Mouse anti actin monoclonal antibody

Rabbit anti-Vps28 monoclonal antibody was purchased from Abcam (Cambridge, MA, USA). Mouse anti-Flag monoclonal antibody and rabbit anti-HA polyclonal antibody were purchased from Proteintech (Wuhan, China). Mouse anti-HA monoclonal antibody was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Mouse anti-actin monoclonal antibody was purchased from CWBIO (Beijing, China). Anti-FMDV structural protein polyclonal pig antiserum was prepared by our laboratory by immunizing pigs with FMDV (O/BY/CHA/2010; GenBank accession no. JN998085.1) VLP. Anti-FMDV nonstructural protein 3A polyclonal rabbit antiserum was prepared by our laboratory by immunizing rabbits with FMDV (O/BY/CHA/2010; GenBank accession no. JN998085.1) nonstructural protein 3A. The secondary antibodies conjugated with horseradish peroxidase (HRP), fluorescein isothiocyanate (FITC), and tetramethyl rhodamine isocyanate (TRITC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lipofectamine RNAiMAX and Lipofectamine 2000 were purchased from Invitrogen (California, USA). MG132, CQ, Z-VAD-FMK, and CHX were purchased from MedChemExpress (Monmouth Junction, New Jersey, USA). All drugs in this study were dissolved in 0.1% dimethyl sulfoxide (DMSO).

Mouse anti-HSP60, anti-DDK monoclonal antibody, and rabbit anti-HSP60 monoclonal antibody were purchased from Proteintech (Wuhan, China). Mouse anti-HA monoclonal antibody was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Mouse antiactin monoclonal antibody was purchased from CWBIO (Beijing, China). Rabbit anti-GFP monoclonal antibody was purchased from Abcam (Cambridge, MA, USA). Rabbit anti-HSP10 and anti-Flag monoclonal antibodies and the secondary antibodies conjugated with horseradish peroxidase (HRP), fluorescein isothiocyanate (FITC), and tetramethyl rhodamine isocyanate (TRIC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Polyclonal pig antiserum against FMDV was prepared by our laboratory. An anti-FMDV VP2 monoclonal antibody was kindly provided by Li Yu (Harbin Veterinary Research Institute, Harbin, China). Puromycin and mouse antipuromycin monoclonal antibodies were purchased from Merck Millipore (Billerica, MA, USA). Lipofectamine RNAiMAX and Lipofectamine 2000 were purchased from Invitrogen (California, USA). Mizoribine, MG132, CQ, Z-VAD-FMK, CHX, GSK-3008348, and T-1105 were purchased from MedChemExpress (Monmouth Junction, NJ). All drugs in this study were dissolved in 0.1% DMSO.