 °C with 5% CO2. HAP1 RNF114 wild-type and knockout cell lines of female origin were purchased from Horizon Discovery. The RNF114 knockout cell line was generated by CRISPR/Cas9 to contain a frameshift mutation in a coding exon of RNF114. HAP1 cells were grown in Iscove’s Modifed Dulbecco’s Medium in the presence of 10% FBS and penicillin/streptomycin.
Hap1 rnf114 wild type and knockout cell lines
Manufactured by Horizon Discovery
HAP1 RNF114 wild-type and knockout cell lines are genetically engineered cell lines that serve as research tools. The wild-type cell line maintains the original RNF114 gene, while the knockout cell line has the RNF114 gene removed or inactivated. These cell lines can be used to study the role and function of the RNF114 gene in cellular processes.
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3 protocols using hap1 rnf114 wild type and knockout cell lines
1
Cell Line Characterization and Maintenance
2
Culturing Various Cell Lines
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The 231MFP cells were obtained from Prof. Benjamin Cravatt and were generated from explanted tumor xenografts of MDA-MB-231 cells as previously described33 . HCC38 and HEK293T cells were obtained from the American Type Culture Collection. HEK293T cells were cultured in DMEM containing 10% (v/v) fetal bovine serum (FBS) and maintained at 37°C with 5% CO2. 231MFP were cultured in L15 medium containing 10% FBS and maintained at 37°C with 0% CO2. HCC38 cells were cultured in RPMI medium containing 10% FBS and maintained at 37°C with 5% CO2. HAP1 RNF114 wild-type and knockout cell lines were purchased from Horizon Discovery. The RNF114 knockout cell line was generated by CRISPR/Cas9 to contain a frameshift mutation in a coding exon of RNF114. HAP1 cells were grown in Iscove’s Modified Dulbecco’s Medium (IMDM) in the presence of 10 % FBS and penicillin/streptomycin.
3
Culturing Various Cell Lines
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