The cell invasion and migration capacity were analyzed by using the QCMTM 24-Well Colorimetric Cell Migration or Invasion Assay Kit (Millipore, USA). 3×104 stable transfected HCC cells in 300 ml serum-free medium were added to the upper chamber, and 10% FBS-containing medium was used as a chemoattractant in the lower chamber. ECMatrixTM was precoated to the upper chamber for invasion assay, and cells on the bottom of the membrane were stained and checked in 24 h for migration or in 48 h for invasion.
Qcmtm 24 well colorimetric cell migration or invasion assay kit
Manufactured by Merck Group
Sourced in United States
The QCMTM 24-Well Colorimetric Cell Migration or Invasion Assay Kit is a laboratory equipment product that provides a quantitative assessment of cell migration or invasion through a membrane. The kit includes a 24-well plate with inserts, reagents, and a colorimetric detection system to measure the activity of migrating or invading cells.
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3 protocols using qcmtm 24 well colorimetric cell migration or invasion assay kit
1
Cell Invasion and Migration Assay
2
Cell Migration and Invasion Assay
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Cell migration or invasion assay was carried out by using the QCMTM 24-Well Colorimetric Cell Migration or Invasion Assay Kit (Millipore, USA). 3×104 stable transfected SGC-7901 cells in 300μl serum free medium were added to the upper chamber, and 10% FBS-containing medium was used as chemoattractant in the lower chamber. ECMatrixTM was pre-coated to the upper chamber for invasion assay. And cells on the bottom of the membrane were stained and checked after 24h for migration or 48h for invasion.
3
Cell Invasion and Migration Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cell invasion and migration capacity of were analysed using the QCMTM 24‐Well Colorimetric Cell Migration or Invasion Assay Kit (Millipore, USA) according to the methods we previously mentioned.21 3 × 104 stable transfected cells in 300 ml serum‐free medium were added to the upper chamber, and 10% FBS‐containing medium was used as chemoattractant in the lower chamber. ECMatrixTM was pre‐coated to the upper chamber for invasion assay, and cells on the bottom of the membrane were stained and checked after 24 hours for migration or 48 hours for invasion.
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